Influencia de las condiciones de iluminación sobre el establecimiento in vitro de yemas de Solanum tuberosum L. var. Cecilia.

Abstract

In vitro, micropropagation is an essential biotechnological tool for the mass production of plants. Several factors influence in vitro plant development, such as the culture medium, phytohormones, and illumination conditions. The present work aimed to evaluate the effect of illumination conditions on the in vitro establishment of buds of S. tuberosum Var. Cecilia. Previously disinfected tuber shoots were sown in a culture medium composed of MS + 20 g.L⁻¹ sucrose + 7 g.L⁻¹ agar and exposed to different illumination conditions. The number of nodes, the number of leaves, the number of shoots, plant height, percentage of establishment and percentage of contamination were determined. At 30 days, total fluorescent white light and 12 h natural light + 12 h darkness showed a greater plant height, while the lighting conditions showed no effect on the number of nodes, number of leaves, number of shoots per plant, percentage of establishment and percentage of contamination. Based on the results of the present work, it was concluded that both total white fluorescent light and 12 h natural light + 12 h darkness favored in vitro establishment. Keywords: darkness, growth, light, sprouts.