Effect of the ionic product of bioglass 60s on osteoblastic activity in canines

Alves, Endrigo Gabellini Leonel; Serákides, Rogéria; Rosado, Isabel Rodrigues; Pereira, Marivalda M.; Ocarino, Natália de Melo; Oliveira, H.P.; Góes, Alfredo M.; Rezende, C.M.F.

doi:10.1186/s12917-015-0558-7

Cited by 10 publications

(9 citation statements)

References 19 publications

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“…Even the observation of BG agglomerations and giant cell formation has been reported elsewhere [29,30]. There is evidence that BG has direct osteogenic and mineralogenic effects in vitro through the promotion of ALP activity and the enhanced expression of osteogenic genes such as osteocalcin, RUNX2, BMP2, BMP6, osterix, bone sialoprotein, osteonectin and collagen I [36][37][38][39], yet, while bone induction in an ectopic intramuscular site has been previously reported by BG generated glass-ceramic scaffolds [40], no previous study has reported BG-induced bone formation in a subcutaneous site without the addition of osteogenic cells (despite implantation times ranging from 7 to 60 days).…”

Section: In Vivo Mineralizationmentioning

confidence: 99%

Ectopic bone formation in rapidly fabricated acellular injectable dense collagen-Bioglass hybrid scaffolds via gel aspiration-ejection

et al. 2016

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Gel aspiration-ejection (GAE) has recently been introduced as an effective technique for the rapid production of injectable dense collagen (IDC) gel scaffolds with tunable collagen fibrillar densities (CFDs) and microstructures. Herein, a GAE system was applied for the advanced production and delivery of IDC and IDC-Bioglass(®) (IDC-BG) hybrid gel scaffolds for potential bone tissue engineering applications. The efficacy of GAE in generating mineralizable IDC-BG gels (from an initial 75-25 collagen-BG ratio) produced through needle gauge numbers 8G (3.4 mm diameter and 6 wt% CFD) and 14G (1.6 mm diameter and 14 wt% CFD) was investigated. Second harmonic generation (SHG) imaging of as-made gels revealed an increase in collagen fibril alignment with needle gauge number. In vitro mineralization of IDC-BG gels was confirmed where carbonated hydroxyapatite was detected as early as day 1 in simulated body fluid, which progressively increased up to day 14. In vivo mineralization of, and host response to, acellular IDC and IDC-BG gel scaffolds were further investigated following subcutaneous injection in adult rats. Mineralization, neovascularization and cell infiltration into the scaffolds was enhanced by the addition of BG and at day 21 post injection, there was evidence of remodelling of granulation tissue into woven bone-like tissue in IDC-BG. SHG imaging of explanted scaffolds indicated collagen fibril remodelling through cell infiltration and mineralization over time. In sum, the results suggest that IDC-BG hybrid gels have osteoinductive properties and potentially offer a novel therapeutic approach for procedures requiring the injectable delivery of a malleable and dynamic bone graft that mineralizes under physiological conditions.

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Section: In Vivo Mineralizationmentioning

confidence: 99%

Ectopic bone formation in rapidly fabricated acellular injectable dense collagen-Bioglass hybrid scaffolds via gel aspiration-ejection

et al. 2016

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“…To date, little information is available concerning the effects of BGs on the osteogenic differentiation of MSCs in vitro (11,(38)(39)(40). In a study by Reilly et al, no significant difference in levels of alkaline phosphatase activity was seen between human MSCs grown in the presence of 45S5 BG or tissue culture plastic, hence they reported that positive effect of BG on bone growth is not correlated with the accelerated differentiation of MSC.…”

Section: Introductionmentioning

confidence: 99%

Influence of calcium and phosphorus release from bioactive glasses on viability and differentiation of dental pulp stem cells

