Effect of Topical Administration of Monosodium Olpadronate on Experimental Periodontitis in Rats

Goya, Juan A; Paez, Hugo; Mandalunis, Patricia

doi:10.1902/jop.2006.77.1.1

Cited by 25 publications

(32 citation statements)

References 27 publications

(33 reference statements)

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“…### P \ 0.001 vs. experimental periodontitis model widely used to study periodontal disease is by fixing ligatures around the teeth. This animal model has characteristics for the disease process that are similar to those encountered in humans [38][39][40]. In severe degrees of periodontitis, the lesions affect the inter-radicular and inter-dental spaces with extensive alveolar bone resorption and denudations of the molar roots [41].…”

Section: Discussionmentioning

confidence: 99%

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Histamine modulates salivary secretion and diminishes the progression of periodontal disease in rat experimental periodontitis

Prestifilippo

Carabajal

Croci³

et al. 2012

Inflamm. Res.

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Section: Discussionmentioning

confidence: 99%

“…The cotton thread ligature was pushed into the gingival sulcus and was left in place for two weeks until death [32].…”

Section: Methodsmentioning

confidence: 99%

Histamine modulates salivary secretion and diminishes the progression of periodontal disease in rat experimental periodontitis

Prestifilippo

Carabajal

Croci³

et al. 2012

Inflamm. Res.

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“…Each mandible was then processed for paraffin embedding, and 4-m-thick sections were obtained for each. The first section was stained with hematoxylin and eosin (H&E) for histological observations, while the next serial section was stained with tartrate-resistant acid phosphatase (TRAP) to reveal osteoclasts, which are considered to be TRAP-positive multinucleated cells (35)(36)(37). The number of osteoclasts on the interradicular septum (bone surface) (Oc.N/BS) was determined, and the osteoclast surface on the interradicular septum (bone surface) (Oc.S/BS) was evaluated, by scoring the TRAP-positive multinucleated cells attached to the bone surface as defined by Parfitt et al (38,39).…”

Section: Methodsmentioning

confidence: 99%

Inflammatory Bone Loss in Experimental Periodontitis Induced by Aggregatibacter actinomycetemcomitans in Interleukin-1 Receptor Antagonist Knockout Mice

et al. 2014

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cThe interleukin-1 receptor antagonist (IL-1Ra) binds to IL-1 receptors and inhibits IL-1 activity. However, it is not clear whether IL-1Ra plays a protective role in periodontal disease. This study was undertaken to compare experimental periodontitis induced by Aggregatibacter actinomycetemcomitans in IL-1Ra knockout (KO) mice and wild-type (WT) mice. Computed tomography (CT) analysis and hematoxylin-and-eosin (H&E) and tartrate-resistant acid phosphatase (TRAP) staining were performed. In addition, osteoblasts were isolated; the mRNA expression of relevant genes was assessed by real-time quantitative PCR (qPCR); and calcification was detected by Alizarin Red staining. Infected IL-1Ra KO mice exhibited elevated (P, <0.05) levels of antibody against A. actinomycetemcomitans, bone loss in furcation areas, and alveolar fenestrations. Moreover, protein for tumor necrosis factor alpha (TNF-␣) and IL-6, mRNA for macrophage colony-stimulating factor (M-CSF), and receptor activator of NF-B ligand (RANKL) in IL-1Ra KO mouse osteoblasts stimulated with A. actinomycetemcomitans were increased (P, <0.05) compared to in WT mice. Alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN)/bone gla protein (BGP), and runt-related gene 2 (Runx2) mRNA levels were decreased (P, <0.05). IL-1␣ mRNA expression was increased, and calcification was not observed, in IL-1 Ra KO mouse osteoblasts. In brief, IL-1Ra deficiency promoted the expression of inflammatory cytokines beyond IL-1 and altered the expression of genes involved in bone resorption in A. actinomycetemcomitans-infected osteoblasts. Alterations consistent with rapid bone loss in infected IL-Ra KO mice were also observed for genes expressed in bone formation and calcification. In short, these data suggest that IL-1Ra may serve as a potential therapeutic drug for periodontal disease.

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“…Despite the necessity of preventing alveolar bone resorption in such patients, no practical means of inhibition is available, so experimental models of periodontitis play a pivotal role in the development of inhibitors of alveolar bone resorption in periodontitis. Several studies have reported periodontitis in experimental animals and have focused on induction of periodontitis by persistent bacterial challenge via repeated administration of bacteria, bacterial components, or bacterial products (oral gavage model) [1][2][3][4][5][6] or creation of a subgingival environment prone to food impaction by using modifying devices, such as cotton ligatures [7][8][9][10][11][12] . However, many of these experimental models have disadvantages when establishing induced periodontitis.…”

Section: Introductionmentioning

confidence: 99%

A New Experimental Mouse Model of Periodontitis Using an Orthodontic Ligature Wire

Mizuno

Miyazawa

Tabuchi

et al. 2014

J. Hard Tissue Biology.

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Periodontitis is characterized by alveolar bone resorption and loss of attachment. Recently, many induced periodontitis animal models have been developed to mimic alveolar bone resorption, but each model has a number of disadvantages. Periodontitis-inducing procedures should be simple and reproducible. Therefore, with the aim of establishing an experimental mouse model of periodontitis, we induced subgingival plaque accumulation in eight-week-old male C57BL/6 wild-type mice by placing an orthodontic ligature wire around the contact point between the left maxillary first and second molars. Maxillae were collected at four and eight weeks after ligature placement, and alveolar bone between the left molars (test maxillae) was compared with that between the right molars (control maxillae). Micro-computed tomography and pathological analysis were then performed to examine the degree of alveolar bone resorption. Alveolar bone resorption was significant in the test maxillae compared with the controls. In addition, the ligature wire was not found to affect the periodontal tissue. The induction of alveolar bone resorption was likely due to the direct mechanical impact (mechanical irritation of gingiva) of the wire ligature. These findings suggest that placement of the ligature wire around the contact point between teeth successfully induced alveolar bone resorption. Furthermore, use of the ligature wire removed the need to induce alveolar bone resorption by using an impacted wire ligature to create mechanical stress. Therefore, the present report provides a new animal model of periodontitis.

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Effect of Topical Administration of Monosodium Olpadronate on Experimental Periodontitis in Rats

Abstract: The dose used herein was found to inhibit bone loss and to cause marked morphologic changes in osteoclasts. The drug effectively prevented bone loss caused by periodontitis.

Cited by 25 publications

References 27 publications

Histamine modulates salivary secretion and diminishes the progression of periodontal disease in rat experimental periodontitis

Histamine modulates salivary secretion and diminishes the progression of periodontal disease in rat experimental periodontitis

Inflammatory Bone Loss in Experimental Periodontitis Induced by Aggregatibacter actinomycetemcomitans in Interleukin-1 Receptor Antagonist Knockout Mice

A New Experimental Mouse Model of Periodontitis Using an Orthodontic Ligature Wire

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