Effect of Temperature on Precipitation Rate of Calcium Carbonate Produced through Microbial Metabolic Process of Bio Materials

Putri, Prima Yane; Kawaai, Keiyu; Ujike, Isao; Yamamoto, Saya

doi:10.9744/ced.18.2.103-108

Cited by 7 publications

(10 citation statements)

References 9 publications

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“…Otherwise, the precipitation rate of the calcium carbonate was slower when the temperature was lower. According to Putri [30], the 20-30 • C range is the optimum temperature range for increasing the precipitation of calcium carbonate.…”

Section: Effect Of Temperaturementioning

confidence: 99%

Calcium Carbonate in Bio-Based Material and Factor Affecting Its Precipitation Rate for Repairing Concrete

et al. 2020

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The use of bio-based material for repairing concrete is a relatively new method. Therefore, more results from simulated real-condition experiments are needed before being applied on a practical scale. In the recent past, several studies have been conducted on the improvement of bio-based repair materials. In this study, the bio-based material involving yeast, glucose, and calcium acetate mixed in a Tris buffer solution showed the potential to develop a microbial process leading to the precipitation of calcium carbonate. We investigated the factors affecting the precipitation rate of the calcium carbonate of bio-based materials for repairing leakage in the concrete specimens. Based on a series of experiments involving temperature, the type of dry yeast, and the concentration of the Tris buffer solution, the composition of bio-based materials with the highest precipitation rate of calcium carbonate was selected. The selected mixture could be applied to repair leakage of concrete until the cracks are sealed entirely.

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Section: Effect Of Temperaturementioning

confidence: 99%

Calcium Carbonate in Bio-Based Material and Factor Affecting Its Precipitation Rate for Repairing Concrete

et al. 2020

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“…It has been reported that the precipitation rate was largely dependent on the concentration of constituents, pH levels, and ambient environment such as temperature. The effect of temperature is significant in facilitating the precipitation process (Putri et al, 2016), thus leading to a larger amount of precipitates formed compared to those tested in normal room conditions.…”

Section: Introductionmentioning

confidence: 99%

Effect of Crack Repair by Bio-Based Materials Using Alginate and Bacillus Subtilis under Wet and Dry Environment Part-I

Kawaai¹,

Nishida²,

Saitô³

2020

XV International Conference on Durability of Building Materials and Components. eBook of Proceedings

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This study examined crack repair methods using alginate gel films (1.5 wt.%) mixed with healing agents under wet and dry actions. The healing agents consist of Bacillus subtilis (natto) as an aerobic microorganism and glucose as an organic carbon source, thereby producing insoluble calcium carbonate in the gel films in the presence of calcium ions. In this study, repaired surface in cracked mortar specimens (50×100mm) was observed by microscope. In addition, water absorption test was carried out in assessing the effectiveness of crack repair for the cases of alginate gel films with or without calcium carbonate precipitation induced by the microbial activities. Based on the observations made by microscope, precipitates in white color were clearly observed after 8 weeks on the cracked surface of specimens especially under wet conditions. In the case of the specimens with higher sealing rate of crack repair, the alginate gel films were remained intact under dry and wet actions, which is advantageous to keep moisture by swelling. Water absorption test was carried out using repaired mortar specimens with average crack size of 0.5 mm. Based on the results obtained, water absorption rate is substantially decreased in the specimens with increasing sealing rate of crack repair. The results suggest that higher crack sealing rate associated with calcium carbonate precipitation leads to higher resistance against water absorption tested in accordance with ASTM C 1585.

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“…Putri, et al [22]- [24] observed precipitation of calcium carbonate caused by metabolic activity of stagnant bio-based material in the test tubes. The precipitates are mainly comprised of calcium carbonate up to 48h after mixed.…”

Section: Introductionmentioning

confidence: 99%

Application of bio-based material for concrete repair: case study leakage on parallel concrete slab

2019

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The applicability of bio-based materials for concrete repair has been studied. This technique employs yeast, glucose and calcium acetate mixed in Tris buffer solution. The microbial metabolic process leads to precipitation of calcium carbonate. First, this study investigated the applicability of bio-based repair materials to small-scale concrete specimens. On this research, water permeability test was carried out to evaluate the effectiveness of the selected mixtures for sealing cracks in the concrete specimens. As the result of permeability tests carried out using specimens with crack width of 0.6 mm, water leakage through crack was observed to be negligible after 216 hours by continuous pouring method using bio-based repair materials. Also, this study showed the initial flow rate for the specimens with the same crack width does not influence crack sealing time. Furthermore, the precipitation of the calcium carbonate from the bio-based materials was analyzed by Fourier-Transformed Infra-Red spectroscopy (FT-IR) and then examined by X-ray Diffraction (XRD) for mineral identification formed through the microbial metabolic process.

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Effect of Temperature on Precipitation Rate of Calcium Carbonate Produced through Microbial Metabolic Process of Bio Materials

Cited by 7 publications

References 9 publications

Calcium Carbonate in Bio-Based Material and Factor Affecting Its Precipitation Rate for Repairing Concrete

Calcium Carbonate in Bio-Based Material and Factor Affecting Its Precipitation Rate for Repairing Concrete

Effect of Crack Repair by Bio-Based Materials Using Alginate and Bacillus Subtilis under Wet and Dry Environment Part-I

Application of bio-based material for concrete repair: case study leakage on parallel concrete slab

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