Effect of thawing procedure on cryosurvival of deer spermatozoa: work in progress

Soler, Ana Josefa; Astore, Vanesa; Sestelo, A.; Rivolta, Miguel; Jácome, Luis Norberto; Garde, José Julián

doi:10.1016/s0093-691x(03)00043-8

Cited by 18 publications

(20 citation statements)

References 25 publications

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“…Samples were processed as soon as they arrived at the laboratory. Spermatozoa were immediately collected from one epididymis of each pair of testicles according to the method described by Soler et al. (2003).…”

Section: Methodsmentioning

confidence: 99%

Refrigerated Storage of Red Deer Epididymal Spermatozoa in the Epididymis, Diluted and with Vitamin C Supplementation

Fernández-Santos

Domínguez-Rebolledo

Esteso

et al. 2009

Reprod Domestic Animals

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We have approached the problem of refrigerated storage of epididymal sperm samples from red deer by comparing three options: storing the genital (testicles within the scrotum), diluting the semen in extender or diluting the semen in extender supplemented with an anti-oxidant. Twenty-nine pairs of testes were collected. Spermatozoa from one of each of the pairs were immediately recovered, and diluted to 400 x 10(6) sperm/ml in Tris-citrate-fructose with 20% egg yolk. Control group was stored as such, and Anti-oxidant group was supplemented with 0.8 mm vitamin C. The remaining epididymides and the diluted samples were stored at 5 degrees C and spermatozoa were analysed at 0, 24, 96 and 192 h for: motility [computer-assisted semen analysis (CASA)], acrosomal integrity, sperm viability (eosine/nigrosine staining), normal tails and chromatin status [sperm chromatin structure assay (SCSA)]. In general, seminal quality decreased with storage time. Vitamin C supported progressive motility better at 24 h (median 42% vs 23% Control and 15% epididymis), reduced the incidence of tail abnormalities and protected chromatin. Storing the semen in the epididymis slowed down motility loss, but slightly increased the occurrence of tail abnormalities and viability was lower at 192 h. However, regarding chromatin status, sperm stored in the epididymis was protected similarly to those diluted in the medium supplemented with vitamin C. Although the differences between the three groups were small, there were some advantages in supplementing the extender with vitamin C. Besides, refrigerating the epididymis may be a good option when immediate processing is not available.

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Section: Methodsmentioning

confidence: 99%

Refrigerated Storage of Red Deer Epididymal Spermatozoa in the Epididymis, Diluted and with Vitamin C Supplementation

Fernández-Santos

Domínguez-Rebolledo

Esteso

et al. 2009

Reprod Domestic Animals

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“…In another series of studies, we have dealt with the thawing procedures of epididymal sperm cryopreservation. Thus, Soler et al. (2003a,b) studied the effect of different thawing rates after freezing semen straws using liquid nitrogen vapours.…”

Section: Cryopreservation Of Red Deer Semenmentioning

confidence: 99%

The Application of Reproductive Technologies to Natural Populations of Red Deer

Garde

Martínez‐Pastor

Gomendio

et al. 2006

Reprod Domestic Animals

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Over the past decade, there has been increasing interest in the application of reproductive technology to the conservation and management of natural populations of deer. The application of assisted reproduction technologies within natural population of deer is in its infancy. However, its future potential is enormous, particularly in relation to genetic management or conservation. This paper reviews the present state of such technologies for a wild subspecies of red deer, the Iberian red deer (Cervus elaphus hispanicus), by discussing the major components of oestrous synchronization, semen collection/cryopreservation and insemination techniques. In addition, findings made during the course of studies on natural populations have enormous potential for the understanding of novel reproductive mechanism that may not be uncovered by livestock or human studies. A summary of these results are also reviewed here.

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“…The resistance of spermatozoa to the thawing process is dependent on the basic extender used and the concentration of cryoprotectant as they interact with the freezing and thawing rates (Curry and Watson, 1994; Curry 2000). This sensitivity of the spermatozoa to thawing temperatures appears to differ between species (Fiser et al, 1981; Arav et al, 1994; Söderquist et al, 1997; Younis et al, 1998; Eriksson et al, 2000; Sukhato et al, 2001; Soler et al, 2003b) and between reproductive tract sources as has been shown in African buffalo sperm samples (Lambrechts et al, 1999). The effect of reproductive tract source (ejaculated vs epididymal) on sperm resistance to the thawing process might be related to different environment conditions between epididymal and ejaculated spermatozoa.…”

mentioning

confidence: 98%

Effects of Thawing Procedure on Postthawed In Vitro Viability and In Vivo Fertility of Red Deer Epididymal Spermatozoa Cryopreserved at −196°C

Soler¹,

García

Fernández-Santos³

et al. 2003

Journal of Andrology

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ABSTRACT:In this study, we have determined the effects of individual factor and thawing procedure on in vitro viability and in vivo fertility of frozen-thawed red deer epididymal spermatozoa. The spermatozoa that were collected from 13 Iberian deer stags were diluted at room temperature in a Triladylா-20% egg yolk medium and frozen in nitrogen vapors. In the first experimental series, sperm samples were collected from 10 mature stags. For thawing, the frozen straws were subjected to 3 different procedures: I (37ЊC for 20 seconds), II (60ЊC for 8 seconds) and III (70ЊC for 5 seconds). Sperm cryosurvival was judged in vitro by microscopic assessments of individual sperm motility (SM) and of plasma membrane and acrosome (NAR) integrities. Statistically significant variations were found (P Ͻ .05) between stags for most of the seminal parameters evaluated. The thawing procedure did not have an effect on the seminal characteristics evaluated after this process, except for SM (P Ͻ .05), with the best overall recovery rates after freezing and thawing found with the use of protocol I. Our results also show a differential resistance to return to isosmotic conditions of spermatozoa thawed using the different thawing protocols. In the second experimental series (insemination artificial trial), with spermatozoa from 3 stags, results of fertility were statistically higher (69.7% vs 42.4%, P ϭ .014) when spermatozoa were thawed at 37ЊC for 20 seconds than were warmed at 60ЊC for 8 seconds. Therefore, thawing protocol I, which provides slow thawing rates, was the most beneficial for epididymal spermatozoa thawing of the cervid subspecies analyzed in this study. In summary, high in vitro survival and in vivo fertility of frozenthawed deer epididymal spermatozoa were dependent on warming rates, but each stag exhibited its own sensitivity to cryopreservation.

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Effect of thawing procedure on cryosurvival of deer spermatozoa: work in progress

Cited by 18 publications

References 25 publications

Refrigerated Storage of Red Deer Epididymal Spermatozoa in the Epididymis, Diluted and with Vitamin C Supplementation

Refrigerated Storage of Red Deer Epididymal Spermatozoa in the Epididymis, Diluted and with Vitamin C Supplementation

The Application of Reproductive Technologies to Natural Populations of Red Deer

Effects of Thawing Procedure on Postthawed In Vitro Viability and In Vivo Fertility of Red Deer Epididymal Spermatozoa Cryopreserved at −196°C

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