Refrigerated Storage of Red Deer Epididymal Spermatozoa in the Epididymis, Diluted and with Vitamin C Supplementation

Fernández-Santos, M.R.; Domínguez-Rebolledo, Álvaro E.; Esteso, M.C.; Garde, José Julián; Martínez‐Pastor, Felipe

doi:10.1111/j.1439-0531.2007.01032.x

Cited by 34 publications

(19 citation statements)

References 56 publications

(100 reference statements)

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“…Correlation between RFM and storage time was -0.54±0.18. Similar data were obtained in studies of low-temperature postmortal storage of epididymides of mice [12], sheep [11], deer [8,9], dogs [15], and feline species [6,10]. These studies revealed a negative effect of postmortal storage of the epididymides on RFM of native and frozen-thawed spermatozoa.…”

Section: Effects Of Cooling and Storage Time Of The Mouse Carcass At supporting

confidence: 82%

Viability of Postmortal Epididymal Mouse Spermatozoa during Long-Term Hypothermic Storage and Cryopreservation

2011

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The study examined the effect of long-term hypothermic (4°C) storage of mouse carcasses on motility, cell membrane damage, in vitro survival and capacitation of epididymal spermatozoa before and after cryopreservation. It was shown that the number of spermatozoa with rectilinear forward motion decreased with increasing storage time. There were no significant changes in the total sperm motility and integrity of their plasmalemma. Pronounced effects of hypothermia and long-term storage of the mouse carcasses on cryocapacitation of spermatozoa during cryoconservation were demonstrated.

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Section: Effects Of Cooling and Storage Time Of The Mouse Carcass At supporting

confidence: 82%

Viability of Postmortal Epididymal Mouse Spermatozoa during Long-Term Hypothermic Storage and Cryopreservation

2011

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“…In addition, cold storage for 24 hr did not affect the Siamese Eld's deer epididymal sperm motility and membrane and DNA integrity in the present study. This finding was in accordance with the studies in sika and red deer [3,4] and domestic cats [2]. During 24-hr of cold storage, anti-oxidative mechanism in the sperm cells might neutralize lipid peroxidation, a cause of sperm damage, as was shown in the horse [7].…”

supporting

confidence: 91%

A Case Report Concerning Male Gametes Rescued from a Siamese Eld’s Deer (<i>Rucervus eldii siamensis</i>): Post-Thawed Testicular and Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability

Thuwanut

Thongphakdee

Sommanustweechai

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. In the present study, the quality of frozen-thawed epididymal and testicular sperm recovered from a Siamese Eld's deer was examined. The epididymal sperm quality was assessed in fresh, cold-stored at 4°C and frozen-thawed samples. Zona binding ability of the frozen-thawed epididymal samples with Burmese Eld's deer oocytes was also evaluated. Testicular sperm extracted from tissues frozen at −80 or −196°C for one month were examined for membrane and DNA integrity. Epididymal sperm retained their quality for up to 24 hr of cold storage at 4°C. The percentages of sperm motility, intact membrane, intact acrosome and intact DNA were 30, 46.5, 27 and 89.5% in the frozen and thawed epididymal sperm, and the average ability to bind with oocytes was 92.5 ± 64 sperm/oocytes. Around 70% of the sperm extracted from testicular tissues cryopreserved at −196 and −80°C for one month showed an intact membrane. In conclusion, epididymal and testicular sperm survived for more than 13 hr post-mortem. Furthermore, cold storage at 4°C and cryopreservation at −196 and −80°C maintain the quality of epididymal and testicular sperm. This study represents a model for male gamete rescue in endangered Eld's deer. KEY WORDS: Siamese Eld's deer, sperm collection post mortem, sperm cryopreservation.doi: 10.1292/jvms.11-0491; J. Vet. Med. Sci. 75(1): 123-125, 2013 Cryotechnology of genetic resources is notable as a potential tool to conserve germplasm of endangered wild species [5]. While ejaculated sperm is recovered from live animals, epididymides and testes are potential major sources of male gametes in animals that die unexpectedly [8]. Live births have been produced using cryopreserved epididymal sperm in many species [10,15]. In addition, intracytoplasmic sperm injection (ICSI) with testicular sperm was successful in the human [6]. Siamese Eld's deer (Rucervus eldii siamensis) are native to Thailand but have been extirpated in the wild and are found only in captivity [12]. Although production of offspring after intrauterine insemination with frozen-thawed ejaculated Burmese Eld's deer sperm was reported [9], there are no studies of Siamese Eld's deer reproductive biology. The aim of this study was to evaluate and cryopreserve male gametes recovered from the epididymis and testes of a Siamese Eld's deer that died in captivity.A two-year-old male Siamese Eld's deer maintained at Dusit Zoo, Bangkok, Thailand, unexpectedly died of an unknown cause at 11:00 pm on January 18, 2009, and was kept in a refrigerator until the next day (10:00 am). The right testis with epididymis was removed, stored in phosphate buffered saline at 4°C and transported to a laboratory within 2 hr. Epididymal sperm was collected by placing the caudal epididymis into 10 ml pre-warmed phosphate buffer saline (PBS), cut into small pieces and incubated at 37°C for 10 min. After epididymal tissues were removed, sperm samples were evaluated for sperm concentration, total number, motility (20× magnification with a phase contrast microscope), morphology (William's...

