Effect of Tannic Acid on the Protective Properties of the in situ Formed Pellicle

Hertel, Susann; Pötschke, Sandra; Basche, Sabine; Delius, Judith; Hoth-Hannig, Wiebke; Hannig, Matthias; Hannig, Christian

doi:10.1159/000451036

Cited by 35 publications

(36 citation statements)

References 48 publications

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“…Tannic acid or polyphenols in general are known for their antibacterial and anti-adherent properties [ 24 , 28 ]. Tannins can precipitate and inhibit proteins, form chelate complexes and change pellicle structures [ 21 , 22 , 23 , 24 ]. The tannic acid used in the present study was obtained from gall apples of Quercus infectoria oak.…”

Section: Discussionmentioning

confidence: 99%

“…By inhibiting the enzyme glycosyl-transferase, which is used by bacteria to adhere to the pellicle, tannins are able to inhibit bacterial adherence [ 22 , 23 ]. The effect on bacterial adherence was researched recently, where a mouthwash containing tannic acid showed an anti-adherent effect even eight hours after an initial application, speaking for a long-lasting effect [ 24 ]. Tannins alter the ultrastructure of pellicles, resulting in thicker and electron-denser pellicles, as shown with transmission electron microscopy [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Experimental Agents Containing Tannic Acid or Chitosan on the Bacterial Biofilm Formation in Situ

Schestakow

Hannig

2020

Biomolecules

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Chitosan and tannic acid are known for their antibacterial properties. In the present in-situ study, their antibacterial and anti-adherent effects on biofilm formation on enamel were investigated. Six subjects carried upper jaw splints with bovine enamel specimens, allowing in-situ biofilm formation. During the two-day trial, subjects rinsed with experimental solutions that contained either chitosan, tannic acid (pH = 2.5), tannic acid (pH = 7) or hydrochloric acid. Water served as the negative and chlorhexidine as the positive control. Rinsing occurred four or five times following two different rinsing protocols to investigate both the immediate and long-lasting effects. After 48 h of intraoral exposure, the dental plaque was stained with LIVE/DEAD® BacLight, and fluorescence micrographs were evaluated by using the software ImageJ. The results were verified by scanning electron microscopy. Rinsing with chitosan resulted in little immediate antibacterial and anti-adherent effects but failed to show any long-lasting effect, while rinsing with tannic acid resulted in strong immediate and long-lasting effects. Except for a slightly lower antibacterial effect, the neutral solution of tannic acid was as good as the acidic solution. Hydrochloric acid showed neither an antibacterial nor an anti-adherent effect on dental biofilm formation. Experimental solutions containing tannic acid are promising anti-biofilm agents, irrespective of the pH values of the solutions. Chitosan, on the other hand, was not able to prevent biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Experimental Agents Containing Tannic Acid or Chitosan on the Bacterial Biofilm Formation in Situ

Schestakow

Hannig

2020

Biomolecules

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“…To the best knowledge of the authors, a systematic analysis of ultrastructural modifications of the in situ pellicle by different fluoride components has so far never been performed. By contrast, the oralprophylactic properties of several functional agents have already been correlated with an alteration of the pellicle’s ultrastructure 29 – 31 . In general, none of the applied fluoride containing mouthrinses without stannous ions appeared to notably affect the thickness or electron density of the in situ formed 30 min pellicle, thus a considerable impact of fluorides on the pellicle’s ultrastructure can be questioned.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro , mouthrinses with fluorides and particularly if supplemented by stannous ions appeared to modify the quantity and the composition of the pellicle proteins accumulated at the enamel surface 8 , 28 . This could enhance the pellicle’s resistance against acid-derived dissolution and could furthermore lead to a modified bacterial colonization 29 . However, so far, there is no published data about fluoride-associated effects on the in situ pellicle’s ultrastructure 30 .…”

Section: Introductionmentioning

confidence: 99%

Impact of customary fluoride rinsing solutions on the pellicle’s protective properties and bioadhesion in situ

