Effect of tanshinone on the levels of nitric oxide synthase and acetylcholinesterase in the brain of alzheimer’s disease rat model

Abstract

Clin Invest Med 2008; 31 (5): E248-E257. AbstractPurpose: To determine the influence of tanshinone on the levels of nitric oxide synthase (NOS) and acetylcholinesterase (AChE) in the brain of an Altzheimer Disease (AD) rat model and on its potential therapeutic mechanism. Methods: 100 Male Sprague Dawley rats were divided into three groups: control group, model group and tanshinone treatment group. 10 g A 1-42 was injected bilaterally into the dorsal lateral region of the dentate gyrus in the hippocampus of rats in the model and tanshinone treatment groups to prepare the AD models. 24h after modeling, tanshinone, 50mg/kg, was administered by gastric perfusion to rats in the tanshinone treatment group. Later, immunohistochemical assay and Western blot analysis were used to detect expression of neuronal NOS (nNOS) and inducible NOS (iNOS) in the rat hippocampus. Activity of AChE in each subregion (CA1~CA4) of rats' hippocampus was determined by a histochemical technique. Results: Expression of nNOS in the model group was down-regulated whereas iNOS was up-regulated. After A 1-42 injection, the number of AChE positive fibers in each subregion (CA1~CA4) of the hippocampus was decreased compared with controls. With tanshinone administration, the changes were improved to varying degrees. Conclusion:Tanshinone modulates AChE and NOS proteins concentrations in the hippocampus of AD rats. This may have therapeutic potential in AD rats.

Effects of sodium tanshinone II A sulphonate on hypoxic pulmonary hypertension in rats in vivo and on Kv2.1 expression in pulmonary artery smooth muscle cells in vitro

“…Moreover, it has obvious impact on cytokine secretion. Extraction from Radix et Rhizoma Salviae Miltriorrhizae inhibited endothelin-1 secretion and promoted nitric oxide synthesis in pulmonary arterial endothelial cells exposed to hypoxia (Jiang et al, 1997), and also reduced nitric oxide synthase (NOS) expression in Alzheimer's disease rat model (Yin et al, 2008). Previous reports showed that tanshinone reduces mean arterial pressure in renal hypertensive hamsters by activating endothelial NOS in the microcirculation (Kim et al, 2007).…”

Tanshinone IIA attenuates the cerebral ischemic injury-induced increase in levels of GFAP and of caspases-3 and -8

“…Since many neurodegenerative diseases involve cell death and inappropriately elevated levels of immune activity, it is likely that an agent such as TSA with anti-inflammatory and anti-apoptic properties, will also find application in the treatment of a broader range of age-related diseases of the nervous system. For example, a protective effect of TSA has been reported in animal models of Alzheimer's disease (Yin et al, 2008, Jiang et al, 2014.…”

Compound danshen tablet ameliorated aβ25-35-induced spatial memory impairment in mice via rescuing imbalance between cytokines and neurotrophins

“…Compound Danshen Tablet consisted of Savia miltiorrhiza , Panax Notoginseng and Borneol . Multiple main active ingredient of Compound Danshen Tablets (CDT) has been demonstrated to possess therapeutic effects against Alzheimer’s disease in animal model [15,16]. However, to the best of our knowledge, there is no available study that evaluates the effects of Compound Danshen Tablets (CDT) on neuroinflammation and neurotrophin levels in an experimental mice model of Alzheimer’s disease.…”