Effect of time and ultrasonic activation on ethylenediaminetetraacetic acid on smear layer removal of the root canal

Abstract

The aim of this study was to assess the effect of time and the ultrasonic activation of ethylenediaminetetraacetic acid (EDTA) in removing the smear layer of at root canal. A total of 60 bovine inferior incisors were transversely sectioned and instrumented; A smear layer was added in the root dentin surface. The specimen were then divided into six groups according to the following final irrigation protocols: Group (G) 1: conventional irrigation with sodium hypochlorite (NaOCl); G2: conventional irrigation with NaOCl + EDTA for 20 s; G3: conventional irrigation with NaOCl + EDTA for 60 s; G4: passive ultrasonic irrigation (PUI) with NaOCl; G5: PUI with NaOCl + PUI with EDTA for 20 s; G6: PUI + NaOCl and PUI with EDTA for 60 s. Samples were analyzed by scanning electron microscope (SEM) at ×500 increase. The images were measured by three blinded evaluators and classified in scores ranging from 1 to 5. The results were analyzed by the Pearson correlation test and the ANOVA nonparametric Kruskal-Wallis and Dunn's tests were used for the comparisons. Irrigated samples only with NaOCl (G1 and G4) had higher scores when compared with other groups. G5 and G6, irrigated with 17% EDTA and ultrasonic irrigation had lower scores. However, there was no statistically significant difference in G2 and G3, where there was no irrigation. It was concluded that irrigation with 17% EDTA for 20 s promoted the effective removal of smear layer, regardless of ultrasonic irrigation.

Effects of plasma jet, dielectric barrier discharge, photodynamic therapy and sodium hypochlorite on infected curved root canals

“…The lack of difference is probably due to the enlargement of the root canals to ISO 70, but also to the length of only 16 mm and the curvature of about 30°. Studies about the antibacterial effect of sodium hypochlorite in combination with PUI vary in their results , probably because of different experimental designs, such as the application time . In this investigation, NaOCl with PUI was highly effective, which could be due to the previously discussed enlargement and the pretreatment of the specimens where smear layers were removed by EDTA prior to incubation.…”

A eficácia da solução de EDTA na remoção de smear layer e sua relação com o tempo de uso: uma revisão integrativa

“…Frente a esses achados e aos diversos novos mé-todos coadjuvantes de agitação da irrigação, recomenda-se a agitação do EDTA, por um minuto, seguido da agitação do NaOCl, para a remoção efetiva de ambos os componentes, orgânicos e inorgânicos, da camada de smear layer após o preparo biomecâ-nico dos canais radiculares, o que permite uma adequada desinfecção do SCR, sem no entanto alterar a estrutura dentinária 63,64 . Uma nova proposta de ativação do EDTA, por meio da utilização de LASER de baixa potência, também foi avaliada 27,58,[65][66][67][68][69] .…”

Effect of photo-polymerized versus chemical cured glass ionomer based desensitizing agents on patency of dentinal tubules

“…to the whole buccal and lingual surfaces of all specimens in order to remove smear layer, then it was washed away with water and the surface was dried with mini sponge. (8) The prepared 40 surfaces were divided into two groups, 20 each according to the type of desensitizing agent received, The mesial half of each surface received the desensitizing material while the other half was left untreated as a control group knowing that EDTA was applied to both halves the treated and untreated of each buccal and lingual surfaces. Each group was subdivided into four subgroups (N=5), the first subgroup faced an abrasion challenge by A custom made machine that was built to simulate brushing technique (9,10) Fig.…”