Effect of the phytotherapeutic agent eviprostat on the bladder in a rat model of bladder overdistension/emptying

Abstract

Eviprostat-mediated suppression of increased bladder oxidative stress and inflammation caused by bladder OE may contribute to the improvement of BBF and bladder function by Eviprostat.

Effect of a single treatment with tadalafil on blood flow in lower urinary tract tissues in rat models of bladder overdistension/emptying and abdominal aorta clamping/release

“…In one model, we subjected rats to continuous bladder distension for 2 h followed by emptying and noted that BBF decreased progressively after emptying. This result is consistent with our previous finding of a decreased BBF the day after the induction of bladder O/E (Kawai et al, 2013). In this model, we found that intraduodenal administration of tadalafil significantly blocked the progressive O/ E-induced reduction in BBF.…”

“…These findings indicate that bladder ischemia is a key mechanism underlying symptoms that are associated with BPH. Although the decreased BBF observed in our acute rat model may not reflect exactly the chronic decrease in BBF in human BPH, bladder O/E carried out under conditions similar to those of our model have been used by us and others to study the effect of drugs on BBF and bladder dysfunction (Kawai et al, 2013;Okutsu et al, 2011). Therefore, it is likely that tadalafil increased the blood supply and reduced the bladder ischemia caused by BOO.…”

“…Surgery for bladder O/E was carried out as described by Kawai et al (2013). Female rats were anesthetized intraperitoneally with pentobarbital (50 mg/kg).…”

“…BBF and PBF were determined with a laser speckle blood flow imager (Omegazone OZ-1; Omegawave, Tokyo, Japan) as described previously (Kawai et al, 2013). With the rat under pentobarbital anesthesia, the ventral prostate and bladder were exposed through an abdominal midline incision to allow measurement.…”

Bladder function in 17β-estradiol-induced nonbacterial prostatitis model in Wister rat

“…The bladder was exposed and emptied by inserting a catheter through the urethra. After removing the catheter, the BBF was determined with a laser speckle blood flow imager (Omegazone OZ-1; Omegawave, Tokyo, Japan) as described by Oka et al [7]. For measurement, the bladder surface was diffusely illuminated with a 780-nm semiconductor laser, scattered light was filtered with a hybrid filter and detected by a charge-coupled-device (CCD) camera, and the images were transferred to a computer for analysis.…”