Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes

Nicolau-Solano, S. I.; Doran, Olena

doi:10.1016/j.livsci.2007.05.001

Cited by 8 publications

(3 citation statements)

References 34 publications

(42 reference statements)

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“…High 3␤-HSD expression and activity enhance androstenone metabolism and thereby decrease the accumulation of androstenone in adipose tissue. Nicolau-Solano and Doran [34] showed that in vitro 3␤-HSD protein expression in primary porcine hepatocytes increased upon treatment with androstenone and estrone sulfate. This effect was observed in hepatocytes isolated from heavier pigs (90 kg) and only in a limited range of androstenone and estrone sulfate concentrations.…”

Section: Discussionmentioning

confidence: 99%

Feeding dried chicory root to pigs decrease androstenone accumulation in fat by increasing hepatic 3β hydroxysteroid dehydrogenase expression

Rasmussen

Brunius

Zamaratskaia

et al. 2012

The Journal of Steroid Biochemistry and Molecular Biology

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Section: Discussionmentioning

confidence: 99%

Feeding dried chicory root to pigs decrease androstenone accumulation in fat by increasing hepatic 3β hydroxysteroid dehydrogenase expression

Rasmussen

Brunius

Zamaratskaia

et al. 2012

The Journal of Steroid Biochemistry and Molecular Biology

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“…In a study using porcine hepatocytes isolated from intact male pigs weighing 70 and 92 kg, different response patterns to steroid treatment depending on weight were discovered. In low-weight pigs, estrone sulfate produced no effect on 3β-HSD protein expression, whereas a significant induction was present in the hepatocytes from heavy-weight pigs [52]. Similarly, androstenone produced an increase in protein expression in heavy-weight pigs, whereas it had no effect on low-weight pigs.…”

Section: Transcriptional Regulation Of 3β-hsdmentioning

confidence: 99%

Regulation of 3β-Hydroxysteroid Dehydrogenase/Δ5-Δ4 Isomerase: A Review

Rasmussen

Ekstrand

Zamaratskaia

2013

IJMS

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This review focuses on the expression and regulation of 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (3β-HSD), with emphasis on the porcine version. 3β-HSD is often associated with steroidogenesis, but its function in the metabolism of both steroids and xenobiotics is more obscure. Based on currently available literature covering humans, rodents and pigs, this review provides an overview of the present knowledge concerning the regulatory mechanisms for 3β-HSD at all omic levels. The HSD isoenzymes are essential in steroid hormone metabolism, both in the synthesis and degradation of steroids. They display tissue-specific expression and factors influencing their activity, which therefore indicates their tissue-specific responses. 3β-HSD is involved in the synthesis of a number of natural steroid hormones, including progesterone and testosterone, and the hepatic degradation of the pheromone androstenone. In general, a number of signaling and regulatory pathways have been demonstrated to influence 3β-HSD transcription and activity, e.g., JAK-STAT, LH/hCG, ERα, AR, SF-1 and PPARα. The expression and enzymic activity of 3β-HSD are also influenced by external factors, such as dietary composition. Much of the research conducted on porcine 3β-HSD is motivated by its importance for the occurrence of the boar taint phenomenon that results from high concentrations of steroids such as androstenone. This topic is also examined in this review.

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“…Glucocorticoids are known to stimulate 3b-HSD expression in human adrenocortical NCI-H295R cells (Feltus et al 2002), but reduce 3b-HSD expression and activity in rat Leydig cells (Xiao et al 2010). Androgens are reported to inhibit 3b-HSD expression in Leydig cells (Stalvey & Clavey 1992;Heggland et al 1997) and adrenal fasciculata cells (Perry & Stalvey 1992), but enhance 3b-HSD protein expression in primary cultured pig hepatocytes in a concentration-dependent manner (Nicolau-Solano & Doran 2008). However, the majority of studies have been carried out on the 3b-HSD gene regulation in steroidogenic tissues in humans or rodents; little is known about the transcriptional regulation of the 3b-HSD gene in porcine liver.…”

Section: Introductionmentioning

confidence: 99%

Glucocorticoid receptor is involved in the differential expression of hepatic 3β‐hydroxysteroid dehydrogenase between barrows and boars at finishing stage

Cong

Yao

et al. 2017

Animal Science Journal

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The enzyme 3β-hydroxysteroid dehydrogenase (3β-HSD) plays an important role in androstenone metabolism in pig liver, and its defective expression is related to the development of boar taint. Early age castration is a common practice in many countries to avoid boar taint, yet whether and how castration affects porcine hepatic 3β-HSD expression are still poorly understood. In this study, we aimed to compare the expression of 3β-HSD between intact (boars) and castrated (barrows) male pigs, and to explore the potential factors regulating 3β-HSD transcription. Compared to barrows, boars showed worse carcass quality. Boars had significantly higher levels of serum androstenone (P < 0.01), testosterone (P < 0.01) and hepatic cortisol (P < 0.05), which were contrary to significantly lower expression of 3β-HSD messenger RNA (P < 0.01) and protein (P < 0.01) in the liver. Significant differences were detected for the hepatic expression of androgen receptor (AR) and CCAAT/enhancer binding protein β (C/EBPβ). Chromatin immunoprecipitation (ChIP) assay demonstrated reduced histone H3 acetylation (P < 0.05) but increased glucocorticoid receptor (GR) binding to 3β-HSD gene promoter in boars (P < 0.05). These results indicate that GR binding to 3β-HSD promoter is involved in the differential hepatic 3β-HSD expression between boars and barrows.

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Effect of testosterone, estrone sulphate and androstenone on 3β-hydroxysteroid dehydrogenase protein expression in primary cultured hepatocytes

Cited by 8 publications

References 34 publications

Feeding dried chicory root to pigs decrease androstenone accumulation in fat by increasing hepatic 3β hydroxysteroid dehydrogenase expression

Feeding dried chicory root to pigs decrease androstenone accumulation in fat by increasing hepatic 3β hydroxysteroid dehydrogenase expression

Regulation of 3β-Hydroxysteroid Dehydrogenase/Δ5-Δ4 Isomerase: A Review

Glucocorticoid receptor is involved in the differential expression of hepatic 3β‐hydroxysteroid dehydrogenase between barrows and boars at finishing stage

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