Effect of the fat composition of a single high-fat meal on inflammatory markers in healthy young women

Abstract

The aim of the present study was to examine the effect of a single high-fat meal with different fat quality on circulating inflammatory markers and gene expression in peripheral blood mononuclear cells (PBMC) to elucidate the role of fat quality on postprandial inflammation. A postprandial study with fourteen healthy females consuming three test meals with different fat quality was performed. Test days were separated by 2 weeks. Fasting and postprandial blood samples at 3 and 6 h after intake were analysed. The test meal consisted of three cakes enriched with coconut fat (43 % energy as saturated fat and 1 % energy as a-linolenic acid (ALA)), linseed oil (14 % energy as ALA and 30 % energy as saturated fat) and cod liver oil (5 % energy as EPA and DHA and 5 % energy as ALA in addition to 31 % energy as saturated fat). In addition, ex vivo PBMC experiments were performed in eight healthy subjects investigating the effects of EPA and ALA on release and gene expression of inflammatory markers. The IL-8 mRNA level was significantly increased after intake of the cod liver oil cake at 6 h compared with fasting level, which was significantly different from the effect observed after the intake of linseed cake. In contrast, no effect was seen on circulating level of IL-8. In addition, ALA and EPA were shown to elicit different effects on the release and mRNA expression levels of inflammatory markers in PBMC cultured ex vivo, with EPA having the most prominent proinflammatory potential.

Marine n-3 Fatty Acids and Gene Expression in Peripheral Blood Mononuclear Cells

“…We have also performed a postprandial study where 3 different test meals were consumed by 14 healthy women in a fixed order with 14 days wash-out between each test meal [26•]. The test meals consisted of 3 different 150 g chocolate cakes enriched with coconut fat [43 % energy as saturated fat and 1 % energy as alpha-linolenic acid (ALA)], linseed oil (14 % energy as ALA and 30 % energy as saturated fat), and cod liver oil (5 % energy as EPA and DHA and 5 % energy as ALA in addition to 31 % energy as saturated fat).…”

Cod liver oil in sodium nitrite induced hepatic injury: Does it have a potential protective effect?

“…7 Cod liver oil has many favorable biological properties such as antiinflammatory, antioxidant, and antifibrosis properties. [8][9][10] Recent understanding of the molecular events of increased levels of sodium nitrite has focused researchers' attention on oxidative stress in different body organs. 8,11 Less attention has been paid to other effects like inflammation.…”

Portal vein cytokines in the early phase of acute experimental oedematous and necrotizing porcine pancreatitis

“…Catalog # M500KCAFOY, CA, US) and Milliplex Human Cytokine/Chemokine Magnetic Panel (Millipore, Catalog # MPXHCYTOMAG-60K, Millipore Corporation, Billerica MA, US) utilizing a Bio-Plex instrument based on Luminex xMAP Technology (Bio-Rad Laboratories Inc., CA, US). The measurement was performed according to manufacturer's instructions, as previously described [11]. Effect of the fat composition of a single high-fat meal on inflammatory markers in healthy young women).…”