Production of tetrapyrrole compounds 
and vitamin B12 using genetically engineering 
of <i>Propionibacterium freudenreichii. </i>An overview

Murooka, Yoshikatsu; Piao, Yongzhe; Kiatpapan, Pornpimon; Yamashita, Mitsuo

doi:10.1051/lait:2004035

Cited by 30 publications

(24 citation statements)

References 72 publications

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“…The pathway of vitamin B12 synthesis in Propionibacterium freudenreichii has been completely elucidated [40,102]. This microorganism synthesizes cobalamin as a cofactor for propionic acid fermentation [41] and is the only bacteria, among B12 producers that possess the GRAS status of the United States Food and Drug Administration.…”

Section: Nutraceuticals Production: Cla Vitamins Eps and Trehalosementioning

confidence: 99%

“…In consequence dairy propionibacteria are the preferred microorganisms for the industrial production of this vitamin and many efforts have been made to improve the production process by using genetic engineering [102,103] and other biotechnological strategies like fermentation manipulations [104,105].…”

Section: Nutraceuticals Production: Cla Vitamins Eps and Trehalosementioning

confidence: 99%

“…freudenreichii that overproduce B12 vitamin [102,107], porphyrin [108], and riboflavin (vitamin B2) [107].…”

Section: Nutraceuticals Production: Cla Vitamins Eps and Trehalosementioning

confidence: 99%

See 2 more Smart Citations

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Zárate¹

2012

Probiotic in Animals

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Section: Nutraceuticals Production: Cla Vitamins Eps and Trehalosementioning

confidence: 99%

Section: Nutraceuticals Production: Cla Vitamins Eps and Trehalosementioning

confidence: 99%

See 1 more Smart Citation

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Zárate¹

2012

Probiotic in Animals

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“…Since chemical synthesis is extremely complex, only biosynthetic processes are used for the commercial production of vitamin B 12 [27]. The microorganisms most widely used in these fermentations include Propionibacterium spp.…”

Section: Introductionmentioning

confidence: 99%

“…The microorganisms most widely used in these fermentations include Propionibacterium spp. [13,27], Pseudomonas denitrificans [21], methanogens (Methanosarcina barkeri, Methanobacterium spp.) and Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

Wyk

Britz

2010

Dairy Sci. Technol.

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-To overcome nutritional vitamin B 12 deficiencies in certain populations in Southern Africa, fortified functional foods have been developed. However, current microbiological methods used to accurately determine vitamin B 12 levels in foodstuffs, important for quality control and regulatory purposes, are time consuming. This study describes an extraction and detection method for vitamin B 12 in dairy products and growth media cultured with Propionibacterium freudenreichii. Samples were extracted by mixing in KCN buffer (pH 4.5), autoclaving at 121°C for 25 min, cooling and centrifugation. The resultant supernatant was syringe-filtered prior to reversed-phase HPLC analysis using a methanol-water gradient that was effective in resolving the B 12 peak. The method offered excellent linearity with a regression coefficient R > 0.998. The limit of quantification was 0.005 μg·mL −1 sample. For samples with vitamin B 12 concentrations well within the linear range of the assay, good repeatability was demonstrated for the same sample with mean concentrations of 2.62 ± 0.02 and 2.61 ± 0.02 μg·mL −1 detected on day 1 and day 2, respectively. Recovery values ranged from 98.6% to 103.2%, indicating that the extraction method ensured complete dissolution of vitamin B 12 from the matrices under study. Sensitivity was enhanced by sample concentration and purification using a series of solid phase extraction steps which resulted in improved peak resolution and removal of interfering peaks. The method was validated by comparison of the HPLC results of the same sample with those obtained using traditional microbiological methods. The method is a rapid alternative to the more time-consuming microbiological assay. Résumé -Une méthode HPLC rapide pour extraire et quantifier la vitamine B 12 dans les produits laitiers et les cultures de Propionibacterium freudenreichii. Pour lutter contre les carences en vitamine B 12 parmi certaines populations d'Afrique du Sud, des aliments fonctionnels enrichis ont été développés. Cependant, les méthodes actuelles utilisées pour analyser précisément le niveau de vitamine B 12 dans les aliments, qui sont importantes à des fins de contrôle-qualité et de législation, sont coûteuses en temps. Cet article décrit une méthode d'extraction et de détection de la vitamine B 12 dans les produits laitiers et les cultures de Propionibacterium freudenreichii. Les échantillons étaient extraits par mélange à du tampon KCN, pH 4,5 et autoclavage à 121°C pendant 25 min, refroidissement et centrifugation. Le surnageant résultant était filtré puis analysé par HPLC en phase inverse en utilisant un gradient méthanol-eau, qui permettait une bonne résolution du pic de vitamine B 12 . La méthode montrait une bonne linéarité, avec un coefficient de régression R > 0,998. La limite de quantification était de 0,005 μg·mL −1 d'échantillon. Pour des échantillons contenant des concentrations dans la moyenne de la gamme de linéarité, la répétabilité a été établie, avec des valeurs obtenues pour le même échantillon à deux jo...

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Metabolic profiling analysis of the vitamin B₁₂ producer Propionibacterium freudenreichii

Liu

et al. 2021

MicrobiologyOpen

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Vitamin B 12 (VB 12 ) is an indispensable cofactor of metabolic enzymes and has been widely used in the food and pharmaceutical industries. In this study, the effects of medium composition on VB 12 production by Propionibacterium freudenreichii were evaluated and optimized based on statistical experiments. The results showed that glucose, yeast extract, KH 2 PO 4 , and glycine have significant effects on VB 12 production. The final titer of VB 12 reached 8.32 ± 0.02 mg/L, representing a 120% increase over the non‐optimized culture medium. We employed a metabolomics approach to analyze the differences of metabolite concentrations in P . freudenreichii cells cultivated in the original medium and optimized fermentation medium. Using multivariate data analysis, we identified a range of correlated metabolites, illustrating how metabolomics can be used to explain VB 12 production changes by corresponding differences in the overall cellular metabolism. The concentrations of many metabolic intermediates of glycolysis, the Wood–Werkman cycle, the TCA cycle, and amino acid metabolism were increased, which contributed to the synthesis of propionic acid and VB 12 due to an improved supply of energy and precursors.

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Production of tetrapyrrole compounds and vitamin B12 using genetically engineering of Propionibacterium freudenreichii. An overview

Cited by 30 publications

References 72 publications

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

Metabolic profiling analysis of the vitamin B₁₂ producer Propionibacterium freudenreichii

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Production of tetrapyrrole compounds and vitamin B12 using genetically engineering of Propionibacterium freudenreichii. An overview

Cited by 30 publications

References 72 publications

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

A rapid HPLC method for the extraction and quantification of vitamin B12in dairy products and cultures ofPropionibacterium freudenreichii

Metabolic profiling analysis of the vitamin B12 producer Propionibacterium freudenreichii

Contact Info

Product

Resources

About

A rapid HPLC method for the extraction and quantification of vitamin B₁₂in dairy products and cultures ofPropionibacterium freudenreichii

Metabolic profiling analysis of the vitamin B₁₂ producer Propionibacterium freudenreichii