New and active role of the interstitium in control of interstitial fluid pressure: potential therapeutic consequences

Wiig, Helge; Rubin, Kristofer; Reed, Rolf K.

doi:10.1034/j.1399-6576.2003.00050.x

Cited by 137 publications

(122 citation statements)

References 61 publications

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“…Cultured human dermal fibroblasts will compact a gel to 10% of the initial gel area in 12-24 h. The process of collagen gel compaction depends on "1-integrins (Gullberg et al 1990) and on intercellular adhesions and Gap-junctions synchronizing the work (Ehrlich et al 2000). Several studies from our laboratory have shown that the characteristics of the collagen gel compaction mimic the cellular control of interstitial fluid pressure in vivo (Wiig et al 2003;Ahlen et al 1998;Berg et al 1998;Heuchel et al 1999). We found that the human explant ED/ES fibroblasts compacted collagen gels to the same degree and with the same kinetics as dermal fibroblasts.…”

Section: Discussionmentioning

confidence: 97%

“…Interstitial CT cells in dermis and trachea actively modulate interstitial fluid pressure via forces generated by cell-ECM interactions (Reed et al 1992;Rodt et al 1996) and the actin cytoskeleton (Berg et al 2001;Bronstad et al 2002). This cellular contractility restricts the intrinsic swelling tendency of the glycosaminoglycan-rich ground substance (Wiig et al 2003). Accordingly, it is plausible that cell-ECM contacts of periductal CT cells are important for the control of interstitial fluid pressure in the inner ear.…”

Section: Discussionmentioning

confidence: 99%

“…According to new concepts in CT physiology, fibroblasts of the CT control the interstitial fluid pressure and tissue volume via actively regulated intercellular adhesion and adhesion to fibers of the ECM, thereby restraining the intrinsic tendency of glycosaminoglycans to bind water and expand (Wiig et al 2003). Platelet derived growth factor-BB (PDGF-BB) is a fibroblast mitogen and promigratory factor acting via tyrosine kinase (PDGF-!…”

Section: Introductionmentioning

confidence: 99%

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An Interstitial Network of Podoplanin-Expressing Cells in the Human Endolymphatic Duct

Ekwall

Mayerl

Rubin

et al. 2006

JARO

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The human endolymphatic duct (ED) with encompassing interstitial connective tissue (CT) is believed to be important for endolymph resorption and fluid pressure regulation of the inner ear. The periductal CT cells are interconnected via numerous cellular extensions, but do not form vessel structures. Here we report that the periductal CT is populated by two distinct cell phenotypes; one expressing podoplanin, a protein otherwise found on lymph endothelia and on epithelia involved in fluid fluxes, and a second expressing a fibroblast marker. A majority of the interstitial cells expressed podoplanin but not the lymphatic endothelial cell markers hyaluronan receptor (LYVE-1) or vascular endothelial growth factor receptor-3 (VEGFR-3). The fibroblast marker positive cells were found close to the ED epithelium. In the mid-and distal parts of the ED, these cells were enriched under folded epithelia. Furthermore, subepithelial CT cells were found to express activated platelet derived growth factor (PDGF)-" receptors. Cultured CT cells from human inner ear periductal and perisaccular explant tissues were identified as fibroblasts. These cells compacted a three-dimensional collagen lattice by a process that could be promoted by PDGF-BB, a factor involved in interstitial fluid pressure regulation. Our results are compatible with the notion that the periductal CT cells are involved in the regulation of inner ear fluid pressure. By active compaction of the periductal CT and by the formation of villous structures, the CT cells could modulate fluid fluxes over the ED epithelium as well as the longitudinal flow of endolymph in the ED.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An Interstitial Network of Podoplanin-Expressing Cells in the Human Endolymphatic Duct

Ekwall

Mayerl

Rubin

et al. 2006

JARO

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“…It has been estimated that each cell can synthesise approximately 3.5 million procollagen molecules per day (McAnulty et al 1991). Fibroblasts also play an important role in regulating tissue interstitial fluid volume and pressure (Wiig et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Biological and morphological characterization of human neonatal fibroblast cell culture B-HNF-1

et al. 2010

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In the present study, human neonatal fibroblasts were isolated from a two-month-old human male. The purpose of the present investigation was the analysis of the morphology (light and transmission electron microscopy), karyotype and growth characteristics of the human neonatal fibroblast cell culture B-HNF-1. Moreover, STR typing and mitochondrial DNA amplification and sequencing was also performed. Analysis of chromosomes count showed that B-HNF-1 cell culture is diploid and has normal male karyotype 46, XY, which was stable during cultivation. The transmission electron microscopy demonstrated the ultra-structure of the B-HNF-1 cells; they have typical morphological features of proteosynthesis-active cells. Large number of fibroblasts bearing different shapes and surface characteristics adhered to the substrate with microvilli and filopodia. Our in vitro expanded fibroblasts have a large and irregular nucleus with prevalence of euchromatin. One to three nucleoli are present in each nucleus. The cytoplasm contains a richly developed rough endoplasmic reticulum and prominent Golgi apparatus cisterns. The result of the fragment analysis is a DNA profile defined as multiplex of STR markers and sex determining system. Sequencing analysis of hypervariable segments of control region of mitochondrial DNA showed haplotype that belongs to haplogroup W. In this study, we complexly characterized a new cell culture of human neonatal fibroblasts B-HNF-1 from different points of view. This culture should be used for further biomedical experiments.

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“…As a result, the tissue is in a sense tunable according to the mechanical needs of the body. Such tunability or matrix remodeling has been implicated in diverse aspects of physiology including control of interstitial fluid pressure [2], aging [3], repair [4][5][6], fibrosis [7,8] and tumorigenesis [9]. Not surprisingly, matrix remodeling also is an important consideration for tissue engineering [10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Fibroblast mechanics in 3D collagen matrices☆

Rhee

Grinnell²

2007

Advanced Drug Delivery Reviews

166

115

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Connective tissues provide mechanical support and frameworks for the other tissues of the body. Type 1 collagen is the major protein component of ordinary connective tissue, and fibroblasts are the cell type primarily responsible for its biosynthesis and remodeling. Research on fibroblasts interacting with collagen matrices explores all four quadrants of cell mechanics: pro-migratory vs. pro-contractile growth factor environments on one axis; high tension vs. low tension cell-matrix interactions on the other. The dendritic fibroblast -probably equivalent to the resting tissue fibroblast -can be observed only in the low tension quadrant and generally has not been appreciated from research on cells incubated with planar culture surfaces. Fibroblasts in the low tension quadrant require microtubules for formation of dendritic extensions, whereas fibroblasts in the high tension quadrant require microtubules for polarization but not for spreading. Ruffling of dendritic extensions rather than their overall protrusion or retraction provides the mechanism for remodeling of floating collagen matrices, and floating matrix remodeling likely reflects a model of tissue mechanical homeostasis.

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New and active role of the interstitium in control of interstitial fluid pressure: potential therapeutic consequences

Cited by 137 publications

References 61 publications

An Interstitial Network of Podoplanin-Expressing Cells in the Human Endolymphatic Duct

An Interstitial Network of Podoplanin-Expressing Cells in the Human Endolymphatic Duct

Biological and morphological characterization of human neonatal fibroblast cell culture B-HNF-1

Fibroblast mechanics in 3D collagen matrices☆

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