Effect of the Citrus Lycopene β-Cyclase Transgene on Carotenoid Metabolism in Transgenic Tomato Fruits

Guo, Fei; Zhou, Wei; Zhang, Jiancheng; Xu, Qiang; Deng, Xiuxin

doi:10.1371/journal.pone.0032221

Cited by 35 publications

(20 citation statements)

References 43 publications

(47 reference statements)

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“…5b and 6b). This finding suggested that the carotenoid levels in photosynthetic organs tend to be in equilibrium under forced gene expression conditions, being consistent in the leaf of the citrus lycopene β-cyclase transgenic tomato [24]. The fact that the expressing carotenoid genes using constitutive promoter like 35S has been hard to increase the level of carotenoid in leaf has been also presented in previous study [25].…”

Section: Discussionsupporting

confidence: 75%

Genetic Modification of the Soybean to Enhance the β-Carotene Content through Seed-Specific Expression

Kim

et al. 2012

PLoS ONE

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The carotenoid biosynthetic pathway was genetically manipulated using the recombinant PAC (Phytoene synthase-2A-Carotene desaturase) gene in Korean soybean (Glycine max L. cv. Kwangan). The PAC gene was linked to either the β-conglycinin (β) or CaMV-35S (35S) promoter to generate β-PAC and 35S-PAC constructs, respectively. A total of 37 transgenic lines (19 for β-PAC and 18 for 35S-PAC) were obtained through Agrobacterium-mediated transformation using the modified half-seed method. The multi-copy insertion of the transgene was determined by genomic Southern blot analysis. Four lines for β-PAC were selected by visual inspection to confirm an orange endosperm, which was not found in the seeds of the 35S-PAC lines. The strong expression of PAC gene was detected in the seeds of the β-PAC lines and in the leaves of the 35S-PAC lines by RT-PCR and qRT-PCR analyses, suggesting that these two different promoters function distinctively. HPLC analysis of the seeds and leaves of the T2 generation plants revealed that the best line among the β-PAC transgenic seeds accumulated 146 µg/g of total carotenoids (approximately 62-fold higher than non-transgenic seeds), of which 112 µg/g (77%) was β-carotene. In contrast, the level and composition of the leaf carotenoids showed little difference between transgenic and non-transgenic soybean plants. We have therefore demonstrated the production of a high β-carotene soybean through the seed-specific overexpression of two carotenoid biosynthetic genes, Capsicum phytoene synthase and Pantoea carotene desaturase. This nutritional enhancement of soybean seeds through the elevation of the provitamin A content to produce biofortified food may have practical health benefits in the future in both humans and livestock.

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Section: Discussionsupporting

confidence: 75%

Genetic Modification of the Soybean to Enhance the β-Carotene Content through Seed-Specific Expression

Kim

et al. 2012

PLoS ONE

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“…• Rice that produces β-carotene (provitamin A) in the endosperm (Golden Rice) (Ye et al 2000;Beyer 2010), and has increased amounts of folate, and mineral (iron and zinc) in the seed (Beyer 2010;Lee et al 2009Lee et al , 2012Yang et al 2013); • Wheat grain with enhanced levels of iron and zinc (Borg et al 2012;Sui et al 2012;Borrill et al 2014); • Potatoes that are protein-rich (Chakraborthy et al 2010), have better aroma and less browning (Llorente et al 2010), and exhibit reduced cold-induced sweetening and increased carotenoid content (Giuliano et al 2006;Chen et al 2008;Bhaskar et al 2010;Barrell et al 2013); • Tomatoes with increased lycopene and β-carotene (Guo et al 2012;Liu et al 2014);…”

Section: Biofortificationmentioning

confidence: 99%

Next Generation Plant Biotechnology

Ahuja¹

2014

Sustainable Development and Biodiversity

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Modern plant biotechnology began with the transfer of foreign chimeric genes into plants. Initially recombinant genes were derived from bacteria, animals and plants for gene transfer. Gene transfer was accomplished by Agrobacteriummediated or biolistic methods into the plant genome. The first wave of transgenic plants that were monitored for transgene integration and expression, the second wave transgenic plants carried economically important genes for herbicide tolerance, pest resistance, drought and salt tolerance, growth traits, and flowering control. Subsequently, a number of genetically modified crops with several useful traits have been commercialized. Although relatively stable transgene expression has been observed in a number of plant species, there were also unintended unstable events in transgenic plants. This is due to the fact that transgene integration achieved by the two traditional methods ( Agrobacterium or biolistic) of gene transfer in the plant genome is random, and one to several copies of the transgenes may be integrated at one or several locations in the genome. In order to overcome the problem of randomness of transgene integration, site-specific transgene integration strategies have been experimentally tested in plants, and offer prospects of stable gene integration and expression in transgenic plants. In order to broaden the scope of transgenic plants, biotechnologists started looking for other useful avenues for their utility. With finite reserves of fossil fuels and climate change, and growing demands for fuels, plastics, and pharmaceuticals, transgenic plants have been also explored as production platforms for these commodities. This paper is an overview of next generation transgenic plants that can serve as bioreactors or biofactories for the cost-effective production of biofuels, biopharmaceuticals, bioplastics, and as a resource for nutritional supplements to meet human demands in the future. New developments in nanobiotechnology offer prospects for improved production of crop plants.

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“…In this way, thanks to the new developments it has been posible to identify quantitative trait loci (QTL) as well as genes and allelic variants of these genes involved in the synthesis of compounds responsible for bioactive properties as well as molecular The improvement in the content of bioactive compounds can also be achieved by means of genetic transformation, which allows important increases in a short period of time (Díaz de la Garza et al, 2007; Guo et al, 2012). Genetic transformation requires the introduction using different transformation techniques of one or several genes from different organisms in the genome of the target species in order to achieve transgenesis (Kole et al, 2010).…”

Section: Breeding Strategiesmentioning

confidence: 99%

Breeding Vegetables with Improved Bioactive Properties

Plazas¹,

Vilanova²,

Andújar³

et al. 2014

BUASVMCN-HORT

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Vegetable crops contain signi icant amounts of many bioactive compounds which prevent and/or protect against chronic diseases. Consumers increasingly demand vegetables with improved bioactive properties and this is stimulating the development of new cultivars with enhanced content in bioactive compounds. Generally, breeding programmes of speci ic crops are aimed at increasing the most relevant bioactive compounds of each crop. The success of these breeding programmes depends on the availability of sources of variation for bioactive compounds. Traditional varieties and wild relatives collections are generally very variable for these compounds and in many cases it is possible to identify sources of variation of great interest among these materials. There are several breeding strategies for improving the content in bioactive compounds, including conventional strategies based on phenotyping, as well as modern strategies that rely on marker assisted selection or genetic transformation. Breeding for the enhancement of bioactive compounds may affect vegetables in a positive (e.g., extended shelflife) or negative (e.g., browning, bitterness) way other relevant traits for the success of a cultivar. The negative side effects may be circumvented by using complementary breeding strategies aimed at reducing or removing the negative impact on the characteristics and performance of a new cultivar. In summary, breeding can contribute to the development of a new generation of vegetable crops with enhanced bioactive properties and therefore to the development of the horticultural sector.

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Effect of the Citrus Lycopene β-Cyclase Transgene on Carotenoid Metabolism in Transgenic Tomato Fruits

Cited by 35 publications

References 43 publications

Genetic Modification of the Soybean to Enhance the β-Carotene Content through Seed-Specific Expression

Genetic Modification of the Soybean to Enhance the β-Carotene Content through Seed-Specific Expression

Next Generation Plant Biotechnology

Breeding Vegetables with Improved Bioactive Properties

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