Effects of the phosphodiesterase 5 inhibitor Tadalafil on bladder function in a rat model of partial bladder outlet obstruction

Kawai, Yuko; Oka, Michiko; Yoshinaga, Ryohei; Fuchikami, Chiaki; Oyama, Tatsuya

doi:10.1002/nau.22732

Cited by 25 publications

(31 citation statements)

References 32 publications

(47 reference statements)

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“…We suspended tadalafil in a 0.5% methylcellulose solution, using an agate mortar and pestle, to create a 2 mg/mL solution; the drug was administered (1 mL/kg body weight) according to a previous tadalafil report . Six weeks after inducing diabetes, rats in the DT group received oral tadalafil (2 mg/kg/day) daily for 7 days.…”

Section: Methodsmentioning

confidence: 99%

Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats

Gotoh

Torimoto

Tatsumi

et al. 2017

Neurourology and Urodynamics

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Section: Methodsmentioning

confidence: 99%

Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats

Gotoh

Torimoto

Tatsumi

et al. 2017

Neurourology and Urodynamics

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“…Given that the expression of PDE5 mRNA is observed in both iliac and vesical arteries, previous reports have shown that chronic treatment with tadalafil increased BBF by inhibiting PDE5. 19,20 Furthermore, chronic treatment with 2 mg/kg tadalafil improved bladder dysfunction in the chronic bladder ischemic rat model. 6 Kawai et al reported that chronic treatment with 10 mg/kg tadalafil ameliorated bladder dysfunction in the bladder outlet obstruction rat model.…”

Section: Discussionmentioning

confidence: 94%

“…The major artery that supplies blood to the rat bladder is the vesical artery, a branch of the internal iliac artery. Given that the expression of PDE5 mRNA is observed in both iliac and vesical arteries, previous reports have shown that chronic treatment with tadalafil increased BBF by inhibiting PDE5 . Furthermore, chronic treatment with 2 mg/kg tadalafil improved bladder dysfunction in the chronic bladder ischemic rat model .…”

Section: Discussionmentioning

confidence: 95%

Effects of silodosin and tadalafil on bladder dysfunction in spontaneously hypertensive rats: Possible role of bladder blood flow

et al. 2020

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Objectives To investigate the effects of an alpha1‐adrenoceptor antagonist, silodosin, or a phosphodiesterase type 5 inhibitor, tadalafil, on bladder overactivity in spontaneously hypertensive rats. Methods Twelve‐week‐old male spontaneously hypertensive rats were perorally administered silodosin (100 µg/kg), tadalafil (2 or 10 mg/kg) or vehicle once daily for 6 weeks. Wistar rats were used as normotensive controls and were treated with the vehicle. At 18‐weeks‐old, the effects of silodosin or tadalafil on blood pressure, bladder blood flow, urodynamic parameters (i.e. micturition frequency, urine output, inter‐contraction interval, maximum voiding pressure, single voided volume and post‐voiding residual urine volume), and bladder tissue levels of malondialdehyde, interleukin‐6 and tumor necrosis factor‐alpha were measured. Results A significant increase in blood pressure, micturition frequency and bladder tissue levels of malondialdehyde, interleukin‐6 and tumor necrosis factor‐alpha was noted in spontaneously hypertensive rats. The single voided volume, bladder capacity and bladder blood flow were significantly lower in the spontaneously hypertensive rats than in the Wistar rats. Treatment with silodosin and the higher dose of tadalafil improved the urodynamic parameters, bladder blood flow and bladder tissue levels of malondialdehyde in the spontaneously hypertensive rats without affecting the blood pressure and bladder tissue levels of interleukin‐6 and tumor necrosis factor‐alpha. Conclusions Treatment with silodosin or tadalafil might improve hypertension‐related bladder overactivity, as shown in spontaneously hypertensive rats through an improvement in the bladder blood flow and bladder tissue levels of oxidative stress.

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“…Tadalafil was suspended in 0.5% methylcellulose solution using an agate mortar and pestle to obtain a 2 mg/mL solution. Rats were administered tadalafil (1 mL/kg body weight) according to a previous report . Briefly, tadalafil (2 mg/kg/d) was administered orally to rats in the DT group for 7 days from Week 6 to Week 7 after the induction of diabetes.…”

Section: Methodsmentioning

confidence: 99%

Tadalafil, a phosphodiesterase type 5 inhibitor, restores urethra and detrusor function in the initial phase of diabetes in rats

Gotoh

Torimoto

Iwasaki

et al. 2019

LUTS

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Objective This study investigated the effects of tadalafil on the urethra and detrusor in the initial phase of diabetes in rats. Methods Thirty‐six female Sprague‐Dawley rats were assigned to a non‐diabetes (ND), diabetes (D), or tadalafil‐treated diabetes (DT) group (n = 12 per group), with the DT group receiving oral tadalafil (2 mg/kg/d) for 7 days before the experiments. Seven weeks after diabetes induction (by a single intraperitoneal injection of streptozotocin), urethral and intravesical pressure were simultaneously recorded in vivo, whereas responses of detrusor strips to potassium chloride (30 mM), electrical field stimulation (EFS) and carbachol were measured in vitro. Results The intravesical pressure at which the urethra started to relax was significantly lower in the DT than D group (mean [± s.d.] 18.9 ± 2.9 vs 29.1 ± 6.6 cm H2O; P < .05). In addition, the reduction in urethral pressure was significantly larger in the DT than D group (−10.9 ± 4.0 vs −4.0 ± 2.9 cm H2O; P < .05). Detrusor stimulation revealed that the mean contractile responses to EFS and carbachol were significantly lower in the ND and DT groups than in the D group (120.7 ± 26.5% and 130.8 ± 15.8% vs 200.1 ± 47.9% of the 30 mM KCl‐induced contraction, respectively, in response to 50 Hz EFS [P < .05]; 211.1 ± 35.4% and 208.4 ± 25.3% vs 425.7 ± 125.0% of the 30 mM KCl‐induced contraction, respectively, in response to 10−3 M carbachol [P < .05]). Conclusions Tadalafil restored urethral relaxation function and detrusor responses to EFS and carbachol during the initial phase of diabetes.

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Effects of the phosphodiesterase 5 inhibitor Tadalafil on bladder function in a rat model of partial bladder outlet obstruction

Abstract: Tadalafil suppressed the reduction in bladder blood flow caused by BOO in rats and improved urinary function. This action of Tadalafil may contribute to its amelioration of bladder function. Neurourol. Urodynam. 35:444-449, 2016. © 2015 Wiley Periodicals, Inc.

Cited by 25 publications

References 32 publications

Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats

Tadalafil, a phosphodiesterase type 5 inhibitor, improves bladder blood supply and restores the initial phase of lower urinary tract dysfunction in diabetic rats

Effects of silodosin and tadalafil on bladder dysfunction in spontaneously hypertensive rats: Possible role of bladder blood flow

Tadalafil, a phosphodiesterase type 5 inhibitor, restores urethra and detrusor function in the initial phase of diabetes in rats

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