Effect of temperature on the reversed micellar extraction of enzymes

Affinity extraction of proteins with a reversed micellar system composed of Cibacron Blue-modified lecithin

“…The water concentration was lower at higher temperature because the increase of temperature could dehydrate reversed micelles (Dekker et al, 1991). Lower temperature was favorable for lysozyme extraction because of higher water concentration.…”

“…The polar headgroups of the surfactants surround water pools within which hydrophilic molecules such as amino acids, peptides, and proteins can be solubilized (Luisi, 1985). Therefore reversed micelles have been extensively studied for the extraction of these biosubstances (Dekker et al, 1989(Dekker et al, , 1990(Dekker et al, , 1991Goklen and Hatton, 1987;Hu and Gulari, 1996;Ichikawa et al, 1992Ichikawa et al, , 1996Krei and Hustedt, 1992;Kuboi et al, 1990;Lye et al, 1994). Among the variety of publications, anionic surfactants such as sodium bis(2-ethylhexyl) sulfosuccinate (AOT) (Goklen and Hatton, 1987;Ichikawa et al, 1992Ichikawa et al, , 1996Lye et al, 1994) and sodium bis(2-ethylhexyl) phosphate (Hu and Gulari, 1996) or cationic surfactants such as trioctylmethylammonium chloride (Dekker et al, 1989(Dekker et al, , 1990(Dekker et al, , 1991Krei and Hustedt, 1992) and cetyltrimethylammonium bromide (Krei and Hustedt, 1992) were mostly employed to form the extractive reversed micellar systems.…”

Integrated reversed micellar extraction and stripping of ?-amylase

“…␣-Amylase of this species has a molecular weight of 50 kDa, its isoelectric point (pI) is at pH ∼ 5.2 (Krei and Hustedt, 1992). At pH > pI the net charge of the enzyme molecules is negative and therefore, the enzyme could be removed from the source aqueous solution by cationic surfactant containing organic solvent (Brandani et al, 1996;Chang and Chen, 1995;Dekker et al, 1986;-1991Hilhorst et al, 1995).…”

Effect of hexanol on the reversed micelles of Span 85 modified with Cibacron Blue F-3GA for protein solubilization

“…For example, reversed micelles can be used for liquid-liquid extraction of proteins [1][2][3], enzymatic catalysis [4,5] and protein refolding [6,7]. Because ionic surfactants can form big and stable reversed micelles in organic solvents, ionic reversed micellar systems have mostly been investigated for the solubilization of biosubstances [8][9][10][11]. In these ionic micellar systems, the electrostatic attraction between the inner micellar charge wall and the biomolecules is the main driving force for the biomolecules transfer from a bulk aqueous phase to the polar core of reversed micelles [12].…”