Effect of the Proteolytic Enzymes of Bacillus licheniformis and the Lysoamidase of Lysobacter sp. XL1 on Proteus vulgaris and Proteus mirabilis Cells

Abstract

Preparations of culture liquid of three Bacullus licheniformis strains (S, 103, and 60.4) and the enzymatic preparation lysoamidase from culture liquid of Lysobacter sp. strain XL1 actively lysed pre-autoclaved cells of the gram-negative bacteria Proteus vulgaris and P. mirabilis. Living Proteus cells treated with these enzymatic preparations were lysed during their subsequent autoclaving. Inoculation of enzyme-treated Proteus cells, taken either separately or in combination with one another and polymyxin B, into a rich medium led to cell repair and restoration of the culture viability.

Green Synthesis and Characterization of Novel Silver Nanoparticles Using Achillea maritima subsp. maritima Aqueous Extract: Antioxidant and Antidiabetic Potential and Effect on Virulence Mechanisms of Bacterial and Fungal Pathogens

“…The reaction mixture contained v / v of the bacterial cell wall solution and AgNPs (50 µg/mL), and was incubated at 37 °C for 60 min. The OD was measured at 600 nm, and the decrease in OD with 0.001/mL/min corresponded to 1AU of lysozyme activity [ 46 ].…”

Modern Status and Perspectives of Bacillus Bacteria as Probiotics

“…Fibrinolytic enzyme, produced by B. vallismortis showed high efficacyin lysis of Streptococcus mutants [27]. Some Bacilluslytic enzymes are active against Gram-negative bacteria [28], others have broad spectrum of activity, including Gram-positive and Gramnegative bacteria [29,30]and yeasts [31].…”