Effect of the Cytoskeletal Fixation Agent Phalloidin on Transcapillary Albumin Transport and Interstitial Fluid Pressure in Anaphylaxis in the Wistar Rat

Abstract

This study shows that pretreatment with phalloidin, before the administration of dextran, abolishes the lowering of P(if) and edema formation, which is detected after i.v. injection of dextran alone.

Effect of the cytoskeletal fixation agent phalloidin on transcapillary albumin transport and interstitial fluid pressure following subdermal prostaglandin E1 administration in the rat

“…One could argue that a dramatic drop in arterial blood pressure, caused by phalloidin, could prevent oedema formation by lowering the hydrostatic gradients over the capillary barrier. However, no such changes in arterial blood pressure were demonstrated (Bronstad et al 2002).…”

“…In another study using phalloidin we have shown that the animals treated with phalloidin showed no significant alterations in arterial blood pressure (Bronstad et al 2002). One could argue that a dramatic drop in arterial blood pressure, caused by phalloidin, could prevent oedema formation by lowering the hydrostatic gradients over the capillary barrier.…”

“…PGE 1 was dissolved in sterile saline to a concentration of 0.85 mg mL )1 . The dosages of the different test substances were selected based on earlier experiments related to pro-and anti-inflammatory properties of the substances (Asako et al 1992, Berg et al 1998, Bronstad et al 2002. Human serum albumin (HSA), labelled with 125 I or 131 I were supplied by Institute for Energy Technique (Kjeller, Norway).…”

“…P if was measured by micropuncture technique using micropipettes connected to a servo-controlled counterpressure device. The method has earlier been validated and criteria for acceptance of the measurement have been defined elsewhere (Wiig et al 1981, Bronstad et al 2002. Pipettes were made from glass capillaries (GC 1015, 1.00 mm OD · 0.58 mm ID).…”

“…This hypothesis was strongly supported by in vivo experiments using cytochalasin-D, an actin filament-disrupting agent, which caused lowering of P if concomitant with oedema formation (Berg et al 2001). Furthermore, pre-treatment with phalloidin, a potent actin filament stabilizer effectively prevented lowering of P if and attenuated the oedema formation induced by mast cell degranulation in the rat (Bronstad et al 2002).…”