Effect of the oxygen supply on pattern of growth and corrinoid and organic acid production of Propionibacterium shermanii

Quesada-Chanto, A.; Silveira, Maurício Moura da; Schmid-Meyer, A. C.; Schroeder, A. G.; Costa, Jéssica Pereira; López, José Antonio Fernández; Carvalho-Jonas, Maria F.; Artolozaga, M. J.; Jonas, Rainer

doi:10.1007/s002530051239

Cited by 11 publications

(8 citation statements)

References 16 publications

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“…The maximal concentration of LA (4.82 g/L) was obtained at À400 mV, and the maximal concentration of AA (3.48 g/L) was obtained at À100 mV. Though the higher biomass could be obtained under aerobic conditions, the production of PA decreased significantly (Quesada-Chanto et al, 1998).…”

Section: Pa Production With Various Levels Of Orp Controlmentioning

confidence: 99%

Improved propionic acid production with metabolically engineered Propionibacterium jensenii by an oxidoreduction potential-shift control strategy

Zhuge

Shin

et al. 2015

Bioresource Technology

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Section: Pa Production With Various Levels Of Orp Controlmentioning

confidence: 99%

Improved propionic acid production with metabolically engineered Propionibacterium jensenii by an oxidoreduction potential-shift control strategy

Zhuge

Shin

et al. 2015

Bioresource Technology

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“…Consistent with its facultative anaerobic nature, P. freudenreichii has a structurally complete TCA cycle 45,48−50, which may justify the observed oxidative phenotypes with free oxygen and the ability of the strain to grow under aerobic conditions [51][52][53][54] . Genome annotation data suggest that strain DSM20271 contains all the enzymes of the electron transport chain essential for both anaerobic and aerobic metabolism, including NADH dehydrogenase/complex I (NDH-1) (NADH: ubiquinone reductase), membraneanchored fumarate reductase/succinate dehydrogenase (SdhA, SdhB, SdhC), cytochrome bd complex (cydA and cydB), cydC subunit, which is required for the synthesis of the functional form of cytochrome bd, ATPase (atpA, atpB, atpC, atpD, atpE, atpF, atpG, atpH), and all genes indispensable for biosynthesis of heme, an essential cofactor for the cytochrome bd complex.…”

Section: Curation and Re Nement Of Central And Energy Metabolismmentioning

confidence: 99%

Genome Scale Constraint-Based Metabolic Reconstruction of Propionibacterium Freudenreichii DSM 20271

Borghei

Mobasheri²,

Sobati³

2021

Preprint

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Propionibacterium is an anaerobic bacterium with a history of use in the production of Swiss cheese and, more recently, several industrial bioproducts. While the use of this strain for the production of organic acids and secondary metabolites has gained growing interest, the industrial application of the strain requires further improvement in the yield and productivity of the target products. Systems modeling and analysis of metabolic networks are widely leveraged to gain holistic insights into the metabolic features of biotechnologically important strains and to devise metabolic engineering and culture optimization strategies for economically viable bioprocess development. In the present study, a high-quality genome-scale metabolic model of P. freudenreichii ssp. freudenreichii strain DSM 20271 was developed based on the strain’s genome annotation and biochemical and physiological data. The model covers the functions of 23% of the strain’s ORFs and accounts for 711 metabolic reactions and 647 unique metabolites. Literature-based reconstruction of the central metabolism and rigorous refinement of annotation data for establishing gene-protein-reaction associations renders the model a curated omic-scale knowledge base of the organism. Validation of the model against experimental data indicates that the reconstruction can capture the key structural and functional features of P. freudenreichii metabolism, including the growth rate, the pattern of flux distribution, the strain’s aerotolerance behavior, and the change in the mode of metabolic activity during the transition from an anaerobic to an aerobic growth regime. The model also includes an accurately curated pathway of cobalamin biosynthesis, which was used to examine the capacity of the strain to produce vitamin B12 precursors. Constraint-based reconstruction and analysis of the P. freudenreichii metabolic network also provided novel insights into the complexity and robustness of P. freudenreichii energy metabolism. The developed reconstruction, hence, may be used as a platform for the development of P. freudenreichii-based microbial cell factories and bioprocesses.

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“…The nutrient composition of the culture medium, such as amino acid or mineral composition including cobalt ions, affects production of vitamin B 12 . Two experimental findings led to major improvements in the production of vitamin B 12 : (a) addition of the precursor dimethylbenzimidazole, and (b) aerobic incubation in the latter phase of fermentation [32,51,52,60,76,77]. Moreover, an increase in these precursors or intermediary metabolites in the cells of the producer strain using genetic recombinant DNA technology will achieve overproduction of vitamin B 12 .…”

Section: Fermentation Of Cobalaminmentioning

confidence: 99%

Production of tetrapyrrole compounds and vitamin B12 using genetically engineering of Propionibacterium freudenreichii. An overview

Murooka

Piao

Kiatpapan

et al. 2005

Lait

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-Propionibacterium freudenreichii is a commercially important bacterium that is used in the production of cheeses, cobalamin (vitamin B 12 ) and propionic acid. Metabolic engineering using genetically improved strains will make the fermentation process more economical and also enhance the quality of the products. Host-vector systems and expression vectors using strong promoters from P. freudenreichii were developed in propionibacteria. By using these expression vectors and amplification of various genes, productions of 5-aminolevulinic acid, tetrapyrrole compounds and vitamin B 12 were reported. Here, we review the advancement of genetic engineering in P. freudenreichii in recent years, covering the molecular aspects of the formation of tetrapyrrole compounds and vitamin B12.

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Effect of the oxygen supply on pattern of growth and corrinoid and organic acid production of Propionibacterium shermanii

Cited by 11 publications

References 16 publications

Improved propionic acid production with metabolically engineered Propionibacterium jensenii by an oxidoreduction potential-shift control strategy

Improved propionic acid production with metabolically engineered Propionibacterium jensenii by an oxidoreduction potential-shift control strategy

Genome Scale Constraint-Based Metabolic Reconstruction of Propionibacterium Freudenreichii DSM 20271

Production of tetrapyrrole compounds and vitamin B12 using genetically engineering of Propionibacterium freudenreichii. An overview

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