Effect of the presence of a CL in the ovary on oocyte number, cleavage rate and blastocyst production in vitro in cattle

Boediono, Arief; Rajamahendran, R.; Saha, Sukumar; Sumantri, Cece; Suzuki, Takahiro

doi:10.1016/0093-691x(95)92323-2

Cited by 28 publications

(13 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In animals that underwent OPU, the punctured follicles could develop into luteal structures (Gibbons et al., 1994; Boni et al., 1997; Kurykin et al., 1997). The presence of a corpus luteum (CL) producing progesterone might affect the OPU results, with regard to both the number of recovered oocytes and their quality (Boediono et al., 1995; Lansbergen et al., 1995; Stubbings and Walton, 1995). The endocrine events leading to the formation of CL‐like structures after follicular puncture, in particular, are therefore of interest for further studies, as well as the circumstances at luteinization.…”

Section: Introductionmentioning

confidence: 99%

Effects of Repeated Follicular Punctures on Ovarian Morphology and Endocrine Parameters in Dairy Heifers

Petyim

Båge

Forsberg

et al. 2001

Journal of Veterinary Medicine Series A

View full text Add to dashboard Cite

Three experimental and three control heifers were included in the study. After a control period (CP), the experimental heifers were subjected to 4 and 5 weeks of twice-weekly follicular punctures (FPP1 and FPP2) with two oestrous cycles in between. The follicular punctures were performed using the ovum pick-up (OPU) technique. During the CP and FPPs, ovarian activity was monitored by transrectal ultrasonography and analyses of plasma progesterone, prostaglandin F2alpha (PGF2alpha) metabolite, luteinizing hormone (LH), oestradiol and follicle stimulating hormone (FSH) levels. The animals were slaughtered 7 days after cessation of FPP2 and their ovaries examined. The control animals were monitored for their oestrous cyclicity and were then ovariectomized and the ovaries examined. Despite the absence of ovulation, the formation of corpus luteum (CL)-like structures could be ultrasonographically observed during FPPs. These structures either had characteristics comparable to those of the CLs during the CP (group 1) or were smaller and had a shorter life span and (or) luteal active phase with a lower progesterone production (group 2). In group 1, CL-like structures emanated from punctures of large-sized follicles on or close to the days on which the animals showed oestrus and had a rise in oestradiol and LH. In group 2, the structures developed from punctures of smaller-sized follicles and no oestrus nor a detectable 1.14 rise was revealed around punctures. During most FPPs, the basal level of LH was at zero. During all FPPs, a significant rise in FSH level occurred on the day following the day of puncture. To conclude, repeated follicular puncture appeared to alter slightly endocrine profiles and cause minor morphological changes in the ovaries. Both the macro- and microscopic examinations revealed the presence of luteal structures and follicles of various sizes in the ovaries of all heifers studied. The connective tissue in the ovarian tunica albuginea of the experimental heifers was significantly thicker than that in controls (P < 0.001).

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of Repeated Follicular Punctures on Ovarian Morphology and Endocrine Parameters in Dairy Heifers

Petyim

Båge

Forsberg

et al. 2001

Journal of Veterinary Medicine Series A

View full text Add to dashboard Cite

show abstract

“…In animals subjected to OPU, the punctured follicles can develop into luteal structures (Gibbons et al, 1994; Kurykin et al, 1997). The presence of a CL producing progesterone can influence OPU results, in terms of both the number of recovered oocytes and their quality (Boediono et al, 1995; Lansbergen et al, 1995; Stubbings and Walton, 1995). Therefore, the purpose of the present study was to study the effect of repeated follicular puncture on ovarian structure and function in dairy heifers, particularly the luteinization of the punctured follicles.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Repeated Follicular Puncture on Ovarian Function in Dairy Heifers

Petyim¹,

Båge²,

Forsberg

et al. 2000

Journal of Veterinary Medicine Series A

View full text Add to dashboard Cite

Three dairy heifers were examined during three consecutive oestrous cycles (control period, CP). Subsequently, the animals were subjected to 4 and then 5 weeks of twice-weekly ovum pick-up (OPU) (FPP1 and FPP2, respectively) with a recovery period (RP) of two consecutive oestrous cycles between FPP1 and FPP2. After FPP2, the animals were slaughtered and the ovaries were macroscopically examined. Throughout, ovarian activity was monitored by transrectal ultrasonography and concentrations of plasma progesterone. During CP, all the heifers showed normal cyclicity. During FPPs, the heifers occasionally presented oestrous activity. Corpus luteum (CL)-like structures developed from punctured follicles with diameters and life-spans varying from smaller and shorter than those in the CP (P > 0.05) to equal to those in the CP. There was a tendency for a lower number of emerging and punctured follicles in the presence of a CL-like structure. Subsequently to FPP1, all heifers regained normal cyclicity. A thickening of the ovarian tunica albuginea and a slight hardening of the ovaries were found postmortem. In conclusion, dairy heifers can occasionally show cyclic activity and form CL-like structures during twice-weekly OPU. Further, OPU did not seem to cause any major negative effects on ovarian structure and subsequent ovarian function.