et al. 2017

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The release of ions that can significantly contribute toward cellular response is an important characteristic of bioactive glasses (BG). Here, ionic extracts of three different compositions of BG powders in 60 mol% SiO2, x mol% CaO (x = 28, 32 and 36), x mol% P2O5 (x = 12, 8 and 4) compositional system were utilized to study their effect on the viability, differentiation and mineralization of dental pulp stem cells (DPSCs) in vitro. ICP was applied to detect the exact ionic concentrations released from different composition of BG. DPSCs treated with conditioned media from the glass with 4 mol% P2O5 (BGCM1, media containing 44.01 ± 0.6 mg/L Si, 61.72 ± 0.1 mg/L Ca and 7.57 ± 0.01 mg/L P) were more metabolically active compared to conditioned media from the glass with 8 mol% P2O5 (BGCM2, media with 47.36 ± 0.7 mg/L Si, 57.4 ± 0.1 mg/L Ca and 14.54 ± 0.2 mg/L P), at all times tested, but in all cases the process was slower than the control. Cells exposed to media conditioned by the glass with 12 mol% P2O5 (BGCM3, 40.46 ± 0.5 mg/L Si, 61. 85 ± 0.3 mg/L Ca and 28.43 ± 0.3 mg/L P) responded differently, such that cells showed to be more metabolically active than control at day 3, but then similar to or lower than control at higher time points. Differentiation of DPSCs toward osteogenic lineage in the presence of BGCM was assessed by Alizarin red staining. Cells treated with high phosphate BGCM3 displayed a higher density of red mineralized nodules than cells treated with BGCM1 and BGCM2 after 21 days of culture in non-osteogenic medium. BGCM3 was therefore chosen for gene expression studies. Osteogenic differentiation of DPSCs in the presence and/or absence of BGCM3 or osteogenic supplements were studied by RT-PCR. Overall, the results demonstrated that, in the absence of osteogenic supplements, BGCM3 group showed a significantly higher mRNA expression levels for alkaline phosphatase at day 7, osteopontin and osteonectin at days 7 and 14, and a high level of collagen I at day 14, compared to negative control group (BM−). Overall, the results obtained from BGCM3 group are beneficial for the design and manufacture of scaffolds or particulates with tailored ion release for a range of bone repair applications

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“…In addition, recent studies reported that ions can act in pairs to generate combinational effects superior to the sum of the individual ionic contribution on cell metabolism [15][16][17][18]. For instance, when calcium ions and silica were both present in the culture of mouse pre-osteoblasts, an increase in the expression of osteocalcin was observed [15], which is a biomarker for bone formation.…”

Section: Introductionmentioning

confidence: 99%

Lithium-silicate sol–gel bioactive glass and the effect of lithium precursor on structure–property relationships

Maçon

Jacquemin

Page

et al. 2016

J Sol-Gel Sci Technol

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This work reports the synthesis of lithium-silicate glass, containing 10 mol% of Li 2 O by the sol-gel process, intended for the regeneration of cartilage. Lithium citrate and lithium nitrate were selected as lithium precursors. The effects of the lithium precursor on the sol-gel process, and the resulting glass structure, morphology, dissolution behaviour, chondrocyte viability and proliferation, were investigated. When lithium citrate was used, mesoporous glass containing lithium as a network modifier was obtained, whereas the use of lithium nitrate produced relatively dense glass-ceramic with the presence of lithium metasilicate, as shown by X-ray diffraction, 29 Si and 7 Li MAS NMR and nitrogen sorption data. Nitrate has a better affinity for lithium than citrate, leading to heterogeneous crystallisation from the mesopores, where lithium salts precipitated during drying. Citrate decomposed at a lower temperature, where the crystallisation of lithium-silicate crystal is not thermodynamically favourable. Upon decomposition of the citrate, a solid-state salt metathesis reaction between citrate and silanol occurred, followed by the diffusion of lithium within the structure of the glass. Both glass and glass-ceramic released silica and lithium ions in culture media, but release rate was lower for the glass-ceramic. Both samples did not affect chondrocyte viability and proliferation.Graphical Abstract

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Effect of the ionic product of bioglass 60s on osteoblastic activity in canines

Cited by 10 publications

References 19 publications

Ectopic bone formation in rapidly fabricated acellular injectable dense collagen-Bioglass hybrid scaffolds via gel aspiration-ejection

Ectopic bone formation in rapidly fabricated acellular injectable dense collagen-Bioglass hybrid scaffolds via gel aspiration-ejection

Influence of calcium and phosphorus release from bioactive glasses on viability and differentiation of dental pulp stem cells

Lithium-silicate sol–gel bioactive glass and the effect of lithium precursor on structure–property relationships

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