Effect of sucrose concentrations on in vitro growth and subsequent acclimatization of the native bromeliad Vriesea inflata (Wawra) Wawra

Freitas, Camila; Carvalho, Victória; Nievola, Catarina Carvalho

doi:10.5007/2175-7925.2015v28n3p37

Cited by 6 publications

(8 citation statements)

References 12 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…f.) Klotzsch & Otto e de 30 g.L -1 para Nidularium minutum Mez e Ananas comosus var. ananassoides (Baker) Coppens & F. Leal (Carvalho et al 2013;Freitas et al 2015;Andrade & Tamaki 2016;Santos et al 2017;Silva et al 2017). É descrito na literatura que altos níveis de sacarose no meio de cultura podem levar à um menor conteúdo de água disponível para os tecidos vegetais, resultando em alterações na emergência e no crescimento das plantas in vitro (George et al 2008;Bhatia et al 2015).…”

Section: Resultsunclassified

“…Plantas da bromélia Vriesea inflata cultivadas in vitro em diferentes concentrações de sacarose apresentaram um menor desenvolvimento com 30 g.L -1 ou mais deste açúcar, sendo que aquelas cultivadas em menores concentrações apresentaram o maior crescimento radicular in vitro e consequentemente originaram plantas maiores durante a etapa de aclimatização, que é a transferência de plantas cultivadas in vitro para condições ex vitro (Freitas et al 2015). Entretanto, o uso de 30 g.L -1 foi benéfico para a proliferação de calos de abacaxi (Ananas comosus (L.) Merr.…”

Section: Resultsunclassified

“…Tem sido demonstrado que o cultivo in vitro pode acelerar o tempo de germinação das sementes de A. imperialis, por isso é considerado um eficiente método de obtenção de plantas dessa espécie quando comparado ao cultivo convencional em sementeiras (Aoyama et al 2012), sendo que o satisfatório desenvolvimento das plantas pode estar associado ao fornecimento de sacarose (Freitas et al 2015), já que a morfogênese e o crescimento são processos que necessitam de energia (Buchanan et al 2015). Embora existam protocolos para a produção de A. imperialis (Kurita & Tamaki 2014), não foi relatada a influência da sacarose sobre o processo de obtenção de mudas a partir da germinação de sementes in vitro, coletadas de diferentes posições do escapo.…”

Section: Introductionunclassified

See 2 more Smart Citations

Armazenamento de sementes colhidas de diferentes posições do escapo floral para obtenção de plantas da bromélia imperial - Alcantarea imperialis

et al. 2020

Self Cite

View full text Add to dashboard Cite

Resumo A deiscência não simultânea dos frutos de bromélias deve ser considerada para fins de propagação por sementes. O objetivo deste estudo foi avaliar o melhor método para produção de plantas da bromélia imperial considerando a colheita simultânea das sementes de diferentes posições do escapo floral no período da deiscência dos frutos da base e seu armazenamento para posterior uso na obtenção de plantas. As sementes foram colhidas da base, meio e topo do escapo floral e armazenadas por 26, 61, 154 e 213 dias a 10 °C e a 25 °C. Foram avaliadas a emergência de plantas em gerbox com papel de filtro e a germinação e crescimento in vitro com 10, 20 e 30 g.L-1 de sacarose. As sementes colhidas da base e armazenadas por até dois meses a 10 °C foram as que apresentaram maior porcentagem de emergência de plantas (57%) e a melhor concentração de sacarose para germinação in vitro foi de 10 g.L-1 e para o crescimento, 20 g.L-1. Considerando-se a vulnerabilidade desta bromélia, o aproveitamento, ao mesmo tempo, das sementes de todo o escapo floral pode ser uma estratégia de conservação.

show abstract

Section: Resultsunclassified

Section: Introductionunclassified

See 1 more Smart Citation

Armazenamento de sementes colhidas de diferentes posições do escapo floral para obtenção de plantas da bromélia imperial - Alcantarea imperialis

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, survival percentage, number of shoots and vigorous growth with green plant of acclimatized pineapple plantlets under 3% table sugar and sucrose were validated as optimum dose when compared with glucose and fructose (Mengesha et al 2020). In native bromeliad, survival percentage of acclimatized plantlets under 1-6% sucrose was 100% with normal growth pattern, as indicated by number of leaves, number of roots, leaf length, root length, fresh mass and dry mass after transplanting to Pinus bark substrate for 90 day (Freitas et al 2015). In silk banana, survival rate in acclimatized plantlets under 3% glucose, fructose and sucrose was 100% with high growth performances, i.e., shoot length, shoot thickness and number of leaves, after transplanting for 45 day (secondary ex vitro hardening; Waman et al 2014).…”

Section: Discussionmentioning

confidence: 99%

In vitro acclimatization of <i>Curcuma longa</i> under controlled <i>iso</i>-osmotic conditions

