Effect of sugar/osmotica levels on in vitro microtuberization of potato (Solanum tuberosum L.)

Motallebi-Azar, Alireza; Kazemiani, Samaneh; Yarmohamadi, Fahimeh

doi:10.3103/s1068367413020146

Cited by 10 publications

(9 citation statements)

References 9 publications

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“…Potato (Solanum tuberosum L.) tubers are formed under short days, so microtubers are induced under short day or continuous darkness during culture conditions (Altindal & Karadoğan, 2010;Aslam et al, 2011;Dhital & Lim, 2012;Hoque, 2010;Hussain et al, 2006;Motallebi-Azar et al, 2013;Servet et al, 2000). Microtubers provided a valuable tool for study of genetic transformation (Gargantini et al, 2009;Si et al, 2008), which was followed by regeneration of plants from in vitro grown microtubers (Ishida et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…Since Barker (1953) first used etiolated sprouts to induce potato tubers in a medium containing 80g/l sucrose, high sucrose ranging from 60 to 90g/l and different explants including apical/uppermost nodal sections of the shoot and nodal segments/cuttings were used to induce in vitro tubers under a short photoperiod from 8 to 10h or in the dark (Altindal & Karadoğan, 2010;Aslam et al, 2011;Dhital & Lim, 2012;Hoque 2010;Hussain et al, 2006;Motallebi-Azar et al, 2013;Servet et al, 2000). Wang & Hu (1982) and Ewing & Struik (1992) considered sucrose may be the most critical stimulus for potato microtuber production in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Physiological Responses to High Exogenous Sucrose in Tuber Induction of Potato in Vitro

He¹,

Meng²

2016

Turkish Journal of Field Crops

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Microtubers are valuable tools to be used in the researches of potato. Among the factors sucrose is the most critical stimulus for potato microtuber production in vitro. The aim of our study was to define whether only sucrose was enough to induce the tuber and how it impacted on physiological responses in potato in vitro. Among sucrose concentrations chosen 80 and 100g/l sucrose could induce maximum tubers in three cultivars of Solanum tuberosum L., Atlantic, Desiree and 851 in vitro at 22 o C under 16-h photoperiod. Meanwhile tuber induction percentage showed no difference in MS with 80 and 100g/l sucrose among 0, 16 and 24-h photoperiod and cuttings with no nodes induced no tubers in cv. Atlantic. Therefore, 80 or 100g/l of exogenous sucrose concentrations and cuttings with node were essential to in vitro tuber induction in potato. The endogenous sucrose content and acid invertase in induced cuttings were higher than corresponding tubers and dramatically increased with the increase of sucrose concentration in MS. Starch content was completely opposite to endogenous sucrose content and decreased with the increase of sucrose concentration in MS. Sucrose synthase (Susy) and sucrose phosphate synthase (SPS) accumulated only in tubers, but no significant differences of Susy and SPS were between 80g/l and 100g/l sucrose. In conclusion, high levels of exogenous sucrose resulted in a dramatic high gradient of endogenous sucrose content between inducing cuttings and the corresponding tubers, which was beneficial to trigger differential activity expression of sugar metabolizing enzymes and to accumulate starch in tubers in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physiological Responses to High Exogenous Sucrose in Tuber Induction of Potato in Vitro

He¹,

Meng²

2016

Turkish Journal of Field Crops

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“…The main characteristics of microtubers produced through the in vitro culture technique include the production of disease-free and high-quality seeds, whose greater robustness and ease of use make them suitable explants for automated propagation systems (Motallebi-Azar, Kazemiani, & Yarmohamadi, 2013). Similarly, they facilitate the maintenance and exchange of genetic material, given that small samples can be stored and subsequently shipped (virus-free) in aseptic conditions, even to countries with strict phytosanitary regulations (Dobránszki et al, 2008;López-Delgado, Sánchez-Rojo, Mora-Herrera, & Martínez-Gutierrez, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha

