Effect of Thyme, Clove and Cinnamon Essential Oils on <i>Candida Albicans</i> and Moulds Isolated from Different Sources

Abstract

In this study, mycological examination were conducted on different samples from human, animals and poultry in El-Fayoum and Beni-Suef governorates, Egypt in the period from January to June 2013. The antifungal activities of thyme, clove and cinnamon oils against the recovered fungal isolates were tested using agar dilution method. A total of 209 samples were collected (including vaginal swabs from 18 women, 82 cows and 9 buffaloes beside 100 swabs from broiler chickens; 20 crops and 80 proventriculus). Eighty nine fungal isolates were recovered (40 mold and 49 yeast isolates). Eleven (61.1%) fungal isolates were recovered from women; of which 1 isolate was A. flavus and 10 were Candida species while 39 (42.9%) fungal isolates were recovered from animals; of which 36 were molds and 3 were C. albicans. In broiler chickens, 39 (39%) fungal isolates were recovered; of which 36 were yeast; 34 Candida species and 2 Cryptococcus species and 3 were molds; 2 A. niger and 1 A. flavus. PCR assay using oligonucleotide primer that amplifying 172bp fragment in SAP3 gene of C. albicans confirmed morphological, biochemical and biological identification of C. albicans. The antifungal activities of the tested essential oils against the recovered fungi revealed that thyme oil completely inhibited the growth of different fungal isolates at concentrations of 0.25, 0.5 and 1%. Clove and cinnamon oils completely inhibited the growth of different fungal isolates at a concentration of 6%.

Characterization, Virulence Factors and Antifungal Susceptibility of Vulvovaginal Candida Isolated from Women at Qena, Egypt

“…The essential oils contain a variety of volatile molecules including terpenes, terpenoids, aldehydes, ketones, lactones and phenolic compounds which have antifungal consequences (Bakkali et al, 2008 andAkthar et al, 2014). Several studies have been emitted to clarify the effect of essential oils and their main compounds on different Candida species (Benlafya et al, 2014;Radwan et al, 2014 andKaro et al, 2017).…”

Antifungal Activity of some Essential Oils on Cotton Fabrics

“…Antimicrobial activities of essential oils extracted from plants have been reported by: Nzeako et al, 2006, showing antimicrobial activities of clove and thyme extracts; Chee and Lee (2007) showed antifungal activity of clove essential oil and its volatile vapour against Dermatophytic Fungi; Radwan et al (2014) showed in a study regarding antifungal activity of thyme, clove and cinnamon essential oils on Candida albicans and moulds isolated from different sources that thyme oil completely inhibited the growth of different fungal isolates at concentrations of 0.25, 0.5 and 1%; Surdu et al (2014) studied improvement of the resistance to Candida albicans and Trichophyton interdigitale of some woven fabrics based on cotton.…”

Footwear Protection against Fungi Using Thyme Essential Oil

“…Studies prove conclusively that certain essential oils work as powerful antifungals [3][4][5]. Some essential oils have the ability to kill parasites and fungal infections, which is why they are so popular for use in fighting toenail fungus, ringworm and athlete's toenail infection.…”