Effect of Synergists on Deltamethrin Resistance in the Common Bed Bug (Hemiptera: Cimicidae)

González-Morales, María A; Romero, Alvaro

doi:10.1093/jee/toy376

Cited by 25 publications

(7 citation statements)

References 45 publications

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“…Insecticide resistance in bed bugs has been evaluated using technical grade insecticides [ 4 , 5 , 6 , 7 ] and commercial insecticide products and formulations [ 8 , 9 , 10 , 11 ]. Bed bugs have been found resistant to several insecticide classes that are commonly incorporated in formulated products, such as pyrethroids [ 11 , 12 , 13 ], neonicotinoids [ 4 , 14 ], organophosphates [ 15 , 16 , 17 ], and pyrroles [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Several bioassay methods have been used in resistance testing. For instance, dose-response assays with topical application [ 4 , 6 , 7 , 18 ], concentration-response assays with a surface contact method [ 10 , 19 , 20 , 21 ], and time-response assays with a surface contact method [ 8 , 11 , 17 , 22 ], or direct spraying [ 17 ]. For laboratory evaluations of commercial insecticide products and formulations, a time-response assay [ 8 , 11 , 23 ] and endpoint assay [ 9 , 24 ] have been commonly used.…”

Section: Introductionmentioning

confidence: 99%

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Influences of Exposure Time and Mortality Assessment Interval on Bioassay Results of Insecticide-Resistant Tropical Bed Bugs (Hemiptera: Cimicidae)

Leong

Singham

Shu-Chien

et al. 2020

Insects

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We evaluated the influences of insecticide exposure time and mortality assessment intervals on the bioassay results of three insecticide-resistant tropical bed bug Cimex hemipterus (F.) populations (Madam Mo, Tanjong Tokong, and Green Lane). This was achieved using the surface contact method and tested with six commercial insecticide products: Tandem, Temprid SC, Pesguard FG161, Sumithrin, Sumithion, and Phantom applied at label rate on glass petri dishes. Six exposure times (5 min, 10 min, 30 min, 1 h, 4 h, and continuous exposure for 4 or 14 days for Phantom) were tested. A susceptible common bed bug Cimex lectularius L. strain (Monheim) was used as the reference strain as no susceptible C. hemipterus strain exists. In treatment with Temprid SC, the Tanjong Tokong strain showed significantly higher KT50 values at 5, 10, and 30 min exposures than 1 h, 4 h, and continuous exposures. When all resistant populations were evaluated at continuous exposure to Sumithion, they demonstrated moderate resistance levels (RR50 ranged from 6.0 to 7.9), while KT50 and KT95 of other shorter exposure times failed to be generated due to low knockdown rate. Higher mortalities were observed in Tanjong Tokong and Green Lane strains when tested at longer exposure times with Temprid SC, Pesguard FG161, Sumithrin, Sumithion, and Phantom. Better killing effect was observed in the treatment with Temprid SC (Tanjong Tokong and Green Lane strains), Pesguard FG161 (Tanjong Tokong and Green Lane strains), Sumithrin (all C. hemipterus strains), Sumithion (all C. hemipterus strains), and Phantom (all strains tested) at longer mortality assessment intervals. We demonstrated that insecticide exposure time and mortality assessment interval could potentially affect outcomes of product performance evaluations, resulting in underestimation or overestimation of insecticide resistance levels in field populations.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influences of Exposure Time and Mortality Assessment Interval on Bioassay Results of Insecticide-Resistant Tropical Bed Bugs (Hemiptera: Cimicidae)

Leong

Singham

Shu-Chien

et al. 2020

Insects

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“…Cross-resistance with DDT, which was previously widely used to control bed bug infestations, may have also contributed to high levels of resistance in modern day bed bugs [ 17 ]. Enhancement of the detoxification enzymes [ 21 , 22 , 23 , 24 ] and insensitivity on target sites ( kdr mutation) [ 9 , 19 , 25 , 26 ] are the most studied mechanisms for pyrethroid resistance. Further, cuticular modification was also confirmed in a pyrethroid-resistant bed bug strain [ 27 ] which may reduce the penetration of insecticide into insect’s body.…”

Section: Introductionmentioning

confidence: 99%

Insecticide Resistance of Cimex lectularius L. Populations and the Performance of Selected Neonicotinoid-Pyrethroid Mixture Sprays and an Inorganic Dust

