Effect of the matrix structure and concentration of polymer-immobilized Ni2+–iminodiacetic acid complexes on retention of IgG1

Kráčalíková, Kateřina; Tishchenko, Galina; Bleha, M.

doi:10.1016/j.jbbm.2005.04.010

Cited by 11 publications

(1 citation statement)

References 41 publications

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“…The selection of functional groups was considered from various perspectives, and the functional group used in this experiment was determined to be disodium iminodiacetate monohydrate (IDA). The IDA group is a functional group [31][32][33][34][35] widely used in protein adsorption, enzyme purification and fixation, and metal ion adsorption. In particular, IDA is judged to be more efficient when introducing sialic acid because there are two places where sialic acid can be introduced.…”

Section: Mimic Of Sugar Cluster Effect Of Functional Mnwfmentioning

confidence: 99%

Functional Microfiber Nonwoven Fabric with Sialic Acid-Immobilized Polymer Brush for Capturing Lectin in Aerosol

2022

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The influenza virus has been known as a representative infectious virus that harms human health from the past to the present day. We have promoted the development of a novel adsorbent capable of adsorbing influenza viruses in the form of aerosols in the air. In this study, to develop a material to adsorb the influenza virus, a functional group was introduced into a microfiber nonwoven fabric (MNWF) manufactured through radiation-induced graft polymerization (RIGP), and sialic acid was immobilized to mimic the sugar chain cluster effect. The functional group was used by coupling disodium iminodiacetate monohydrate (IDA) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and N-acetylneuraminic acid (NANA) was selected for sialic acid. IDA-EDC was introduced into GMA MNWF with an average molar conversion of 47%. For NANA MNWF with a degree of grafting (dg) of 87% introduced with sialic acid, 118.2 of 200 µg of aerosolized lectin was adsorbed, confirming that the maximum adsorption amount was 59.1%. In NANA MNWF of 100% or more dg, a tendency to decrease the amount of lectin adsorption was observed compared to NANA MNWF of 80–100% dg.

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Section: Mimic Of Sugar Cluster Effect Of Functional Mnwfmentioning

confidence: 99%

Functional Microfiber Nonwoven Fabric with Sialic Acid-Immobilized Polymer Brush for Capturing Lectin in Aerosol

2022

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Synthesis and modification of supports with an alkylamine and their use in albumin adsorption

Gómez

Strumia

2008

J. Polym. Sci. A Polym. Chem.

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The morphological effect of polymeric networks (R) modified with terminal amino groups was studied on the adsorption of bovine serum albumin (BSA). Networks of ethylene glycol dimethacrylate and 2‐hydroxyethyl methacrylate [poly (EGDMA‐co‐HEMA)] were synthesized by suspension polymerization, using different EGDMA contents and agitation speeds. These matrices were characterized by FTIR, mercury intrusion porosimetry, SEM, and swelling degree. The increase of the EGDMA concentration led to the formation of networks with the highest crosslinking degree and porosity. An earlier phase separation yielded a higher aggregation of rigid microspheres, also forming stable pore systems. The increase in coalescence frequency, together with the impeller speed, and the decrease of the stabilizer molecules led to an increment in drop size. Large fused aggregates of microspheres were formed with additional loss of small pores as the stirring was increased, attaining also a higher pore volume (Vp) and a slight decrease of the surface area. Once characterized, networks were activated with butanediolglycidyl ether (BDGE), and then reacted with hexamethylenediamine (HMDA) through coupling reaction. Only the R‐BDGE‐HMDA networks synthesized with the highest EGDMA content and agitation speed showed BSA adsorption. Their base matrices exhibited a Vp higher than 1.4 mL/g, which allows easier protein diffusion into the support. © 2008 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 2557–2566, 2008

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The effect of NaCl on the adsorption of human IgG onto CM-Asp–PEVA hollow fiber membrane-immobilized nickel and cobalt metal ions

et al. 2014

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Effect of the matrix structure and concentration of polymer-immobilized Ni2+–iminodiacetic acid complexes on retention of IgG1

Cited by 11 publications

References 41 publications

Functional Microfiber Nonwoven Fabric with Sialic Acid-Immobilized Polymer Brush for Capturing Lectin in Aerosol

Functional Microfiber Nonwoven Fabric with Sialic Acid-Immobilized Polymer Brush for Capturing Lectin in Aerosol

Synthesis and modification of supports with an alkylamine and their use in albumin adsorption

The effect of NaCl on the adsorption of human IgG onto CM-Asp–PEVA hollow fiber membrane-immobilized nickel and cobalt metal ions

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