Effect of the pH value on the thermal stability of alkaline phosphatase

Atyaksheva, L. F.; Poltorak, O. M.; Chukhrai, E. S.; Fedosov, S. A.

doi:10.1134/s0036024406040273

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Cited by 3 publications

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“…The Fe 3 O 4 MNPs used in the experiment are stable way beyond the duration of the experiment 28 and, similarly, the AP enzyme is stable under the chosen pH and temperature conditions. 29 Fig. 4 shows the elemental composition of the nonmagnetic precipitate obtained from the enzymatic procedure as measured by SEM/EDX.…”

Section: Methods Detection Limitmentioning

confidence: 99%

Enzymatic conversion of magnetic nanoparticles to a non-magnetic precipitate: a new approach to magnetic sensing

Kolhatkar

Jamison

Nekrashevich

et al. 2016

Analyst

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Magnetic sensing utilizes the detection of biomolecule-conjugated magnetic nanoparticles (MNPs). Our new strategy offers a novel approach to magnetic sensing where in situ conversion produces a "loss of signal" in the sensing device. This report demonstrates the enzymatic conversion of Fe3O4 MNPs to a non-magnetic precipitate via reduction by l-ascorbic acid generated by the action of alkaline phosphatase.

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Section: Methods Detection Limitmentioning

confidence: 99%