Effect of topically applied sildenafil citrate on wound healing: experimental study

Abstract

Wound healing is a complex process that necessitates organization of different cell types and several signalling molecules. The aim of this study is to evaluate the effect of different concentrations of sildenafil citrate, which decreases cGMP degradation, on wound healing by secondary intention.This study was performed using 25 Sprague Dawley rats weighing 200-250 grams. 4 dorsal defects were created. Four different treatment modalities which were 1% and 5% sildenafil citrate gel prepared with carbopol, pure carbopol gel without any drug in it and 0,9% NaCl solution; were applied to each lesion of the same rat. Randomly selected five rats (25 rats in total) were sacrificed on 3rd, 5th, 7th, 10th, and 14th days; and the effect of each modality was evaluated by means of defect area measurement, histopathological examination and measurement of tissue hydroxyproline levels.Sildenafil citrate gel application decreased the defect areas in a dose independent manner starting from 3rd day and dose dependent manner after 7th day. By means of vascularization, sildenafil citrate increased vascularity starting from 3rd day. The strength of acute inflammation was superior in sildenafil groups starting from 5th day; and the amount and maturation of granulation in the wound bed, as well as the strength of chronic inflammation were superior in defects treated with sildenafil citrate as early as 7th day.

The role of nitric oxide during embryonic wound healing

“…Moreover, it was observed for in vitro wound healing assays that NO donors can speed up cell migration and collagen deposition [101, 102]. Application of sildenafil cream (inhibitor of cGMP specific phosphodiesterase type 5 - PDE5) on human epidermal injuries has been found to result in faster wound healing [103]. We were not able to reproduce a significant faster healing in embryos through the use of the NO donor, S-Nitroso-N-acetylpenicillamine (SNAP).…”

Effect of sildenafil citrate on the liver structure and function in obstructive jaundice: An experimental study

“…Kim et al (20) showed that necrosis developing in ischemia-reperfusion damage of NO can be prevented by c-GMP and the guanylate cyclasedependent kinase pathway. Gursoy et al (21) researched the impact of sildenafil on wound recovery and showed that sildenafil makes a positive contribution by increasing the neovascularization and abdominal wall tensile force. Cakir et al (22) stated that sildenafil had a histopathologically positive impact on colon anastomosis recovery.…”

Medida Computadorizada Da Evolução Da Área Cicatricial Tratada Com Ultrassom Terapêutico E Citrato De Sildenafila

“…O fármaco citrato de sildenafila (CS) pode representar uma terapia de resgate em pacientes que não respondem a terapia epitelial convencional. Alternativamente, ele também pode ser a primeira escolha em pacientes nos quais outros medicamentos são contraindicados ou de difícil acesso, concordando com Gürsoy et al (2014) e Farsaei et al (2015 que referem ser o CS um novo agente para tratamento de feridas abertas devido sua facilidade de administração e efeitos colaterais aceitáveis.…”

“…Esse processo inclui o óxido nítrico (NO) que contribui para a vasodilatação e neoangiogênese, além de auxiliar na regulação, proliferação celular, síntese de colágeno e epitelização (FARSAEI et al, 2015). Como o CS aumenta a liberação de NO, está sendo usado para o reparo de lesões (GÜRSOY et al, 2014).…”