Effect of the anti-inflammatory drug diclofenac on lipid composition of bacterial strain Raoultella sp. KDF8

Palyzová, Andrea; Marešová, Helena; Novák, Jiří; Zahradník, Jiří; Řezanka, Tomáš

doi:10.1007/s12223-020-00790-9

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…At the same time, the majority of ecotoxicological studies are devoted only to IBP itself, while information on detection and toxicity of its partial oxidation products is quite scarce [51][52][53]. There are only a few data indicating negative effects of IBP and its metabolites, other NSAIDs included, on nitrogen fixation, induction of oxidative stress in microorganisms, and destabilization of cell membranes [21,54,55]. IBP has recently been shown to facilitate the spreading of antibiotic resistance through the uptake of exogenous antibiotic resistance genes [56].…”

Section: Introductionmentioning

confidence: 99%

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

et al. 2021

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The article expands our knowledge on the variety of biodegraders of ibuprofen, one of the most frequently detected non-steroidal anti-inflammatory drugs in the environment. We studied the dynamics of ibuprofen decomposition and its relationship with the physiological status of bacteria and with additional carbon and energy sources. The involvement of cytoplasmic enzymes in ibuprofen biodegradation was confirmed. Within the tested actinobacteria, Rhodococcus cerastii IEGM 1278 was capable of complete oxidation of 100 μg/L and 100 mg/L of ibuprofen in 30 h and 144 h, respectively, in the presence of an alternative carbon source (n-hexadecane). Besides, the presence of ibuprofen induced a transition of rhodococci from single- to multicellular lifeforms, a shift to more negative zeta potential values, and a decrease in the membrane permeability. The initial steps of ibuprofen biotransformation by R. cerastii IEGM 1278 involved the formation of hydroxylated and decarboxylated derivatives with higher phytotoxicity than the parent compound (ibuprofen). The data obtained indicate potential threats of this pharmaceutical pollutant and its metabolites to biota and natural ecosystems.

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Section: Introductionmentioning

confidence: 99%

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

et al. 2021

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“…Four genes were present in the bacterial species encoding the catechol and protocatechuate branches of beta-ketoadipate metabolism, the salicylate and gentisate metabolism, as well as the homogenization pathway for the breakdown of aromatic compounds. In this case, the drug molecule was the substrate of the efflux pump involved in the resistance against the given antibiotic or a gene encoding the breakdown of non-specific aromatic intermediates, which contributes to the resistance of the microorganisms to drug molecules as the aromatic ring is present in them [ 38 , 39 , 40 , 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Anti-Inflammatory Drugs on the Prokaryotic Community Composition and Selected Bacterial Strains Based on Microcosm Experiments

Farkas,

Mireisz,

Toumi

et al. 2023

Microorganisms

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Non-steroidal anti-inflammatory drugs (NSAIDs) are increasingly recognized as potential environmental contaminants that may induce toxicity in aquatic ecosystems. This 3-week microcosm experiment explores the acute impacts of NSAIDs, including diclofenac (DCF), ibuprofen (IBU), and acetylsalicylic acid (ASA), on bacterial communities using a wide range of these substances (200–6000 ppm). The results showed that the NSAID-treated microcosms had higher cell count values than control samples, though the diversity of microbial communities decreased. The isolated heterotrophic bacteria mostly belonged to Proteobacteria, particularly Klebsiella. Next-generation sequencing (NGS) revealed that NSAIDs altered the structure of the bacterial community composition, with the proportion of Proteobacteria aligning with the selective cultivation results. Bacteria had higher resistance to IBU/ASA than to DCF. In DCF-treated microcosms, there has been a high reduction of the number of Bacteroidetes, whereas in the microcosms treated with IBU/ASA, they have remained abundant. The numbers of Patescibacteria and Actinobacteria have decreased across all NSAID-treated microcosms. Verrucomicrobia and Planctomycetes have tolerated all NSAIDs, even DCF. Cyanobacteria have also demonstrated tolerance to IBU/ASA treatment in the microcosms. The archaeal community structure was also impacted by the NSAID treatments, with Thaumarchaeota abundant in all microcosms, especially DCF-treated microcosms, while Nanoarchaeota is more typical of IBU/ASA-treated microcosms with lower NSAID concentrations. These results indicate that the presence of NSAIDs in aquatic environments could lead to changes in the composition of microbial communities.

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“…The presence of naproxen at a concentration as high as 4.8–5.2 g L −1 causes severe alterations in the membrane composition, i.e., the presence of the 16:0 iso 3OH acid only in the samples cultivated with naproxen, the slight decrease in the content of 18:0 anteiso acid at the highest tested concentration and remarkable decrease of the 18:1 ω9c acid. Palyzová et al [ 55 ] described the alterations of lipid profile after DCF exposure in the time ranging from 24 to 72 h at a concentration of 1.0 g L −1 observed for Raoultella sp. KDF8 strain.…”

Section: Discussionmentioning

confidence: 99%

“…Authors hypothesized that due to those changes, strain is able to deal with destabilization of the inner and outer membrane. Moreover, decreased amounts of phosphatidylethanolamine with prolonged incubation time, and increased amounts of phospholipids having three or four acyls i.e., acyl-phosphatidylglycerol and cardiolipin with the prolonged time of cultivation, were observed [ 15 , 51 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Diclofenac Degradation—Enzymes, Genetic Background and Cellular Alterations Triggered in Diclofenac-Metabolizing Strain Pseudomonas moorei KB4

Żur

Piński

Wojcieszyńska

et al. 2020

IJMS

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Diclofenac (DCF) constitutes one of the most significant ecopollutants detected in various environmental matrices. Biological clean-up technologies that rely on xenobiotics-degrading microorganisms are considered as a valuable alternative for chemical oxidation methods. Up to now, the knowledge about DCF multi-level influence on bacterial cells is fragmentary. In this study, we evaluate the degradation potential and impact of DCF on Pseudomonas moorei KB4 strain. In mono-substrate culture KB4 metabolized 0.5 mg L−1 of DCF, but supplementation with glucose (Glc) and sodium acetate (SA) increased degraded doses up to 1 mg L−1 within 12 days. For all established conditions, 4′-OH-DCF and DCF-lactam were identified. Gene expression analysis revealed the up-regulation of selected genes encoding biotransformation enzymes in the presence of DCF, in both mono-substrate and co-metabolic conditions. The multifactorial analysis of KB4 cell exposure to DCF showed a decrease in the zeta-potential with a simultaneous increase in the cell wall hydrophobicity. Magnified membrane permeability was coupled with the significant increase in the branched (19:0 anteiso) and cyclopropane (17:0 cyclo) fatty acid accompanied with reduced amounts of unsaturated ones. DCF injures the cells which is expressed by raised activities of acid and alkaline phosphatases as well as formation of lipids peroxidation products (LPX). The elevated activity of superoxide dismutase (SOD) and catalase (CAT) testified that DCF induced oxidative stress.

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Effect of the anti-inflammatory drug diclofenac on lipid composition of bacterial strain Raoultella sp. KDF8

Cited by 8 publications

References 40 publications

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

The Impact of Anti-Inflammatory Drugs on the Prokaryotic Community Composition and Selected Bacterial Strains Based on Microcosm Experiments

Diclofenac Degradation—Enzymes, Genetic Background and Cellular Alterations Triggered in Diclofenac-Metabolizing Strain Pseudomonas moorei KB4

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