Effect of taurine on oxidative stress and apoptosis-related protein expression in trinitrobenzene sulphonic acid-induced colitis

Giriş, Murat; Depboylu, Bilge; Doğru‐Abbasoğlu, Semra; Erbil, Yeşim; Olgaç, Vakur; Alış, Halil; Aykaç‐Toker, Gülçin; Uysal, Müjdat

doi:10.1111/j.1365-2249.2008.03599.x

Cited by 53 publications

(35 citation statements)

References 38 publications

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“…This concurs with the high levels of taurine, as taurine has been shown to possess antioxidant, antiapoptotic and anti-inflammatory effects. 32 Surprisingly, glutamine, glutamate, glutathione, and ascorbate were found not to be elevated in the colonocytes from patients with active UC compared to controls, and glutamine was even found with a trend toward reduced levels. Two important notions can be made from this observation: one, the increased levels of glutamine and glutathione found in the biopsies from patients with active UC are most likely due to activated lymphocytes in the lamina propria, as these metabolites were prominent features of the metabolic profile of peripheral lymphocytes ( Figure 1E); two, it raises the question of this circumstance being a part of the pathophysiology of UC, as it has been proposed that gut mucosal turnover and barrier function is compromised due, in part, to relative glutamine and glutathione deficiency.…”

Section: Discussionmentioning

confidence: 92%

Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

et al. 2009

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Nuclear magnetic resonance (NMR) spectroscopy and appropriate multivariate statistical analyses have been employed on mucosal colonic biopsies, colonocytes, lymphocytes, and urine from patients with ulcerative colitis (UC) and controls in order to explore the diagnostic possibilities, define new potential biomarkers, and generate a better understanding of the pathophysiology. Samples were collected from patients with active UC (n = 41), quiescent UC (n = 33), and from controls (n = 25) and analyzed by NMR spectroscopy. Data analysis was carried out by principal component analysis and orthogonal-projection to latent structure-discriminant analysis using the SIMCA P+11 software package (Umetrics, Umea, Sweden) and Matlab environment. Significant differences between controls and active UC were discovered in the metabolic profiles of biopsies and colonocytes. In the biopsies from patients with active UC higher levels of antioxidants and of a range of amino acids, but lower levels of lipid, glycerophosphocholine (GPC), myo-inositol, and betaine were found, whereas the colonocytes only displayed low levels of GPC, myo-inositol and choline. Interestingly, 20% of inactive UC patients had similar profiles to those who were in an active state. This study demonstrates the possibilities of metabonomics as a diagnostic tool in active and quiescent UC and provides new insight into pathophysiologic mechanisms.

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Section: Discussionmentioning

confidence: 92%

Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

et al. 2009

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“…Immune modulation was demonstrated by experimental studies that localized membrane progesterone receptors (mPRs) on CD4 and CD8 T-lymphocytes 3 . Moreover it has been experimentally confirmed that progesterone inhibits the development of oxygen-centered free radicals and prevents cell damage after development of benzene induced toxicity as shown from the levels of lipid peroxidation (malondialdehit), glutathione (GSH) and cytochrome P450 2E1 in the liver and kidney tissues 4 .…”

Section: Introductionmentioning

confidence: 99%

“…One candidate agent is progesterone which was found to depress the inflammatory response and reduce the cell membrane damage caused by lipid peroxidation and development of free radicals [2][3][4] . Immune modulation was demonstrated by experimental studies that localized membrane progesterone receptors (mPRs) on CD4 and CD8 T-lymphocytes 3 .…”

Section: Introductionmentioning

confidence: 99%

The effect of progesterone in the prevention of the chemically induced experimental colitis in rats

