Effect of Titanium Surfaces on the Osteogenic Differentiation of Human Adipose-Derived Stem Cells

Zanicotti, Diogo Godoy; Duncan, Warwick; Seymour, G. J.; Coates, Dawn

doi:10.11607/jomi.5810

Cited by 10 publications

(9 citation statements)

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“…Following the initial adhesion, which reached a peak for the HA-blasted and bland acid etching implants, a non-significant proliferative phase occurred for all but one implant, namely, the laser treated implants on day three and the double acid etching implants on day seven. This stands in contrast to previous studies on titanium discs [ 7 , 18 , 20 ], and it might be due to the low cell density or the whole-body surface via the presence of solubilized metallic ions with negative effects, which cause inflammation and cytotoxicity as their concentration rises [ 49 , 50 ].…”

Section: Discussioncontrasting

confidence: 64%

“…Implants are mainly classified into four types based on their surface roughness, as determined by the arithmetical mean of the roughness area (Sa), defined as rough (Sa > 2.0 µm), moderately rough (Sa between 1.0–2.0 µm), minimally rough (Sa between 0.5–1.0 µm), or smooth (Sa < 0.5 µm) [ 15 , 16 ]. Many studies employing stem cells in vivo and in vitro in order to understand the mechanism of osseointegration [ 17 , 18 , 19 ] have found that implant titanium discs [ 20 , 21 ] with moderately rough surfaces enable better cell and bone connections than smoother or rougher surfaces [ 15 , 16 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. Surface roughness is also linked to favorable effects on load transmission via the distribution of well-tolerated micro-strains, 0.25–0.50 µε [ 6 , 30 , 31 ], which also favor osteoblastic and progenitor cell differentiation [ 6 , 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

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Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells

Ferro

Azzolin

Spelat

et al. 2022

JFB

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Background: Although the influence of titanium implants’ micro-surface properties on titanium discs has been extensively investigated, the research has not taken into consideration their whole-body effect, which may be considered possible using a combinatorial approach. Methods: Five titanium dental implants with a similar moderate roughness and different surface textures were thoroughly characterized. The cell adhesion and proliferation were assessed after adipose-tissue-derived stem cells (ADSCs) were seeded on whole-body implants. The implants’ inductive properties were assessed by evaluating the osteoblastic gene expression. Results: The surface micro-topography was analyzed, showing that hydroxyapatite (HA)-blasted and bland acid etching implants had the highest roughness and a lower number of surface particles. Cell adhesion was observed after 24 h on all the implants, with the highest score registered for the HA-blasted and bland acid etching implants. Cell proliferation was observed only on the laser-treated and double-acid-etched surfaces. The ADSCs expressed collagen type I, osteonectin, and alkaline phosphatase on all the implant surfaces, with high levels on the HA-treated surfaces, which also triggered osteocalcin expression on day seven. Conclusions: The findings of this study show that the morphology and treatment of whole titanium dental implants, primarily HA-treated and bland acid etching implants, impact the adherence and activity of ADSCs in osteogenic differentiation in the absence of specific osteo-inductive signals.

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Section: Discussioncontrasting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells

Ferro

Azzolin

Spelat

et al. 2022

JFB

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“…The final desired outcome of all bone‐implanted biomaterial is the de novo bone formation connecting the implant to the host bone, termed osseointegration, and the majority of the efforts to develop and test new dental implant modifications rely on tests using osteoblasts or MSCs to predict the implant outcome (Bressel et al, 2017; Fu et al., 2009; Jiang et al., 2015; Lotz et al., 2017; Zanicotti et al., 2018). While these types of tests are important to understand the behavior of bone‐forming cells in response to biomaterial modifications, they ignore the contribution of the other cellular and biological processes that occur in the peri‐implant environment in the 14–21 days before bone formation begins that also affect osseointegration and ultimately dictate the fate of the implanted biomaterial (Biguetti et al., 2018; Vlahović et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

Surface characteristics on commercial dental implants differentially activate macrophages in vitro and in vivo