Ranabhat

Wang

2023

Insects

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Insecticide resistance is one of the factors contributing to the resurgence of the common bed bug, Cimex lectularius L. This study aimed to profile the resistance levels of field-collected C. lectularius populations to two neonicotinoids and one pyrethroid insecticide and the performance of selected insecticide sprays and an inorganic dust. The susceptibility of 13 field-collected C. lectularius populations from the United States to acetamiprid, imidacloprid, and deltamethrin was assessed by topical application using a discriminating dose (10 × LD90 of the respective chemical against a laboratory strain). The RR50 based on KT50 values for acetamiprid and imidacloprid ranged from 1.0–4.7 except for the Linden 2019 population which had RR50 of ≥ 76.9. Seven populations had RR50 values of > 160 for deltamethrin. The performance of three insecticide mixture sprays and an inorganic dust were evaluated against three C. lectularius field populations. The performance ratio of Transport GHP (acetamiprid + bifenthrin), Temprid SC (imidacloprid + β-cyfluthrin), and Tandem (thiamethoxam + λ-cyhalothrin) based on LC90 were 900–2017, 55–129, and 100–196, respectively. Five minute exposure to CimeXa (92.1% amorphous silica) caused > 95% mortality to all populations at 72 h post-treatment.

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“…The copyright holder for this preprint this version posted October 1, 2021. ; https://doi.org/10.1101/2021.09. 29.462303 doi: bioRxiv preprint tributyl phosphorotrithioate (DEF), triphenyl phosphate (TPP), and diethyl maleate (DEM) are used as synergists for other classes of insecticides [17][18][19]. However, PBO is the most popularly used insecticide synergist [16,17].…”

Section: Introductionmentioning

confidence: 99%

Emerging mosquito resistance to piperonyl butoxide-synergized pyrethroid insecticide and its mechanism

Zhou

Jeang

et al. 2021

Preprint

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Piperonyl butoxide (PBO)-synergized pyrethroid products are widely available for the control of pyrethroid-resistant mosquitoes. To date, no study has formally examined mosquito resistance to PBO-synergized insecticides. We used Culex quinquefasciatus as a model mosquito examined the insecticide resistance mechanisms of mosquitoes to PBO-synergized pyrethroid using modified World Health Organization tube bioassays and biochemical analysis of metabolic enzyme expressions prior- and post-PBO exposure. We measured mosquito mortalities and metabolic enzyme expressions in mosquitoes with/without pre-exposure to different PBO concentrations and exposure durations. We found that field Culex quinquefasciatus mosquitoes were resistant to all insecticides tested, including PBO-synergized pyrethroids (mortality ranged from 3.7±4.7% to 66.7±7.7%), except malathion. Field mosquitoes had elevated levels of carboxylesterase (COE, 3.8-fold) and monooxygenase (P450, 2.1-fold) but not glutathione S-transferase (GST) compared to susceptible mosquitoes. When the field mosquitoes were pre-exposed to 4% PBO, the 50% lethal concentration of deltamethrin was reduced from 0.22% to 0.10%, compare to 0.02% for susceptible mosquitoes. Knockdown resistance gene mutation (L1014F) rate was 62% in field mosquitoes. PBO pre-exposure suppressed P450 enzyme expression levels by 25~34%, GST by 11%, and had no impact on COE enzyme expression. Even with the optimal PBO concentration and exposure duration, field mosquitoes had significantly higher P450 enzyme expression levels after PBO exposure compared to laboratory controls. These results demonstrate that PBO alone may not be enough to control highly pyrethroid resistant mosquitoes due to the multiple resistance mechanisms. Mosquito resistance to PBO-synergized insecticide should be closely monitored.

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Effect of Synergists on Deltamethrin Resistance in the Common Bed Bug (Hemiptera: Cimicidae)

Cited by 25 publications

References 45 publications

Influences of Exposure Time and Mortality Assessment Interval on Bioassay Results of Insecticide-Resistant Tropical Bed Bugs (Hemiptera: Cimicidae)

Influences of Exposure Time and Mortality Assessment Interval on Bioassay Results of Insecticide-Resistant Tropical Bed Bugs (Hemiptera: Cimicidae)

Insecticide Resistance of Cimex lectularius L. Populations and the Performance of Selected Neonicotinoid-Pyrethroid Mixture Sprays and an Inorganic Dust

Emerging mosquito resistance to piperonyl butoxide-synergized pyrethroid insecticide and its mechanism

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