Effect of Tilia tomentosa methanolic extract on growth performance, digestive enzyme activity, immune response and haematological indices of common carp (Cyprinus carpio)

Abstract

A B S T R A C TThis study was conducted to determine the effect of dietary supplementation with Tilia tomentosa on the growth performance, digestive enzyme activity, haematological indices and nonspecific immune indices of juvenile common carp (Cyprinus carpio). Fish with an average weight of 4.35 ± 0.16 g were fed a diet supplemented with an aqueous methanolic extract of T. tomentosa at a dose of 0% (control), 0.01%, 0.05% or 0.1% for 45 days. The final weight, weight gain and specific growth rate were observed to be significantly higher for the 00.1% and 0.1% groups compared with the control group (P < 0.05). The feed conversion ratio was significantly decreased in the 0.05% and 0.1% groups compared with the control (P < 0.05). The activities of various digestive enzymes (amylase, lipase and trypsin) were also measured and no significant differences were observed compared to the control (P > 0.05). The mean cell volume of the 0.01% group was significantly increased compared to the control (P < 0.05) and increased lysozyme activity was observed in the 0.05% and 0.1% groups. Respiratory burst activity was significantly increased (P < 0.05) on days 15 and 30 for the 0.1% and 0.05% groups, respectively. No differences were observed for myeloperoxidase activity among the four groups. These results suggest that aqueous methanolic extract of T. tomentosa has a growth-promoting and immunostimulatory effect on common carp. Please cite this paper as follows:Almabrok, A.A., Amhamed, I.D., Mohamed, G.A., Bilen, S., Altief, T.A.S. (2018). Effect of Tilia tomentosa methanolic extract on growth performance, digestive enzyme activity, immune response and haematological indices of common carp (Cyprinus carpio). Marine Science and Technology Bulletin, 7(1): 12-20.

Antioxidant and immunostimulant responses in rainbow trout (Oncorhynchus mykiss) fed with cherry stem extract

“…Regarding ORP, many researchers reported various results on the effects of medicinal plants. Almabrok et al (2018) Haematological responses are important determinants in fish health (Ahmed et al, 2020). In this study, RBC was increased CS-treated fish groups.…”

“…Hence, for prevention or treatment of diseases as well as reducing the effects of stress, the substances that are environment-friendly, economical in use, easily available and natural origins are worthy to use. In this respect, medicinal plants are being studied recently and often used (Almabrok et al, 2018;Karga et al, 2020;Mohamed et al, 2018;Sönmez, Bilen, Albayrak, et al, 2015;Sönmez et al, 2018;Zhu, 2020). Many studies demonstrated beneficial effects and indicated potential application of such herbal products (Arslan et al, 2018;Elbesthi et al,2020;Kesbiç et al, 2020;Parrino et al, 2020;Sönmez, Bilen, Alak, et al, 2015;Ümit et al, 2018;Yılmaz, 2017).…”

Effects of Greek juniper (Juniperus excelsa) extract on immune responses and disease resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss)

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“…Almabrok et al. (2018) reported no difference in MPO activity in carp after treatment with linden ( Tilia tomentosa ). Contrarily, Mohamed et al.…”

Effects of black mustard (Brassica nigra) seed oil on growth performance, digestive enzyme activities and immune responses in rainbow trout (Oncorhynchus mykiss)

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