Chintakovid

Tisarum

Samphumphuang

et al. 2021

Plant Biotechnology

View full text Add to dashboard Cite

In vitro acclimatization has been validated as the successful key to harden the plantlets before transplanting to ex vitro conditions. In the present study, we investigated the potential of different sugar types (glucose, fructose, galactose, sucrose) in regulating morphological, physiological and biochemical strategies, survival percentage and growth performance, and rhizome traits of turmeric under iso-osmotic potential. Leaf greenness (SPAD value) in acclimatized plantlets (4% glucose; −1.355 MPa osmotic potential) of 'ST018' was retained and greater than in 'PB009' by 1.69-fold, leading to maintain high F v /F m (maximum quantum yield of PSII), Φ PSII (photon yield of PSII) and P n (net photosynthetic rate) levels, and retained shoot height, leaf length, leaf width, shoot fresh weight and shoot dry weight after one month upon transplanting to ex vitro conditions. In addition, P n , C i (intracellular CO 2), g s (stomatal conductance) and E (transpiration rate) in acclimatized plantlets (6% sucrose; −1.355 MPa osmotic potential) of 'PB009' were stabilized as physiological adapted strategies, regulating the shoot and root growth and fresh and dry weights of mini-rhizome. Interestingly, the accumulation of total curcuminoids in mini-rhizome derived from 6% sucrose acclimatized plantlets of 'ST018' was greater than in 'PB009' by 3.76-fold. The study concludes that in vitro acclimation of turmeric 'PB009' and 'ST018' using 6% sucrose and 4% glucose, respectively, promoted percent survival, physiological adaptations, and overall growth performances under greenhouse conditions.

show abstract

“…Previously, survival percentage of carob tree (Cerafoma siliqua) was signi cantly improved by adding 58 mM glucose + 58 mM mannitol in the culture medium (Custódio et al 2004). Similarly, shoot and root traits of bromeliad plants (Vriesea in ata) derived from in-vitro sucrose containing medium were decreased (Freitas et al 2015), in relation to concentrations (4-6% Suc) and plant species (Wojtania et al 2019). In addition, root traits in the plantlets of magnolia cv.…”

Section: Ex-vitro Adaptationmentioning

confidence: 99%

In-Vitro Acclimatization of Curcuma Zedoaria Under Iso-Osmotic Treatments, Ex-Vitro Adaptation and Agronomic Traits in Greenhouse Conditions

Tisarum¹,

Bayaraa²,

Sotesaritkul³

et al. 2021

Preprint

View full text Add to dashboard Cite

Low survival rate, poor adaptation to ex-vitro environments, and time required for hardening the plants to cope with fluctuated environments of field trial are identified as major barriers in this technology. In present study, iso-osmotic adjustment in the culture medium using sucrose and/or mannitol was applied to the in-vitro cloning of Curcuma zedoaria (white turmeric) plants, which were transferred to ex-vitro conditions and subsequently cultivated in the greenhouse conditions prior to harvest after 9 months. During both in-vitro and ex-vitro development of plant, growth and physiological traits under 3% sucrose (Suc) + 2.5% mannitol (Man) were lower than those in control (3% Suc; conventional tissue culture). Interestingly, pseudostem height and root length in acclimatized plantlets under 3% Suc + 2.5% Man were sharply dropped by 60.13% and 92.37% over control, respectively, resulting in a decrease in the ex-vitro adaptation by 56.27% and 33.33% over the control. A positive relationship between reduction of net photosynthetic rate (Pn) and sucrose concentration in the leaf tissues was evidently observed. Remarkably, the morphological and physiological traits of aboveground and underground parameters of acclimatized plantlets under 3% Suc + 2.5% Man were maximized over control, leading to high yield of curcuminoids (229.4 mg plant− 1) in the dry rhizome (31 g plant− 1) when cultivated under greenhouse microenvironments for 9 months. Based on this investigation, we propose that plantlets of C. zedoaria micropropagated using 3% Suc + 2.5% Man can readily acclimatize under ex-vitro conditions and subsequently develop as healthy plants with compact and uniform size.

show abstract

Effect of sucrose concentrations on in vitro growth and subsequent acclimatization of the native bromeliad Vriesea inflata (Wawra) Wawra

Cited by 6 publications

References 12 publications

Armazenamento de sementes colhidas de diferentes posições do escapo floral para obtenção de plantas da bromélia imperial - Alcantarea imperialis

Armazenamento de sementes colhidas de diferentes posições do escapo floral para obtenção de plantas da bromélia imperial - Alcantarea imperialis

In vitro acclimatization of <i>Curcuma longa</i> under controlled <i>iso</i>-osmotic conditions

In-Vitro Acclimatization of Curcuma Zedoaria Under Iso-Osmotic Treatments, Ex-Vitro Adaptation and Agronomic Traits in Greenhouse Conditions

Contact Info

Product

Resources

About