Effect of Tannin Extracts on Biofilms and Attachment of Escherichia coli on Lettuce Leaves

Klug, Tâmmila Venzke; Novello, Júnia Capua de Lima; Laranja, Daniela Comparsi; Aguirre, Tanira; Rios, Alessandro de Oliveira; Tondo, Eduardo César; Santos, Rinaldo Pires dos; Bender, Renar João

doi:10.1007/s11947-016-1812-0

Cited by 17 publications

(7 citation statements)

References 34 publications

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“…Alkaloids also have an antibacterial mechanism that is almost the same as other phytochemical compounds found in all extracts of this plant such as inhibition of bacterial cell wall synthesis, bacterial metabolism, nucleic acid and protein synthesis, also disturbing the permeability of bacterial cell membrane [52,53]. In addition, plant-derived tannins have also been used as natural antibacterial substances by interfering biofilm formation, thus reducing bacterial virulence [54,55]. The antibacterial activity of all plant extracts increased linearly as the concentration of the extracts increased.…”

Section: Resultsmentioning

confidence: 97%

Natural Inhibitor of Agronomically Repellent Plant Towards Clinical Isolate of Chloramphenicol Resistant-Salmonella Typhi

Kusuma

Herawati²,

Ramdhani³

et al. 2022

Int J App Pharm

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Objective: This study was purposed to determine the most effective inhibition among those repellent plants i.e. The leaves of kirinyuh (Chromolaena odorata), kenikir (Cosmos caudatus), bandotan (Ageratum conyzoides), grass teki (Cyperus Cyperus rotundus), lemongrass (Cymbopogon citratus) and suren (Toona sureni) towards S. typhi clinical isolate. Methods: The ethanolic extracts of sixt plants were obtained by maceration method using 70% ethanol. Phytochemical screening was done using the standard methods as described by Farnsworth. The inhibition of the repellent leaves ethanolic extracts to chloramphenicol resistant-S. typhi clinical isolate assayed using the agar diffusion method and statistically analyzed by ANOVA followed by the Duncan test. The most potential plant was further determined by investigating the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) value using the microdilution test. Results: As the result, all ethanolic leaves extracts contain alkaloids, flavonoids, and tannins, except that tannins were not found in C. rotundus and A. conyzoides. However, all extracts had the activity to inhibit the growth of S. typhi. T. sureni leaves extract evidently showed the strongest inhibition with MIC value in the range of 1.5625<x≤3.125 mg/ml and the MBC value in the range of 6.25<x≤12.5 mg/ml. The ratio of MBC/MIC≤4, thus, T. sureni leaf extract may be classified as a strong bactericidal agent. Conclusion: In summary, T. sureni extract leaves achieved the most appreciable value of MIC MBC and considered as the bactericidal agent which has strong potential to be a novel anti-typhoid fever agent.

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Section: Resultsmentioning

confidence: 97%

Natural Inhibitor of Agronomically Repellent Plant Towards Clinical Isolate of Chloramphenicol Resistant-Salmonella Typhi

Kusuma

Herawati²,

Ramdhani³

et al. 2022

Int J App Pharm

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“…The generally weak to moderate antimicrobial activity of the extracts indicates that direct pathogen inhibition might not be the only reason for the successful traditional use of the plants for infections. Hence, the question arose whether the main effects may be induced by tannins which target the virulence factors, like the biofilm, instead of a direct antimicrobial effect [ 25 ]. This thus lead to the conduction of an anti-biofilm assay with gram-negative test organisms to prove the hypothesis.…”

Section: Resultsmentioning

confidence: 99%

Evaluating Tannins and Flavonoids from Traditionally Used Medicinal Plants with Biofilm Inhibitory Effects against MRGN E. coli

et al. 2022

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In the search for alternative treatment options for infections with multi-resistant germs, traditionally used medicinal plants are currently being examined more intensively. In this study, the antimicrobial and anti-biofilm activities of 14 herbal drugs were investigated. Nine of the tested drugs were traditionally used in Europe for treatment of local infections. For comparison, another five drugs monographed in the European Pharmacopoeia were used. Additionally, the total tannin and flavonoid contents of all tested drugs were analyzed. HPLC fingerprints were recorded to obtain further insights into the components of the extracts. The aim of the study was to identify herbal drugs that might be useable for treatment of infectious diseases, even with multidrug resistant E. coli, and to correlate the antimicrobial activity with the total content of tannins and flavonoids. The agar diffusion test and anti-biofilm assay were used to evaluate the antimicrobial potential of different extracts from the plants. Colorimetric methods (from European Pharmacopeia) were used for determination of total tannins and flavonoids. The direct antimicrobial activity of most of the tested extracts was low to moderate. The anti-biofilm activity was found to be down to 10 µg mL−1 for some extracts. Tannin contents between 2.2% and 10.4% of dry weight and total flavonoid contents between 0.1% and 1.6% were found. Correlation analysis indicates that the antimicrobial and the anti-biofilm activity is significantly (p < 0.05) dependent on tannin content, but not on flavonoid content. The data analysis revealed that tannin-rich herbal drugs inhibit pathogens in different ways. Thus, some of the tested herbal drugs might be useable for local infections with multi-resistant biofilm-forming pathogens. For some of the tested drugs, this is the first report about anti-biofilm activity, as well as total tannin and flavonoid content.

