Effect of sucrose concentration used for one-step dilution upon in vitro and in vivo survival of bovine embryos refrigerated in glycerol and 1, 2-propanediol

Viability of In‐vivo and In‐vitro Produced Bovine Embryos

“…Excellent to good quality frozenhhawed in-vivo produced embryos have a viability similar to fresh embryos (Lehn-Jensen, 1986;Callesen et al, 1992;Hasler, 1992;Leibo and Loskutoff, 1993 and many others). On the in-vitro produced embryos data are more scarce, but pregnancies have been established following transfer a limited number of embryos frozen in glycerol (Lu et al, 1988;Goto et al, 1989;Reichenbach et al, 1992b;Larsson et al, 1992;Kajihara et al, 1992;Zhang et al, 1992), in glycerollsucrose (Massip et al, 1993) and in 1,2 propandiol as cryoprotectant (Suzuki et al, 1990). Use of ethylene glycol as cryoprotectant seems to be slightly better than glycerol (Parks and Ruffing, 1992;Leibo and Loskutoff, 1993) and culture on buffalo rat liver cells for as little as 24 h prior to freezing improves the freezing resistance O/oelkel and .…”

Successful vitrification of bovine blastocysts, derived by in vitro maturation and fertilization

“…The results of the toxicity test showed that mere exposure to the vitrification solutions reduced embryonic survival with duration (Table 1) and that the toxicity of the solutions depended on the permeating component (Table 2). A low concentration (-12%) of propylene glycol has been reported to be effective for the freezing of bovine embryos; it diffuses out of the cell rapidly, and this allows direct transfer to recipients upon warming (Suzuki et al, 1990). At the very high concentration (40%) contained in the vitrification solution, however, this agent was found to be the most toxic of the three permeating cryoprotectants adopted here (Tables 1 , 2 ) .…”

Techniques for preservation of mammalian germ plasm

“…They are also very permeable to propylene glycol (PG) as well. Suzuki et al (1990) has utilized this fact to devise a very simple method to recover cryopreserved bovine embryos. Some of their results have been redrawn in Figure 3.…”

“…However, the same high percentage of embryos frozen in PG survived regardless of whether diluted with a sucrose solution or with isotonic saline alone. Furthermore, Suzuki et al produced The data are redrawn from observations of Suzuki et al, 1990. pregnancies in cattle by the direct transfer of cryopreserved embryos into recipients immediately after thawing, in a fashion analogous to artificial insemination.…”