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“…One of the most commonly reported is sperm processing, especially centrifugation and incubation (Bungum et al 2008;Koderle et al 2009;Matsuura et al 2010). Another potential factor is the use of different types of extenders (Shahiduzzaman and Linde-Forsberg 2007;Fernandez-Santos et al 2009;Morrell et al 2009). The purpose of short-term preservation of dog sperm is to maintain fertilization potential for a longer time than is the case for fresh semen.…”

mentioning

confidence: 99%

DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa

Prinosilova¹,

Rybar²,

Zajicova³

et al. 2012

Vet. Med. - Czech

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Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by the sperm chromatin structure assay (SCSA). In the first experiment, the chromatin integrity of fresh semen from 60 dogs with different historiy of fertility was compared with other sperm parameters (total sperm count, sperm motility, viability, acrosomal integrity and sperm morphology). Except for 15 dogs that had never mated before, all were used in breeding as semen donors. Thirty-three of them were successful breeding males while in 12 repeated fertility problems were noted. Ejaculates were assigned to groups with good and poor quality, based on determined sperm motility and percentage of morphologically normal sperm. In the second experiment, chromatin status was measured in fresh and chilled spermatozoa (on day 0, 2, 4, 6, 8 and 10 of storage). Finally, in the third experiment, the chromatin status was measured in fresh and cryopreserved spermatozoa. Evaluating fresh dog semen, we observed that DNA fragmentation index (DFI) and percentage of cells with high DNA stainability (HDS) negatively correlated with total sperm count, percentage of total and progressively motile spermatozoa, sperm viability and percentage of morphologically normal spermatozoa, even with rather low correlation indices. Lower chromatin integrity was found in the group of dog ejaculates showing poor quality in comparison with the group of good quality ejaculates. All dogs with repeated fertility problems were classed in the group showing poor quality, and even though their DFI was significantly higher than the DFI of successful breeding males, the highest DFI we obtained was only just below 9%. We can assume that the chromatin damage level in any of the evaluated dogs was not high enough to have a significant effect on their fertility. Concerning the potential cause of reduced male fertility, the assessment of chromatin integrity in fresh dog ejaculates failed to add any additional information to the results obtained by other techniques of semen analysis. Thus, the current study indicates that neither 10-day preservation of canine sperm chilled in commercial extenders nor long-term cryopreservation in extenders recommended for canine sperm preservation produce adverse effects on sperm chromatin integrity.

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Refrigerated Storage of Red Deer Epididymal Spermatozoa in the Epididymis, Diluted and with Vitamin C Supplementation

Cited by 34 publications

References 56 publications

Viability of Postmortal Epididymal Mouse Spermatozoa during Long-Term Hypothermic Storage and Cryopreservation

Viability of Postmortal Epididymal Mouse Spermatozoa during Long-Term Hypothermic Storage and Cryopreservation

A Case Report Concerning Male Gametes Rescued from a Siamese Eld’s Deer (<i>Rucervus eldii siamensis</i>): Post-Thawed Testicular and Epididymal Sperm Quality and Heterologous Zona Pellucida Binding Ability

DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa

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