show abstract

“…In domestic animals, three methods for collection of oocytes have been described: aspiration of the oocyte from the follicles of living cows (a procedure also called Ovum Pick-up -OPU) (Datta et al, 1993;Boediono et al, 1995), slicing the ovaries (Carolan et al, 1992;Mogas et al, 1992;Pawshe et al, 1994) and puncture of visible surface follicles from slaughtered cows (Wani et al, 1999;Shirazi et al, 2005). The slicing technique yields more oocytes per ovary than follicle aspiration technique (Das et al, 1996).…”

Section: Resultsmentioning

confidence: 99%

In Vitro Production of Embryos at Research and Development Station for Cattle Breeding Dancu, Iasi – First Bovine Embryos Produced In Vitro in North-Eastern Romania

Borş¹,

Creangă²,

Dascalu³

et al. 2017

Cercetari Agronomice in Moldova

View full text Add to dashboard Cite

ABSTRACT. The in vitro production (IVP) of bovine embryos increases the selection intensity in cattle and reduces the generation interval, which is very important in the genetic gain. In Romania, this reproductive biotechnology has shown a timid evolution in the last years, although the need for genetic improvement in the area is present. The aim of this paper is to describe the work that resulted in first bovine embryos obtained through IVP in North-Eastern Romania. Oocytes were collected by slashing ovaries from slaughtered cows, matured in a TCM199-based medium and fertilized in TL-based medium microdrops with sperm processed by swim-up procedure. The presumptive embryos were cultured one day in TCM199 and 8 days in SOF-based medium and evaluated in days 7, 8 and 9 after fertilization. We retrieved an average number of 83 usable oocytes/IVF session, which represents 73.8% from the total harvested oocytes. The average number of cleaved embryos was 50.8 per IVF, reflecting an average cleavage rate of 61.2%. An average of 8.6 blastocysts/IVF session was obtained, representing 10.4% of the selected oocytes or 16.9% of the number of cleaved embryos. Although suboptimal, the results were comparable with other reports on IVP in cattle. The adapted IVP protocol, based on maturation with TCM199, fertilization in microdrops of TL and culture of presumptive embryos one day in TCM199 and afterwards in SOF seems to offer acceptable results and will be used for further attempts to produce bovine embryos.Keywords: IVP; TCM199 medium; TL medium; SOF medium; swim-up. REZUMAT. Producerea in vitro S.I. BORŞ ET AL.110 creștere a intensității de selecție a bovinelor și o reducere a intervalului între generații, elemente importante în realizarea câștigului genetic. În România, această biotehnică de reproducere asistată la taurine a prezentat o evoluție timidă în ultimii ani, deși există o nevoie crescută în ceea ce privește ameliorarea șeptelului de bovine pentru lapte. Scopul acestui studiu este de a descrie rezultatele obținute ca urmare a cercetărilor efectuate pentru producerea primilor embrioni in vitro la specia Bos taurus în zona de Nord-Est a României. Ovocitele au fost recoltate prin realizarea unor incizii multiple în corticala ovarelor provenite de la vaci sacrificate în abator, apoi maturate în mediul TCM 199 și fertilizate în mediul TL, cu material seminal procesat prin metoda swim-up. Embrionii au fost apoi cultivați o zi în mediul TCM 199 și 8 zile în mediul SOF, evaluarea lor făcându-se pe parcurs în zilele 7, 8 și 9 după fertilizare. S-a obținut o medie de 83 ovocite de bună calitate recoltate/sesiune fertilizare in vitro (FIV), ceea ce reprezintă 73,8% din totalul ovocitelor recoltate. Numărul mediu de embrioni divizați a fost de 50,8/sesiune FIV, ceea ce reflectă o rată de clivaj de aproximativ 61,2%. Din totalul acestora s-a obținut un număr mediu de 8,6 blastociști/sesiune FIV, reprezentând 10,4% din totalul ovocitelor selectate sau 16,9% din numărul de embrioni clivați. Deși rezultatele nu sunt optime, pot f...

show abstract

Effect of the presence of a CL in the ovary on oocyte number, cleavage rate and blastocyst production in vitro in cattle

Cited by 28 publications

References 0 publications

Effects of Repeated Follicular Punctures on Ovarian Morphology and Endocrine Parameters in Dairy Heifers

Effects of Repeated Follicular Punctures on Ovarian Morphology and Endocrine Parameters in Dairy Heifers

The Effect of Repeated Follicular Puncture on Ovarian Function in Dairy Heifers

In Vitro Production of Embryos at Research and Development Station for Cattle Breeding Dancu, Iasi – First Bovine Embryos Produced In Vitro in North-Eastern Romania

Contact Info

Product

Resources

About