1988
DOI: 10.1530/jrf.0.0840611
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Effect of swainsonine on rat epididymal glycosidases

Abstract: Summary. Each epididymis of control and swainsonine-fed rats (5 \g=m\g/mldrinking water) was divided into 5 segments, and tissue, spermatozoa and sperm-free supernatants were prepared from each segment. When levels of 3 lysosomal glycosidases and total protein were determined, the proximal cauda contained the greatest concentration of each glycosidase. The specific-activity profile for \g=b\-glucuronidase and \g=b\-galactosidase was similar in swainsonine-fed and control rats. However, the concentration of \g=… Show more

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Cited by 19 publications
(15 citation statements)
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“…The supernatant obtained after centrifugation was assayed for PNP-mannosidase/[3H]Mang-mannosidase activities. Similar protocol has been described in our publications (36,39,41,44).…”
Section: Immunoprecipitation Studiesmentioning
confidence: 81%
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“…The supernatant obtained after centrifugation was assayed for PNP-mannosidase/[3H]Mang-mannosidase activities. Similar protocol has been described in our publications (36,39,41,44).…”
Section: Immunoprecipitation Studiesmentioning
confidence: 81%
“…Highly specific polyclonal antibody (IgG) against rat epididymai c~-D-mannosidase was prepared as reported (44). This antibody has been shown to cross-react with the rat liver lysosomal c~-v-mannosidase (44) and rat sperm acrosomal ot-D-mannosidase (36). Anti-rat liver cytosotic c~-D-mannosidase prepared as described (35) was a gift from Dr. Oscar Toaster of this university.…”
Section: Methodsmentioning
confidence: 99%
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