García-García

Azofeifa-Bolaños

Solano-Campos

et al. 2019

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This investigation was carried out to evaluate the effect of two cytokinins: 6-benzilaminopurine (BAP) (6.5 mg l-1) and kinetin (K) (2.5 mg l-1), as well as the growth inhibitor abscisic acid (ABA) (1.0 mg l-1) on the in vitro tuberization capacity of two potato varieties: Atlantic and Alpha. The basal culture medium MS (1962) was used as a control. The responses were different between varieties. In cv. Atlantic, the analysis of the number (NM), weight (WM) and diameter (DM) of microtubers indicated that the addition of growth regulators did not affect induction and development of microtubers. However, when BAP was used, a non-significant increment of 41 % was observed in the number of the microtubers compared to the control treatment, from 2.6 to 4.4. The addition of cytokinins and ABA to the medium did not have a significant impact on the development of microtubers. In cv. Alpha the cytokinins used without ABA increased the number of microtubers, which were larger and heavier than those of the control treatment. In this variety, ABA significantly reduced the values of the NM, WM and DM variables. The exogenous action of cytokinins in the culture medium is likely to have caused an endogenous hormonal imbalance in the Atlantic and Alpha genotypes which interfered with their innate microtuberization ability, a result that was even more evident for cv. Alpha, which showed the need to continue optimizing protocols of genotype-specific systems in potato tissue culture to increase yield and seed quality.

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“…Microtubers, which are potato tubers produced by in vitro culture techniques, show many advantages that make them ideal propagules to produce high-quality seeds (Dhital & Lim, 2012;Motallebi, Kazemiani, & Yarmohamadi, 2013). First of all, disease-free, high-quality potato seeds can be obtained; and, compared to other propagation techniques, microtubers are more robust and easier to handle, which favors automatic planting processes (Dhital & Lim, 2012;Motallebi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…First of all, disease-free, high-quality potato seeds can be obtained; and, compared to other propagation techniques, microtubers are more robust and easier to handle, which favors automatic planting processes (Dhital & Lim, 2012;Motallebi et al, 2013). Secondly, microtubers facilitate the maintenance and exchange of genetic material because small samples can be preserved, and subsequently sent in aseptic conditions (virus-free), even to countries with strong phytosanitary regulations (López, Sánchez, Mora, & Martínez, 2012).…”

Section: Introductionmentioning

confidence: 99%

Efecto del ácido bórico y la luz en el número y biomasa de microtubérculos de papa cv. “Floresta”

García-García¹,

Azofeifa-Bolaños

2017

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In order to understand the performance of Solanum tuberosum L. "Floresta" in producing microtubers, the effect of four concentrations of boric acid (6.2 mg l ) under two conditions-1) 8 hour dark and 2) 16 hour light photoperiod and 8 hour dark-was evaluated under in vitro culture conditions. Full-strength Murashige and Skoog medium was used as a control. The data analysis for the number and biomass of microtubers were significant for the condition (p ≤ 0.05). Concerning the concentration of boric acid and the interaction between factors, a significant difference was obtained for biomass (p ≤ 0.05). In dark conditions, a greater number of microtubers was obtained, although the biomass was lower compared to the light conditions where the biomass was higher, and the number of microtubers was lower. A concentration of 9.3 mg l -1 of boric acid in both types of conditions was the best treatment to produce a greater number and more biomass of microtubers. The differences identified in this work with regards to the number and biomass of microtubers were probably the combined result of genotype and specific culture conditions. Even considering this, the use of lighting conditions is proposed to induce larger and greener microtubers.

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Effect of sugar/osmotica levels on in vitro microtuberization of potato (Solanum tuberosum L.)

Cited by 10 publications

References 9 publications

Physiological Responses to High Exogenous Sucrose in Tuber Induction of Potato in Vitro

Physiological Responses to High Exogenous Sucrose in Tuber Induction of Potato in Vitro

Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha

Efecto del ácido bórico y la luz en el número y biomasa de microtubérculos de papa cv. “Floresta”

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