Abaricia

Shah

Ruzga

et al. 2021

Clinical Oral Implants Res

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Objectives Biomaterial implantation provokes an inflammatory response that controls integrative fate. M2 macrophages regulate the response to implants by resolving the inflammatory phase and recruiting progenitor cells to aid healing. We have previously shown that modified titanium (Ti) disks directly induce M2 macrophage polarization. The aim of this study was to examine macrophage response to commercially available Ti or Ti alloy implants with comparable roughness and varying hydrophilicity. Material and Methods Eleven commercially available Ti (A‐F) or Ti alloy (G‐K) dental implants were examined in this study. Surface topography, chemistry, and hydrophilicity were characterized for each implant. To compare the immune response in vitro, human monocyte‐derived macrophages were seeded on implants and secreted pro‐ and anti‐inflammatory proteins measured. To evaluate the inflammatory response in vivo, mice were subcutaneously instrumented with clinical implants, and implant adherent macrophage populations were characterized by flow cytometry. Results Macrophages on hydrophobic Implant C produced the highest level of pro‐inflammatory proteins in vitro. In contrast, hydrophilic Implant E produced the second‐highest pro‐inflammatory response. Implants F and K, both hydrophilics, produced the highest anti‐inflammatory protein secretions. Likewise, pro‐inflammatory CD80hi macrophages predominated in vivo on implants C and E, and M2 CD206 + macrophages predominated on implants F and K. Conclusions These findings show that hydrophilicity alone is insufficient to predict the anti‐inflammatory effect on macrophage polarization and that other properties—surface composition or topography—determine immune modulation. This in vivo model may be a useful screening method to compare the immunomodulatory response to clinical implants of disparate geometry or size.

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“…One experiment has found that argon plasma modification on the surface of nanocomposite polyurethane scaffolds could have the same effect as before (Griffin et al, 2019). Other studies confirmed that Ti and TiO 2 surfaces have a positive effect on the osteogenesis of ASCs as well (Malec et al, 2016;Zanicotti et al, 2018;Cowden et al, 2019). It may help increase the osteogenic efficiency of ASCs and translate into clinical practice.…”

Section: Physical Stimulationmentioning

confidence: 98%

Current applications of adipose-derived mesenchymal stem cells in bone repair and regeneration: A review of cell experiments, animal models, and clinical trials

Zhang

Yang

Cao

et al. 2022

Front. Bioeng. Biotechnol.

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In the field of orthopaedics, bone defects caused by severe trauma, infection, tumor resection, and skeletal abnormalities are very common. However, due to the lengthy and painful process of related surgery, people intend to shorten the recovery period and reduce the risk of rejection; as a result, more attention is being paid to bone regeneration with mesenchymal stromal cells, one of which is the adipose-derived mesenchymal stem cells (ASCs) from adipose tissue. After continuous subculture and cryopreservation, ASCs still have the potential for multidirectional differentiation. They can be implanted in the human body to promote bone repair after induction in vitro, solve the problems of scarce sources and large damage, and are expected to be used in the treatment of bone defects and non-union fractures. However, the diversity of its differentiation lineage and the lack of bone formation potential limit its current applications in bone disease. Here, we concluded the current applications of ASCs in bone repair, especially with the combination and use of physical and biological methods. ASCs alone have been proved to contribute to the repair of bone damage in vivo and in vitro. Attaching to bone scaffolds or adding bioactive molecules can enhance the formation of the bone matrix. Moreover, we further evaluated the efficiency of ASC-committed differentiation in the bone in conditions of cell experiments, animal models, and clinical trials. The results show that ASCs in combination with synthetic bone grafts and biomaterials may affect the regeneration, augmentation, and vascularization of bone defects on bone healing. The specific conclusion of different materials applied with ASCs may vary. It has been confirmed to benefit osteogenesis by regulating osteogenic signaling pathways and gene transduction. Exosomes secreted by ASCs also play an important role in osteogenesis. This review will illustrate the understanding of scientists and clinicians of the enormous promise of ASCs’ current applications and future development in bone repair and regeneration, and provide an incentive for superior employment of such strategies.

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Effect of Titanium Surfaces on the Osteogenic Differentiation of Human Adipose-Derived Stem Cells

Abstract: This study confirmed that human adipose-derived stem cells could be successfully grown in serum-free, xeno-free culture medium suitable for clinical use. Adipose-derived stem cells thus show potential utility for bone regeneration in association with titanium surfaces.

Cited by 10 publications

References 0 publications

Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells

Assessing the Efficacy of Whole-Body Titanium Dental Implant Surface Modifications in Inducing Adhesion, Proliferation, and Osteogenesis in Human Adipose Tissue Stem Cells

Surface characteristics on commercial dental implants differentially activate macrophages in vitro and in vivo

Current applications of adipose-derived mesenchymal stem cells in bone repair and regeneration: A review of cell experiments, animal models, and clinical trials

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