Kensche

Kirsch

Mintert

et al. 2017

Sci Rep

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This study investigated the impact of customary fluoride based mouthrinses on the ultrastructure and the functional properties of the in situ pellicle, considering the prevention of erosion (8 volunteers) and initial biofilm formation (12 volunteers). Bovine enamel slabs were carried intraorally. After 1 min of pellicle formation, the subjects rinsed with elmex Kariesschutz (A), Dontodent Med Care (B), meridol (C) or elmex Zahnschmelzschutz Professional (D) for 1 min. In situ pellicle formation was continued up to 30 min/8 h before processing the slabs in vitro. Erosion was simulated by incubating the specimens in HCl (pH 3.0, 2.3, 2.0) for 120 s, measuring the kinetics of calcium/phosphate release photometrically; representative samples were evaluated by TEM and EDX. Bacterial adhesion was visualized fluorescence microscopically (DAPI/BacLight). Native enamel slabs or physiological pellicle samples served as controls. All investigated mouthrinses enhanced the erosion preventive pellicle effect in dependence of the pH-value. A significant decrease of Ca/P release at all pH values was achieved after rinsing with D; TEM/EDX confirmed ultrastructural pellicle modifications. All mouthrinses tendentially reduced bacterial adherence, however not significantly. The mouthrinse containing NaF/AmF/SnCl2 (D) offers an effective oral hygiene supplement to prevent caries and erosion.

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“…Bacterial adhesion can be disrupted when the molecular composition and physicochemical properties of the pellicle are altered [8]. Modification of the pellicle by plant extracts or ingredients of natural products, such as tannic acid and propolis, is a safe and cost-effective approach to prevent bacterial adhesion [9,10].…”

Section: Introductionmentioning

confidence: 99%

Casein phosphopeptide acts synergistically with fluoride to inhibit initial adhesion of Streptococcus mutans to the saliva-coated hydroxyapatite disc

Wang¹,

Liu²,

Zhou³

et al. 2020

Preprint

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Background Recent preventive strategies for dental caries focus on targeting the mechanisms underlying biofilm formation, including inhibiting bacterial adhesion. A promising approach to prevent bacterial adhesion is to modify the composition of acquired salivary pellicle. This in vitro study investigated the effect and possible underlying mechanism of pellicle modification by casein phosphopeptide (CPP) on Streptococcus mutans (S. mutans) initial adhesion, and the impact of fluoride on the efficacy of CPP.Methods The salivary pellicle-coated hydroxyapatite (s-HA) disc was modified by 2.5% CPP or 2.5% CPP supplemented with 900 ppm fluoride solutions. After cultivation of S. mutans, the adherent bacteria were visualized by scanning electron microscopy (SEM) and quantitatively evaluated using the plate count method. Confocal laser scanning microscopy (CLSM) was used to evaluate the proportions of total and dead S. mutans. The concentrations of total, free, and bound calcium and fluoride in CPP and fluoride-doped CPP solutions were determined. The water contact angle and zeta potential of s-HA with and without modification were measured. The data were statistically analyzed using one-way ANOVA followed by a Turkey post hoc multiple comparison test.Results Initial adhesion of S. mutans to s-HA was inhibited in response to pellicle modification by CPP and fluoride-doped CPP, and the latter was more efficient. CLSM analysis showed that the proportion of dead S. mutans did not differ between the groups. Water contact angle and zeta potential decreased after pellicle modification, and both were lowest in the fluoride-doped CPP group.Conclusions Pellicle modification by CPP inhibited S. mutans initial adhesion to s-HA, possibly by reducing hydrophobicity and negative charge on the s-HA surface, and incorporating fluoride into CPP enhanced its anti-adhesion effect.

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Effect of Tannic Acid on the Protective Properties of the in situ Formed Pellicle

Cited by 35 publications

References 48 publications

Effects of Experimental Agents Containing Tannic Acid or Chitosan on the Bacterial Biofilm Formation in Situ

Effects of Experimental Agents Containing Tannic Acid or Chitosan on the Bacterial Biofilm Formation in Situ

Impact of customary fluoride rinsing solutions on the pellicle’s protective properties and bioadhesion in situ

Casein phosphopeptide acts synergistically with fluoride to inhibit initial adhesion of Streptococcus mutans to the saliva-coated hydroxyapatite disc

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