Formation of calcium phosphate coatings within polycaprolactone scaffolds by simple, alkaline phosphatase based method

Jaroszewicz, Jakub; Idaszek, Joanna; Choińska, Emilia; Szlązak, Karol; Hyc, Anna; Osiecka-Iwan, Anna; Święszkowski, Wojciech; Moskalewski, Stanisław

doi:10.1016/j.msec.2018.11.027

Cited by 21 publications

(19 citation statements)

References 41 publications

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“…Recently, two key routes of scaffold fabrication have been developed. The first involves the production of matrices with previously designed three-dimensional architecture, e.g., by 3D printing [ 16 , 17 , 18 , 19 ], macro-porous hydrogels synthesis [ 20 , 21 , 22 ], or electrospinning [ 23 , 24 ]. The second uses renewable decellularized natural sources of prefabricated scaffolds in their original shape, e.g., autogenic or allogenic ECM [ 25 , 26 , 27 ], skeletons with proper architecture [ 28 , 29 ] or plant-based structure [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

et al. 2021

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Tissue engineering (TE) is a field of regenerative medicine that has been experiencing a special boom in recent years. Among various materials used as components of 3D scaffolds, naturally formed chitinous materials seem to be especially attractive because of their abundance, non-toxic and eco-friendly character. In this study, chitinous skeleton isolated from the marine sponge Aplysina fistularis (phylum: Porifera) was used for the first time as a support for the cultivation of murine fibroblasts (Balb/3T3), human dermal fibroblasts (NHDF), human keratinocyte (HaCaT), and human neuronal (SH-SY5Y) cells. Characterization techniques such as ATR FTIR, TGA, and μCT, clearly indicate that an interconnected macro-porous, thermostable, pure α-chitin scaffold was obtained after alkali–acid treatment of air-dried marine sponge. The biocompatibility of the naturally formed chitin scaffolds was confirmed by cell attachment and proliferation determined by various microscopic methods (e.g., SEM, TEM, digital microscopy) and specific staining. Our observations show that fibroblasts and keratinocytes form clusters on scaffolds that resemble a skin structure, including the occurrence of desmosomes in keratinocyte cells. The results obtained here suggest that the chitinous scaffold from the marine sponge A. fistularis is a promising biomaterial for future research about tissues regeneration.

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Section: Introductionmentioning

confidence: 99%

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

et al. 2021

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“…According to Jaroscewicz et al [ 20 ], it is possible to produce an HA layer with the help of ALP. ALP present in the body catalyzes the hydrolysis of phosphate monoesters.…”

Section: Methodsmentioning

confidence: 99%

The Mineralization of Various 3D-Printed PCL Composites

Egorov

Riedel

Vinke

et al. 2022

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In this project, different calcification methods for collagen and collagen coatings were compared in terms of their applicability for 3D printing and production of collagen-coated scaffolds. For this purpose, scaffolds were printed from polycaprolactone PCL using the EnvisionTec 3D Bioplotter and then coated with collagen. Four different coating methods were then applied: hydroxyapatite (HA) powder directly in the collagen coating, incubation in 10× SBF, coating with alkaline phosphatase (ALP), and coating with poly-L-aspartic acid. The results were compared by ESEM, µCT, TEM, and EDX. HA directly in the collagen solution resulted in a pH change and thus an increase in viscosity, leading to clumping on the scaffolds. As a function of incubation time in 10× SBF as well as in ALP, HA layer thickness increased, while no coating on the collagen layer was apparently observed with poly-L-aspartic acid. Only ultrathin sections and TEM with SuperEDX detected nano crystalline HA in the collagen layer. Exclusively the incubation in poly-L-aspartic acid led to HA crystals within the collagen coating compared to all other methods where the HA layers formed in different forms only at the collagen layer.

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“…This effect is associated with the increased release of the ions from the β-TCP component 145,155 . scaffolds prepared by porogen leaching 160,161 , electrospun nanofibrous mat 162,163 , and 3D printed scaffolds 164,165 . Treatment of a porous composite PLA/HA scaffold with air plasma improved the nucleation of apatite when the scaffolds were incubated in SBF.…”

Section: Calcium Phosphate-based Ceramicsmentioning

confidence: 99%

Surface engineering of biodegradable implants: emerging trends in bioactive ceramic coatings and mechanical treatments

2021

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Formation of calcium phosphate coatings within polycaprolactone scaffolds by simple, alkaline phosphatase based method

Cited by 21 publications

References 41 publications

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

The Mineralization of Various 3D-Printed PCL Composites

Surface engineering of biodegradable implants: emerging trends in bioactive ceramic coatings and mechanical treatments

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