Impact of customary fluoride rinsing solutions on the pellicle’s protective properties and bioadhesion in situ

Abstract

This study investigated the impact of customary fluoride based mouthrinses on the ultrastructure and the functional properties of the in situ pellicle, considering the prevention of erosion (8 volunteers) and initial biofilm formation (12 volunteers). Bovine enamel slabs were carried intraorally. After 1 min of pellicle formation, the subjects rinsed with elmex Kariesschutz (A), Dontodent Med Care (B), meridol (C) or elmex Zahnschmelzschutz Professional (D) for 1 min. In situ pellicle formation was continued up to 30 min/8 h before processing the slabs in vitro. Erosion was simulated by incubating the specimens in HCl (pH 3.0, 2.3, 2.0) for 120 s, measuring the kinetics of calcium/phosphate release photometrically; representative samples were evaluated by TEM and EDX. Bacterial adhesion was visualized fluorescence microscopically (DAPI/BacLight). Native enamel slabs or physiological pellicle samples served as controls. All investigated mouthrinses enhanced the erosion preventive pellicle effect in dependence of the pH-value. A significant decrease of Ca/P release at all pH values was achieved after rinsing with D; TEM/EDX confirmed ultrastructural pellicle modifications. All mouthrinses tendentially reduced bacterial adherence, however not significantly. The mouthrinse containing NaF/AmF/SnCl2 (D) offers an effective oral hygiene supplement to prevent caries and erosion.

Effect of fluoride mouthrinses and stannous ions on the erosion protective properties of the in situ pellicle

“…Recent in vitro and in situ investigations have shown, that the application of different fluoride containing preparations might have an influence on the pellicle layer or the pellicle’s ultrastructure, respectively 7,9 . It has been suggested, that the fluoride bound cations interfere with the adsorption processes of salivary proteins at the tooth surface 7,9 . Ideally, this would promote the formation of a more acid-resistant pellicle layer.…”

“…Ideally, this would promote the formation of a more acid-resistant pellicle layer. In situ investigations have yielded corresponding results after simple mouthrinses with a sodium fluoride (NaF)-, amine fluoride (AmF)- and stannous chloride (SnCl 2 ) containing customary preparation 7 . In comparison, a purely NaF-based mouthrinse appeared to facilitate protein re-adsorption to the demineralised enamel surface after acid exposure.…”

“…In comparison, a purely NaF-based mouthrinse appeared to facilitate protein re-adsorption to the demineralised enamel surface after acid exposure. This might rather contribute to a remineralising effect 7,10,11 . Especially stannous ions significantly improved the erosion preventive effect of fluoride containing prophylactic measures in vitro and in situ 7,12–16 .…”

“…This might rather contribute to a remineralising effect 7,10,11 . Especially stannous ions significantly improved the erosion preventive effect of fluoride containing prophylactic measures in vitro and in situ 7,12–16 . One explanation could be the strengthening of the pellicle’s acid resilience 7 .…”

Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model

“…Biofilm in situ models offer a promising and standardizable option for obtaining biofilm samples over strictly defined periods of time 7 , 13 . These in situ models can be used to investigate initial bioadhesion processes, basic microbiological and molecular genetic questions, as well as the influences of food and oral hygiene products 13 – 15 . Depending on the requirements, glass, polymers, dental materials or dental hard substances can be used as the basic substrate 13 , 16 – 18 .…”