Effect of the osteoplastic material processing methods on the remodeling in the experimental bone defect model in vivo

Vorobyov, Konstantin; Божкова, С. А.; Anisimova, Lyudmila; Нетылько, Г. И.

doi:10.32000/2072-1757-2019-1-67-72

Cited by 2 publications

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“…В настоящее время увеличивается потребность в костнопластических материалах для оказания высокотехнологичной медицинской помощи в травматологии, ортопедии и челюстно-лицевой хирургии пациентам, нуждающимся в замещении костных дефектов и восполнении участков атрофии костной ткани разного этиопатогенеза [1,2,3,4]. Характер регенеративных процессов костной ткани при замещении костного дефекта в значительной мере определяется свойствами самого костнозамещающего материала [1,3,5]. Существующие искусственные костнозамещающие материалы не обладают необходимыми характеристиками, в полной мере соответствующими свойствам костной ткани [1,6,7].…”

Section: Introductionunclassified

“…Характер регенеративных процессов костной ткани при замещении костного дефекта в значительной мере определяется свойствами самого костнозамещающего материала [1,3,5]. Существующие искусственные костнозамещающие материалы не обладают необходимыми характеристиками, в полной мере соответствующими свойствам костной ткани [1,6,7]. Несмотря на то, что «золотым стандартом» при замещении патологически измененных участков костной ткани в рамках рекон-структивно-пластического хирургического лечения является аутотрансплантат, в качестве альтернативы, оптимально соответствующей по своим структурно-функциональным характеристикам аутокости, применяются аллотрансплантаты [7,8,9,10].…”

Section: Introductionunclassified

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In Vitro Evaluation of the Allogeneic Bone Matrix Effect on the Adipose Mesenchymal Stromal Cells Characteristics in Combined Tissue Engineering

Черданцева

Anastasieva

Алейник

et al. 2021

Traumatology and Orthopedics of Russia

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The aim of the study was to evaluate in vitro the effect of native and deproteinized compact and spongy allogenic bone matrices on the characteristics of adipose mesenchymal stromal cells (ASC) in combined tissue engineering.Material and Methods. 24 samples of native and deproteinized compact and spongy bone were examined, which were exposed to mechanical treatment, modeling, followed by sterilization of the samples by ionizing radiation and bacteriological control of sterilization. Some of the samples underwent deproteinization. The characterized cultures of human ASC were used as test cultures to assess the interaction with the bone samples. The Cytation-5 fluorescent imager and Hoechst 3334 fluorochromes (BD Pharmingen™) and calcein (Calcein AM, BD Pharmingen™) were used to characterize the degree of adhesion, migration, and viability of ASC on bone matrix samples. Matrix cytotoxicity was evaluated by MTT assay on days 1 and 7 of extraction.Results. The bone matrix samples are characterized by the absence of cytotoxicity (rank 1). ASC demonstrated good adhesion and migration on any surface of the bone matrix and preservation of cell viability during 7 days of observation. Nuclei sizes of the cells adhered to the deproteinized bone matrix of the spongy structure increased by 25–30% compared to other samples. The cells on deproteinized bone matrix had greater size (the size of the cells from nuclei 8.8 to 11.5 μm, the average size of cells nuclei from an 86.3 μm to 129,0 μm, the average perimeter of the cells nuclei from 30.7 μm to 40.7 μm) than in the native bone matrix samples.Conclusion. The results of the study of various allogeneic bone matrices demonstrate that deep purification of the bone matrix determines the absence of cytotoxicity and the most favorable conditions for the adhesion, migration, proliferation and viability of ASC. Also makes it possible to use tissue engineering based on bone matrices of different structures. Deproteinized spongy bone matrices are best suited for this purpose.

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Section: Introductionunclassified

In Vitro Evaluation of the Allogeneic Bone Matrix Effect on the Adipose Mesenchymal Stromal Cells Characteristics in Combined Tissue Engineering

Черданцева

Anastasieva

Алейник

et al. 2021

Traumatology and Orthopedics of Russia

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Deproteinized Bone Tissue as a Matrix for Tissue-Engineered Construction: Experimental Study

Anastasieva

Черданцева

Толстикова

et al. 2023

Traumatology and Orthopedics of Russia

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Background. At present, for a number of reasons the complete bone defect replacement with autogenous bone is not always possible. Bone substitute materials are used as an alternative to autogenous bone tissue and can be of either biological or non-biological origin. One of the ways of development of reconstructive technologies is the use of tissue-engineered constructs that fully imitate autogenous bone tissue in the required volume. Aim of study to define in vivo the possibility of using deproteinized human cancellous bone tissue as a matrix for creating tissue-engineered constructs. Methods. An in vivo study was carried out on NZW rabbits. To create a construct, we used the fragments of deproteinized cancellous bone tissue of the human femoral head and stromal vascular fraction of rabbit adipose tissue as a matrix. Bone defect modeling with its subsequent replacement was performed to evaluate the efficacy of reparative osteogenesis during bone defects reconstruction. Study groups were defined: group 1 (control) surgical modeling of a bone defect of the femur without its reconstruction; group 2 surgical modeling of a bone defect of the femur with its reconstruction using fragments of deproteinized cancellous bone matrix; group 3 surgical modeling of a bone defect of the femur with its reconstruction using fragments of deproteinized cancellous bone matrix in combination with stromal vascular fraction of adipose tissue (according to ACP SVF technology). Results. Comparative analysis of reparative processes in case of applying tissue-engineered constructs based on deproteinized human cancellous bone matrix in combination with adipose tissue-derived stromal vascular fraction on in vivo experimental model revealed that the use of these bone substitute materials contributes not only to an early activation of reparative regeneration of main structural elements of the bone tissue in the area of the bone defect replacement, but also to its well-timed differentiation. This determines the restoration of structural and functional viability of the bone tissue at the damage site without developing discernible reactive inflammation. Moreover, the effect of the selected tissue-engineered construct with the combined influence of several factors (ACP SVF) in its composition turned out to be more effective in stimulating bone tissue repair and differentiation. Conclusion. Combination of SVF and deproteinized bone matrix for creating tissue-engineered constructs enables to engage several regeneration mechanisms and accelerate the process of bone defect replacement in comparison with isolated deproteinized bone matrix without bone defect reconstruction.

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Effect of the osteoplastic material processing methods on the remodeling in the experimental bone defect model in vivo

Cited by 2 publications

References 0 publications

In Vitro Evaluation of the Allogeneic Bone Matrix Effect on the Adipose Mesenchymal Stromal Cells Characteristics in Combined Tissue Engineering

In Vitro Evaluation of the Allogeneic Bone Matrix Effect on the Adipose Mesenchymal Stromal Cells Characteristics in Combined Tissue Engineering

Deproteinized Bone Tissue as a Matrix for Tissue-Engineered Construction: Experimental Study

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