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“…Lettuce is often involved in foodborne outbreaks caused by pathogenic E. coli. Klug et al [ 64 ] compared the efficacy of tannin extracts and chlorine treatments on the reduction of E. coli ATCC 25922 adhering to lettuce leaves. The treatment with tannin extracts from black wattle (1%, v / v ) reduced the E. coli adhering to and under any biofilm formation on lettuce leaves and its effect was similar to that found with chlorine solutions.…”

Section: Biological Activities Of Wattle Tanninmentioning

confidence: 99%

Tannins from Acacia mearnsii De Wild. Bark: Tannin Determination and Biological Activities

Ogawa¹,

Yazaki

2018

Molecules

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The bark of Acacia mearnsii De Wild. (black wattle) contains significant amounts of water-soluble components acalled “wattle tannin”. Following the discovery of its strong antioxidant activity, a wattle tannin dietary supplement has been developed and as part of developing new dietary supplements, a literature search was conducted using the SciFinder data base for “Acacia species and their biological activities”. An analysis of the references found indicated that the name of Acacia nilotica had been changed to Vachellia nilotica, even though the name of the genus Acacia originated from its original name. This review briefly describes why and how the name of A. nilotica changed. Tannin has been analyzed using the Stiasny method when the tannin is used to make adhesives and the hide-powder method is used when the tannin is to be used for leather tanning. A simple UV method is also able to be used to estimate the values for both adhesives and leather tanning applications. The tannin content in bark can also be estimated using NIR and NMR. Tannin content estimations using pyrolysis/GC, electrospray mass spectrometry and quantitative 31P-NMR analyses have also been described. Tannins consists mostly of polyflavanoids and all the compounds isolated have been updated. Antioxidant activities of the tannin relating to anti-tumor properties, the viability of human neuroblastoma SH-SY5Y cells and also anti-hypertensive effects have been studied. The antioxidant activity of proanthocyanidins was found to be higher than that of flavan-3-ol monomers. A total of fourteen papers and two patents reported the antimicrobial activities of wattle tannin. Bacteria were more susceptible to the tannins than the fungal strains tested. Several bacteria were inhibited by the extract from A. mearnsii bark. The growth inhibition mechanisms of E. coli were investigated. An interaction between extracts from A. mearnsii bark and antibiotics has also been studied. The extracts from A. mearnsii bark inhibit the growth of cyanobacteria. Wattle tannin has the ability to inactivate α-amylase, lipase and glucosidase. In vivo experiments on anti-obesity and anti-diabetes were also reported. Several patents relating to these enzymes for anti-diabetes and anti-obesity are in the literature. In addition, studies on Acacia bark extract regarding its antitermite activities, inhibition of itching in atopic dermatitis and anti-inflammatory effects have also been reported. The growth of bacteria was inhibited by the extract from A. mearnsii bark, and typical intestinal bacteria such as E. coli, K. pneumoniae, P. vulgaris and S. marcescenes was also inhibited in vitro by extracts. Based on these results, the Acacia bark extract may inhibit not only the growth of these typical intestinal bacteria but also the growth of other types of intestinal bacteria such as Clostridium and Bacteroides, a so-called “bad bacteria”. If the tannin extract from A. mearnsii bark inhibits growth of these “bad bacteria” in vivo evaluation, the extracts might be usable as a new diet...

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Effect of Tannin Extracts on Biofilms and Attachment of Escherichia coli on Lettuce Leaves

Cited by 17 publications

References 34 publications

Natural Inhibitor of Agronomically Repellent Plant Towards Clinical Isolate of Chloramphenicol Resistant-Salmonella Typhi

Natural Inhibitor of Agronomically Repellent Plant Towards Clinical Isolate of Chloramphenicol Resistant-Salmonella Typhi

Evaluating Tannins and Flavonoids from Traditionally Used Medicinal Plants with Biofilm Inhibitory Effects against MRGN E. coli

Tannins from Acacia mearnsii De Wild. Bark: Tannin Determination and Biological Activities

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