Effect of the Combination of Sodium Hypochlorite and Chlorhexidine on Dentinal Permeability and Scanning Electron Microscopy Precipitate Observation

Akisue, Eduardo; Tomita, Viviane S.; Gavini, Giulio; Figueiredo, José Antônio Poli de

doi:10.1016/j.joen.2009.11.019

Cited by 80 publications

(116 citation statements)

References 26 publications

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“…This precipitate forms a chemical smear layer (CSL) on dentin surface, occluding the dentinal tubules. This layer may affect close adaptation between root canal filling materials and the root canal walls, interfering in the seal of the root canal filling (8,9). In addition, this precipitate changes the color of the tooth and is cytotoxic (10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Effect of Intermediate Flush Using Different Devices to Prevent Chemical Smear Layer Formation

et al. 2017

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This study compared the effect of intermediate flush with distilled water delivered by conventional irrigation, EndoVac microcannula or Self-Adjusting File (SAF) system in the prevention of chemical smear layer (CSL) formation. Thirty human premolars were used. Canals were prepared with Reciproc system and 5.25% NaOCl. After chemomechanical preparation, samples were divided in 3 groups (n=10) according to the intermediate irrigation protocol with distilled water using: conventional irrigation, EndoVac microcannula or SAF. A final flush with 2% chlorhexidine solution was used and scanning electron microscopy was performed to assess protocol effectiveness. Two calibrated evaluators attributed scores according the presence or absence of CSL on the surface of the root canal walls at the coronal, middle and apical thirds, as follows: (1) no CSL; (2) small amounts of CSL; (3) moderate CSL; and (4) heavy CSL. Differences between protocols were analyzed with Kruskal-Wallis and Mann-Whitney U tests. Friedman and Wilcoxon signed rank tests were used for comparison between each root canal third. SAF resulted in less formation of CSL when compared with the conventional irrigation and EndoVac microcannula (p<0.05). When root canal thirds were analyzed, conventional irrigation and EndoVac groups showed less CSL formation at coronal and middle thirds in comparison to the apical third (p<0.05). In SAF group, there was no difference among the thirds (p>0.05). It may be concluded that an intermediate flush of distilled water, delivered by the SAF system resulted in a better reduction of CSL formation during chemomechanical preparation.

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Section: Introductionmentioning

confidence: 99%

Effect of Intermediate Flush Using Different Devices to Prevent Chemical Smear Layer Formation

et al. 2017

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“…Previous studies have shown the formation of an orange-brown precipitate when CHX is used in combination with NaOCl (126)(127)(128)(129). This solid precipitate produced may occlude the dentinal tubules and form a barrier between the filling material and dentin surface and therefore increase the risk of microleakage (127,130).…”

Section: Chlorhexidinementioning

confidence: 99%

Bioactive Chitosan Nanoparticles and Photodynamic Therapy Inhibit Collagen Degradation In Vitro

Persadmehr

Torneck

Cvitkovitch

et al. 2014

Journal of Endodontics

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This study evaluated the ability of photodynamic therapy (PDT), chitosan nanoparticles (CSnp), or their combination, to inhibit bacterial collagenase-mediated degradation of collagen. Rat type 1 fibrillar collagen matrices were untreated or treated with 2.5% glutaraldehyde (GD), 2.5% GD followed by 1% CSnp, 1% CSnp, PDT, or 1% CSnp followed by PDT. Samples, except untreated controls, were exposed to Clostridium histolyticum collagenase. The soluble digestion products were assessed by hydroxyproline assay and the remaining adherent collagen was quantified by picrosirius red (PSR) staining. Collagen treated with CSnp, PDT, or a combination of CSnp and PDT, exhibited less degradation than controls. The abundance of post-treatment residual collagen correlated with the extent of degradation. Fourier transform infrared (FTIR) spectroscopy analysis showed that PDT treatment enhanced collagen cross-linking.Immunoblotting of sedimented CSnp indicated that CSnp and collagenase bound with low affinity. However, CSnp-bound collagenase showed a significant reduction in collagenolytic activity compared with controls.III

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“…If contact occurs accidentally between CHX and NaOCl during irrigation, the formation of an orange-brown residue and the formation of a chemical smear layer occurs, which may exhibit cytotoxic potential (49,51), block the dentinal tubules, impair adhesion of material for the definite obturation (73,74), and cause a colour change of dentin (75)(76)(77). When CHX comes into contact with EDTA, the formation of a milky white precipitate occurs as a result of an acid-base reaction (49).…”

Section: Chlorhexidine Chxmentioning

confidence: 99%

Consideration of the Therapeutic Potential of Irrigants in Endodontic Therapy

Nogo-Živanović

Bjelović

Ivanović

et al. 2018

Serbian Journal of Experimental and Clinical Research

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Effect of the Combination of Sodium Hypochlorite and Chlorhexidine on Dentinal Permeability and Scanning Electron Microscopy Precipitate Observation

Cited by 80 publications

References 26 publications

Effect of Intermediate Flush Using Different Devices to Prevent Chemical Smear Layer Formation

Effect of Intermediate Flush Using Different Devices to Prevent Chemical Smear Layer Formation

Bioactive Chitosan Nanoparticles and Photodynamic Therapy Inhibit Collagen Degradation In Vitro

Consideration of the Therapeutic Potential of Irrigants in Endodontic Therapy

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