Karatepe¹,

Altıok²,

Battal³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To study the effects of progesterone on an experimental colitis model. METHODS:Wistar albino rats were treated subcutaneously with 2mg/kg once a day during seven days Colitis was induced by intrarectal administration of 5mg trinitrobenzene sulfonic acid (TNBS). Disease activities, macroscopic and microscopic scores were evaluated.To determine the response provoked by progesterone we measured Colonic malondialdehyde (MDA), TNF alfa, IL-6 and Nitric oxide (NO) levels in addition to the MPO (Myeloperoxidase) and caspase-3 activities. RESULTS:Progesterone ameliorated significantly the macroscopic and microscopic scores. TNBS-induced colitis significantly increased the colonic MDA levels and caspase-3 activities in group 2 in comparison to the control group. The results of the study revealed a decline in MDA, NO, IL6 and TNF-α levels in the colon tissue and in blood due to progesterone therapy in group 3 when compared to the group 2, a significant improvement. Progesterone treatment was associated with decreased MDA, MPO, TNF alfa and caspase-3 activity. CONCLUSION:Progesterone therapy decreased oxidative damage in the colonic mucosa.Key words: Progesterone. Colitis. Inflammation. Colon. Rats. RESUMO OBJETIVO:Investigar os efeitos da progesterona em um modelo de colite experimental. MÉTODOS:Ratos albinos Wistar foram tratados subcutaneamente com 2mg/kg por dia durante sete dias. A colite foi induzida por administração intrarretal de 5mg ácido sulfônico trinitrobenzeno (TNBS). Foram avaliadas as atividades da doença, escores macroscópicos e microscópicos Para determinar a resposta provocada pela progesterona foi medida no cólon os níveis de malondialdeído (MDA), TNF alfa, IL-6 e óxido nítrico (NO), além da atividade da MPO (Myeloperoxidase) e caspase-3. RESULTADOS:A progesterone melhorou significantemente os escores macroscópicos e microscópicos. A colite induzida pelo TNBS significantemente aumentou os níveis colônicos de MDA e a atividade da caspase-3 no grupo 2 em comparação com o grupo controle.Os resultados do estudo revelaram um declínio nos níveis de MDA, NO, IL6 e TNF-α no tecido colônico e no sangue devido à terapia com a progesterona no grupo 3 quando comparado ao grupo 2. O tratamento com a progesterona foi associado com decréscimo do MDA, MPO, TNF alfa e atividade da caspase-3. CONCLUSÃO:A terapia com progesterona decresce o dano oxidativo na mucosa do cólon.Descritores: Progesterona. Colite. Inflamação. Colo. Ratos.Karatepe O et al.

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“…Treatment with urokinase alone was effective in reducing infarct volume at 4, 6 h but not 8 h after ischemia, incidence of hemorrhagic transformation were increased along with delayed thrombolysis treatment. When perfusion was recovered after administration of urokinase, it made taurine more easily access to ischemic tissue, the entering taurine could attenuate inflammatory reaction, oxidative stress, disruption of brain blood barrier and apoptosis [14,[30][31][32][33][34], finally extended the therapeutic window until to 8 h after the onset, which would lead to an increased number of patients being eligible for thrombolytic therapy.…”

Section: Discussionmentioning

confidence: 99%

A Combined Treatment with Taurine and Intra-arterial Thrombolysis in an Embolic Model of Stroke in Rats: Increased Neuroprotective Efficacy and Extended Therapeutic Time Window

Guan

Zhao

2010

Transl. Stroke Res.

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Combination treatment may target different pathophysiological events following cerebral ischemia thus enhancing the efficacy of treatment in thromboembolic stroke. Taurine confers a neuroprotective effect in the mechanical stroke model. This effect has not been assessed in an embolic stroke model. Here, we sought to evaluate the neuroprotective effect of taurine alone and in combination with thrombolytic therapy to investigate whether combined administration would extend the therapeutic time window without increasing the hemorrhagic transformation in a rat embolic stroke model. Rats were subjected to right embolic middle cerebral artery occlusion and then randomly assigned to the following groups: saline treatment alone at 4 h, urokinase, taurine treatment alone at 4, 6, or 8 h, and the combination of taurine and urokinase at 4, 6, or 8 h after the insult. Brain infarct volume, neurobehavioral outcome, regional cerebral blood flow, intracranial hemorrhage incidence were observed and evaluated. Posttreatment with taurine at 4 or 6 h, urokinase at 4 h or in combination at 4, 6, or 8 h significantly reduced infarct volume and improved neurobehavioral outcome. The combination treatment had better neurobehavioral outcome and smaller infarction volume than urokinase or taurine treatment alone. The clinical outcome correlated well with infarct volume. Together, the present study suggests that administration of taurine after stroke is neuroprotective, seemingly because it reduces the reperfusion damage of urokinase, leading to widen the therapeutic window for the thrombolytic effect of urokinase to 8 h. Thrombolysis can also enhance the neuroprotective effect of taurine. The reduction of inflammatory response, neuron death and inhibition of blood brain barrier (BBB) disruption may underlie the beneficial effects of combination of taurine and urokinase in the treatment of embolic stroke.

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Effect of taurine on oxidative stress and apoptosis-related protein expression in trinitrobenzene sulphonic acid-induced colitis

Cited by 53 publications

References 38 publications

Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

The effect of progesterone in the prevention of the chemically induced experimental colitis in rats

A Combined Treatment with Taurine and Intra-arterial Thrombolysis in an Embolic Model of Stroke in Rats: Increased Neuroprotective Efficacy and Extended Therapeutic Time Window

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