Compound danshen tablet ameliorated aβ25-35-induced spatial memory impairment in mice via rescuing imbalance between cytokines and neurotrophins

Abstract

BackgroundCompound Danshen Tablet (CDT), a Traditional Chinese Medicine, has recently been reported to improve spatial cognition in a rat model of Alzheimer’s disease. However, in vivo neuroprotective mechanism of the CDT in models of spatial memory impairment is not yet evaluated. The present study is aimed to elucidate the cellular mechanism of CDT on Aβ25-35-induced cognitive impairment in mice.MethodsMice were randomly divided into 5 groups: the control group (sham operated), the Aβ25-35 treated group, the positive drug group, and large and small dosage of the CDT groups, respectively. CDT was administered at a dose of 0.81 g/kg and 0.405 g/kg for 3 weeks. The mice in the positive drug group were treated with 0.4 mg/kg of Huperzine A, whereas the mice of the control and Aβ25-35 treated groups were administrated orally with equivalent saline. After 7 days of preventive treatment, mice were subjected to lateral ventricle injection of Aβ25-35 to establish the mice model of Alzheimer’s disease. Spatial memory impairment was evaluated by Morris water maze test. Choline acetyltransferase (ChAT) contents in hippocampus and cortex were quantified by ELISA. The levels of cytokines, receptor of activated protein kinase C1 (RACK1) and brain-derived neurotrophic factor (BDNF) in hippocampus were measured by RT-PCR and ELISA.ResultsThe results showed that Aβ25-35 caused spatial memory impairment as demonstrated by performance in the Morris water maze test. CDT was able to confer a significant improvement in spatial memory, and protect mice from Aβ25-35-induced neurotoxicity. Additionally, CDT also inhibited the increase of TNF-α and IL-6 level, and increased the expression of choline acetyltransferase (ChAT), receptor of activated protein kinase C1 (RACK1) and brain-derived neurotrophic factor (BDNF) in brain as compared to model mice.ConclusionThese findings strongly implicate that CDT may be a useful treatment against learning and memory deficits in mice by rescuing imbalance between cytokines and neurotrophins.

Effect of intravenous infusion of dobutamine hydrochloride on the development of early postoperative cognitive dysfunction in elderly patients via inhibiting the release of tumor necrosis factor-α

“…In the periphery, the production of TNF-α has also been confirmed as a key mediator of synaptic instability to contribute to sensory and cognitive deficits (Yang et al, 2013). In addition, many studies have shown that the inhibition of TNF-α is associated with an improvement of cognitive dysfunction Teng et al, 2014). Therefore, inflammatory mediators, such as TNF-α, may play an important role on the course of POCD development, and they may serve as new targets for the prevention of POCD after surgery.…”

Tanshinone IIA attenuates Aβ25–35-induced spatial memory impairment via upregulating receptors for activated C kinase1 and inhibiting autophagy in hippocampus

“…Amyloid β‐protein fragment 25–35 (Aβ 25–35 ) was purchased from Beijing Biosynthesis Biotechnology Co, LTD (Beijing, China). The Aβ 25–35 was dissolved in sterile saline at a concentration of 2 g/l and incubated at 37 °C for 7 days to allow for fibril formation as described previously …”

“…The MT‐200 Morris water maze (MWM) system (Chengdu TME Technology Co, Ltd, Chengdu, China) was used to assess the spatial learning and memory abilities in mice before and after the animal treatment as described previously . Twice training trails per day were conducted for four consecutive days, with an intertribal interval of 15 min.…”

The Coumarin Derivative Osthole Stimulates Adult Neural Stem Cells, Promotes Neurogenesis in the Hippocampus, and Ameliorates Cognitive Impairment in APP/PS1 Transgenic Mice

“…47,49,50) The apparatus consisted of a circular pool (120 cm diameter ×60 cm height) with a black inner wall, which was subdivided into four equal quadrants and filled with water (25°C) to the depth of 30 cm. An escape platform (10 cm diameter) was placed in one of the quadrants (the target quadrant) and submerged approximately 2 cm below the surface of the water.…”

“…investigate the effects of Ost on cognitive function of Tg mice, hippocampus-dependent learning and memory was analyzed using Morris water maze test after a six-week treatment with Ost. 49,50) Spatial learning was expressed as the latency of time spent on finding the escape platform in the water maze. 47) Significant differences among the three groups were observed (Figs.…”