Effect of Surface Topography and Bioactive Properties on Early Adhesion and Growth Behavior of Mouse Preosteoblast MC3T3-E1 Cells

Li, Na; Chen, Gang; Liu, Jue; Xia, Yang; Chen, Hanbang; Tang, Hui; Zhang, Feimin; Gu, Ning

doi:10.1021/am5047944

Cited by 45 publications

(39 citation statements)

References 57 publications

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“…This concept has been experimentally supported using nanopatterned substrates that mimic topographic features encountered in the native extracellular basement. 5,7,14 Cells were cultured for 24 h on various nanopatterned substrates with different groove widths, and their morphological changes were observed. Cells showed an altered morphology dependent on not only the presence of nanopatterning, but also on the ratio of ridge and groove dimensions of these patterns (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Combined Effects of Substrate Topography and Stiffness on Endothelial Cytokine and Chemokine Secretion

Jeon¹,

Tsui

Jang³

et al. 2015

ACS Appl. Mater. Interfaces

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Endothelial physiology is regulated not only by humoral factors but also by mechanical factors such as fluid shear stress and the underlying cellular matrix microenvironment. The purpose of the present study was to examine the effects of matrix topographical cues on the endothelial secretion of cytokines/chemokines in vitro. Human endothelial cells were cultured on nanopatterned polymeric substrates with different ratios of ridge to groove widths (1:1, 1:2, and 1:5) and with different stiffnesses (6.7 MPa and 2.5 GPa) in the presence and absence of 1.0 ng/mL TNF-α. The levels of cytokines/chemokines secreted into the conditioned media were analyzed with a multiplexed bead-based sandwich immunoassay. Of the nano-patterns tested, the 1:1 and 1:2 type-patterns were found to induce the greatest degree of endothelial cell elongation and directional alignment. The 1:2 type nanopatterns lowered the secretion of inflammatory cytokines such as IL-1β, IL-3 and MCP-1, compared to unpatterned substrates. Additionally, of the two polymers tested, it was found that the stiffer substrate resulted in significant decreases in the secretion of IL-3 and MCP-1. These results suggest that substrates with specific extracellular nanotopographical cues or stiffnesses may provide anti-atherogenic effects like those seen with laminar shear stresses by suppressing the endothelial secretion of cytokines and chemokines involved in vascular inflammation and remodeling.

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Section: Resultsmentioning

confidence: 99%

Combined Effects of Substrate Topography and Stiffness on Endothelial Cytokine and Chemokine Secretion

Jeon¹,

Tsui

Jang³

et al. 2015

ACS Appl. Mater. Interfaces

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“…Moreover, the PDOP coating can obviously increase the surface free energy of materials. Investigations have demonstrated that the PDOP coating has a binding effect on the extracellular matrix protein from the culture medium, which enhances the adhesion and proliferation of cells . In addition, the activity of MC3T3‐E1 cells on TiO 2 /PDOP/GA samples was decreased compared with that on TiO 2 /PDOP.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of Chitosan‐18β‐Glycyrrhetinic Acid Modified Titanium Implants with Nanorod Arrays for Suppression of Osteosarcoma Growth and Improvement of Osteoblasts Activity

Zhang

Cheng

Gong

et al. 2017

Adv Funct Materials

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Surgery has been combined with chemotherapy to treat osteosarcoma. However, the recovery rate of osteosarcoma patients is still low. To prevent post-operative recurrence of the osteosarcoma, developing an alternative biomaterial is essential. 18β-Glycyrrhetinic acid (GA) has shown potential anticancer activity in various malignancies. Here it is proposed that GA can induce osteosarcoma cell apoptosis, and a polydopamine-mediated titanium oxide nanorod (TiO 2 NR) surface is functionalized by covalently grafting the chitosan-18β-glycyrrhetinic acid conjugate (CS-GA). In vitro and in vivo biological tests indicate that the CS-GA modified surface shows significant antiproliferation and apoptosis in osteosarcoma cells (MG63). Additionally, this modified surface with nanoarray structure stimulation and chitosan supplementation significantly promotes osteoblast (MC3T3-E1) adhesion and proliferation in vitro. This dual-functional, Ti-based implant with balanced antitumor and biocompatibility properties represents an effective strategy for the surgical treatment of osteosarcoma.

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“…Thereby a thin polydopamine film is formed that exhibits reactivity towards nucleophiles, such as thiols and amines. 4,12,13 Polydopamine modified materials can also be used to immobilize extracellular matrix (ECM) proteins, such as collagen type I 14 or serum proteins. 13 To create polydopamine coatings on biomaterials, a large amount of dopamine is needed.…”

Section: Introductionmentioning

confidence: 99%

Supramolecular surface functionalization via catechols for the improvement of cell–material interactions

et al. 2017

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Optimization of cell-material interactions is crucial for the success of synthetic biomaterials in guiding tissue regeneration. To do so, catechol chemistry is often used to introduce adhesiveness into biomaterials. Here, a supramolecular approach based on ureido-pyrimidinone (UPy) modified polymers is combined with catechol chemistry in order to achieve improved cellular adhesion onto supramolecular biomaterials. UPy-modified hydrophobic polymers with non-cell adhesive properties are developed that can be bioactivated via a modular approach using UPy-modified catechols. It is shown that successful formulation of the UPy-catechol additive with the UPy-polymer results in surfaces that induce cardiomyocyte progenitor cell adhesion, cell spreading, and preservation of cardiac specific extracellular matrix production. Hence, by functionalizing supramolecular surfaces with catechol functionalities, an adhesive supramolecular biomaterial is developed that allows for the possibility to contribute to biomaterial-based regeneration.

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Effect of Surface Topography and Bioactive Properties on Early Adhesion and Growth Behavior of Mouse Preosteoblast MC3T3-E1 Cells

Cited by 45 publications

References 57 publications

Combined Effects of Substrate Topography and Stiffness on Endothelial Cytokine and Chemokine Secretion

Combined Effects of Substrate Topography and Stiffness on Endothelial Cytokine and Chemokine Secretion

Fabrication of Chitosan‐18β‐Glycyrrhetinic Acid Modified Titanium Implants with Nanorod Arrays for Suppression of Osteosarcoma Growth and Improvement of Osteoblasts Activity

Supramolecular surface functionalization via catechols for the improvement of cell–material interactions

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