Effect of temporally controlled release of dexamethasone on in vivo chondrogenic differentiation of mesenchymal stromal cells

Bae, Soon Eon; Choi, Dong Hoon; Han, Dong Keun

doi:10.1016/j.jconrel.2009.12.024

Cited by 31 publications

(22 citation statements)

References 27 publications

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“…154,235 The rate of release of dexamethasone was controlled from hydrogels composed of Pluronic and hyaluronic acid through the use of porous and nonporous microparticles of poly(lactic-co-glycolic acid). 236 The faster release profile, which was 100% release in 4 weeks, resulted in greater chondrogenesis by encapsulated MSCs after 4 weeks of subcutaneous implantation. 236 Heparin was bound to TGFb3 to retard its release profile, resulting in enhanced cartilage production by chondrocytes in PNIPAAm-based hydrogels compared to the faster release of TGFb3.…”

Section: Controlled Release Of Growth Factorsmentioning

confidence: 98%

“…236 The faster release profile, which was 100% release in 4 weeks, resulted in greater chondrogenesis by encapsulated MSCs after 4 weeks of subcutaneous implantation. 236 Heparin was bound to TGFb3 to retard its release profile, resulting in enhanced cartilage production by chondrocytes in PNIPAAm-based hydrogels compared to the faster release of TGFb3. 227 Although the TGFb1, -2, and -3 isoforms have different functions in embryonic chondrogenesis, 237 their effects have not been directly compared in a cartilage tissue engineering application.…”

Section: Controlled Release Of Growth Factorsmentioning

confidence: 98%

See 1 more Smart Citation

Hydrogels for the Repair of Articular Cartilage Defects

Spiller

Maher

Lowman

2011

Tissue Engineering Part B: Reviews

400

312

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Section: Controlled Release Of Growth Factorsmentioning

confidence: 98%

Section: Controlled Release Of Growth Factorsmentioning

confidence: 98%

Hydrogels for the Repair of Articular Cartilage Defects

Spiller

Maher

Lowman

2011

Tissue Engineering Part B: Reviews

400

312

View full text Add to dashboard Cite

“…44 Recent work has exploited poly(lactic-co-glycolic acid) (PLGA) systems for the delivery of anti-inflammatory drugs. [45][46][47][48][49][50][51][52][53] Higaki et al demonstrated that the continuous administration of betamethasone sodium phosphate through PLGA nanoparticles provided increased inhibition of inflammation in an experimental model of OA when compared with the same dosage of betamethasone sodium phosphate delivered three times through IA injection. 54 Dang et al demonstrated that dex releasing PLGA microparticles are capable of suppressing the host response to implanted polymer materials in a mouse model.…”

Section: Introductionmentioning

confidence: 99%

Dexamethasone Release from Within Engineered Cartilage as a Chondroprotective Strategy Against Interleukin-1α

RoachBrendan

Kelmendi-DokoArta

BalutisElaine

et al. 2016

Tissue Engineering Part A

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“…41 A similarly designed scaffold could be used to regulate stem cell differentiation in vitro or in vivo with controlled release of multiple cues. 42 …”

Section: Resultsmentioning

confidence: 99%

Bioactive Hydrogels with Enhanced Initial and Sustained Cell Interactions

et al. 2013

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The highly tunable properties of poly(ethylene glycol) (PEG)-based hydrogel systems permit their use in a wide array of regenerative medicine and drug delivery applications. One of the most valuable properties of PEG hydrogels is their intrinsic resistance to protein adsorption and cell adhesion, as it allows for a controlled introduction of desired bioactive factors including proteins, peptides, and drugs. Acrylate-PEG-N-hydroxysuccinimide (Acr-PEG-NHS) is widely utilized as a PEG linker to functionalize bioactive factors with photocrosslinkable groups. This enables their facile incorporation into PEG hydrogel networks or the use of PEGylation strategies for drug delivery. However, PEG linkers can sterically block integrin binding sites on functionalized proteins and reduce cell-material interactions. In this study we demonstrate that reducing the density of PEG linkers on protein backbones during functionalization results in significantly improved cell adhesion and spreading to bioactive hydrogels. However, this reduction in functionalization density also increases protein loss from the matrix over time due to ester hydrolysis of the Acr-PEG-NHS linkers. To address this, a novel PEG linker, acrylamide-PEG-isocyanate (Aam-PEG-I), with enhanced hydrolytic stability was synthesized. It was found that decreasing functionalization density with Aam-PEG-I resulted in comparable increases in cell adhesion and spreading to Acr-PEG-NHS systems while maintaining protein and bioactivity levels within the hydrogel network over a significantly longer time frame. Thus, Aam-PEG-I provides a new option for protein functionalization for use in a wide range of applications that improves initial and sustained cell-material interactions to enhance control of bioactivity.

show abstract

Effect of temporally controlled release of dexamethasone on in vivo chondrogenic differentiation of mesenchymal stromal cells

Cited by 31 publications

References 27 publications

Hydrogels for the Repair of Articular Cartilage Defects

Hydrogels for the Repair of Articular Cartilage Defects

Dexamethasone Release from Within Engineered Cartilage as a Chondroprotective Strategy Against Interleukin-1α

Bioactive Hydrogels with Enhanced Initial and Sustained Cell Interactions

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