Effect of sunlight and artificial light on micropropagation of potato (Solanum tuberosum L.) plantlets

Abstract

Fluorescent lamps (artificial light) and sunlight were used as lighting source in present study to identify feasibility of using sunlight in plant tissue culture laboratory. In vitro regenerated nodal segments of the cardinal variety of potato (Solanum tuberosum L.) were inoculated in MS medium without any growth regulator for shoot and root development. After 30 days of culture in an average temperature at 21.94±0.21°C, relative humidity 39.35±0.75%, various morphological responses i.e. plantlet height, diameter of plantlet, number of nodes per plantlet, inter node distance, number of leaves per plantlet, fresh and dry weight of plantlets , number of root, length of root, growth rate etc. were recorded. The average light intensity at sunlight and artificial treatment was observed at 4805.5±326.54 lux and 3484±84.44 lux, respectively. All the growth factors performed better result in sunlight treatment than those of artificial one except average number of nodes and leaves. The average height of plantlet at sunlight was 53.33±3.32 mm which was greater than that of artificial light 51.67±2.15 mm. Fresh weight and dry weight are two important morphological factors for plantlet and in case of fresh weight that was always greater 0.49±0.097 in sunlighted plantlet and 0.21±0.026 in artificial one. In case of dry weight the average weight 0.08±0.016 was greater in sunlighted plantlet than that of 0.03±0.004 in artificial one. Number of roots, root length and growth rate of the plantlets observed higher in sunlighted plantlets compared to artificial light. Moreover, the sunlighted plantlets were healthier, vigorous and strong which helped plants to establish in net house easily. Considering the cost of electricity consumption, from a laboratory area of 20.47 m 2 BD Taka 63% could be saved in each month by using sunlight. As low cost options of energy saving in tissue culture laboratory using sunlight would be feasible and environmentally friendly technology for commercial point of view in Bangladesh.

Effects of Light Intensity on in Vitro Regeneration Of Stevia Rebaudiana Bertoni, Bacopa Monnieri L. And Solanum Tuberosum L.

“…Table 1 revealed that poor health and less number of multiple shoots were regenerated at 210 and 500 lx light intensity. Rehana et al (2018) observed the effect of average light intensity at sunlight and artificial florescence light treatment at 4805.5 ± 326.54 lx and 3484 ± 84.44 lx, respectively towards in vitro growth of S. tuberosum. They mentioned that all the growth factors performed better result in sunlight treatment than those of artificial one except average number of nodes and leaves.…”

Influencia de las condiciones de iluminación sobre el establecimiento in vitro de yemas de Solanum tuberosum L. var. Cecilia.

“…Mientras que, otros autores señalaron que todos los fatores de crecimiento medibles a excepción del número de hojas y nudos obtuvieron los mejores resultados al mantener los explantes por 30 días expuestos a condiciones de luz solar (con un fotoperiodo de 16 horas luz y 6 horas de oscuridad) 19 . También se menciona que el fotoperiodo de 16 horas luz favorece la regeneración in vitro y establecimiento de brotes de S. tuberosum 20 .…”

Low Cost Tissue Culture Technologies in Vegetables: A Review

“…From this research no differences were found between yield production of micropropagated plants grown under control and non-controlled conditions. The effect of natural sunlight on potato micropropagation was studied and the results were compared with the cultures produced through artificial light [81]. It was found that all the growth factors gave better result in sunlight treatment than those of artificial one except average number of nodes and leaves.…”