Viability of In‐vivo and In‐vitro Produced Bovine Embryos

Greve, T.; Avery, B.; Callesen, H.

doi:10.1111/j.1439-0531.1993.tb00113.x

Cited by 73 publications

(38 citation statements)

References 55 publications

(37 reference statements)

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“…The differences between in vitro and in vivo produced embryos have been investigated by several authors (13,36). The composition of culture medium and particularly the presence of serum, has a marked influence on morphology and cytoplasmic appearance of in vitro-produced embryos that look dark.…”

Section: ~Troductionmentioning

confidence: 99%

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Díez

Heyman

Bourhis³

et al. 2001

Theriogenology

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To study the effect of partial removal of intracytoplasmatic lipids from bovine zygotes on their in vitro and in vivo survival, presumptive zygotes were delipidated by micromanipulation and cocultured with Veto cells in B2+10% FCS. Blastocyst rates of delipidated (n=960), sham (centrifuged but not delipidated, n=830) and control embryos (n=950) were 42.1, 42.3 and 39.9% respectively (P > 0.05). Day 7 blastocysts derived from delipidated zygotes had a mean of 123.9 + 45.6 nuclei compared to 137.5 + 32.9 for control blastocysts (P > 0.05). The full-term development of delipidated blastocysts after single transfer to recipients was similar to that of control IVF blastocysts (41.2% vs 45.4% respectively). To assess the effect of delipidation on the embryo tolerance to freczing/thawing, delipidated (n=73), control (n=67) and shmn (n=50) Day 7 blastocysts were frozen in 1.36 M glycerol + 0.25 M sucrose in PBS. After thawing, embryos were cocultured for 72 h with Vero cells in B2+10% FCS. Survival rates at 24 h were not significantly different between groups. However, in the delipidated group, the survival rate after 48 h in culture was significantly higher than in the control group (56.2 vs 39.8, P < 0.02), resulting in a higher hatching rate after 3 days in culture (45.2 vs 22.4, P < 0.02). Pregnancy rates for delipidated and control frozen/thawed embryos were respectively 10.5 and 22.2% (P > 0.05). Electron microscopic observations showed much fewer lipid droplets (and smaller) in delipated blastocysts than in controls. Taken together, our data show that delipidation of one cell stage bovine embryos is compatible with their normal development to term and has a beneficial effect on their tolerance to freezing and thawing at the blastocyst stage. This procedure, however, alters the developmental potential of such blastocysts, suggesting that maternally inherited lipid stores interfere with metabolic recovery after thawing.

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Section: ~Troductionmentioning

confidence: 99%

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Díez

Heyman

Bourhis³

et al. 2001

Theriogenology

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“…In any case, the quality of in vitroderived embryos and consequently their survival and viability after transfer or cryopreservation depend on the improvement of the in vitro methodology. Finally, the rate of early cleavage and freezing resistance could represent reliable criteria for assessment of the viability of such embryos (Greve et al, 1993).…”

Section: Statisticsmentioning

confidence: 99%

Survival and viability of fresh and frozen-thawed in vitro bovine blastocysts

Massip¹,

Mermillod²,

Langendonckt³

et al. 1995

Reprod. Nutr. Dev.

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“…The importance of ovary collection techniques and transfer expressions were discussed by diff~rent authors (6,7,10,11 Parameter Cultured ooeyte (n) Fertilized ooeyte (n) Fertilizationrate (%) Parthenogenesis(n) (%) PoJyspermia(n) (%) Chromatine degeneration Table 3. Fertilizationresults in study groups.…”

Section: Discussion and Conclusion Imentioning

confidence: 99%

“…(8) stated the every similarity of tecniques when making comparision. However the main demonstration of the maturation is fertilization (6,7). Since the fertilization rates of oocytes of large foIlides were significantly higher than of smaIler ones (49.3% vs 41.1 %).…”

Section: In Vitro Fertilizationmentioning

confidence: 99%

The effects of follicle diameter on the in vitro fertilization capacity of bovine oocytes aspirated from the slaugthered ovaries

Ün

Küplülü

2003

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of this study was to determine the maturation and fertilization capacities of bovine oocytes aspirated from slaugthered ovaries in relation with the follicle diameter. A number of 115 ovaries collected from the slaugthered cows at a local slaughterhouse were used as the materiaL. Peripheral follicles were counted according to the their diameters (2-6 mm-group i and 6-10 mm-group II). All follicles were punctured with an 18 G needIe hold on a 5 ml syringe and aspirated cumulus-oocyte complexes were classified in regard to their morphological apperance. Only Grade I, II and III oocytes were then placed in maturation medium (TCM-199+%20 (v/v) ECS+BSA) and incubated under an atmosphere of 5% C02 at 39°C for 22-24 hr. Af ter IVM, maturated oocytes were fertilized by adding 1-2 LLL (i X 10 6 ), swim-up separated sperm to the fertilization media (Tyrode's albumin lactate pyruvate medium-TALP) for in vitro fertilization under an atmosphere of 5% C02 at 39°C for 18-19 hr. Heparine (LO Ilg/ml) was used as the capacitating agent. The datas obtained at the all stages were recorded and statistical evaIuation was done with the Student's T test. A total of 549 (4.77:t2.09) oocytes were aspirated from 588 (5.11:t2.39) follicles with an aspiration rate of 93.3% in group i and 275 (2.5:t1.87) COC were aspirated from 300 (2.72:t1.58) follicles with an aspiration rate of 91.7% in group II. After the maturation period 401 of 549 oocytes in group I and 217 of 275 oocytes in group II were found as mature with amaturation rate of 73% and 78.9%, respectively (p>0.05). In 165 of 401 in group I (41.1 %) and 107 of 217 incubated oocytes in group II (49.3%), both male and female pronuclei were detected. As a conclusion, it was evident that the ovaries collected from the slaugtherhouse are suffİcient potentials for in vitro embryo production, although a great variation between the maturation and fertilization capacities of oocytes aspirated from the peripheral follicles could be observed. It was also obvious that there is a significiant relation between the follicle diameter and maturation and fertilization capacities of oocytes since the fertilization rates increases as the follicle diameter rises.Key words: Bovine, fertilization, in vitro, maturation, oocyteMezbahadan toplanan ovaryumlardan aspire edilen sığır oositlerinin in vitro fertilizasyonu üzerine follikül büyüklüklerinin etkisi Özet: Bu çalışmada, mezbahadan toplanan ovaryumlardan elde edilen sığır oositlerinin maturasyon ve fertilizasyon oranlarının folliklil çapı ile ilişkilendirilerek ortaya konması amaçlanmıştır. Çalışma materyali okdk bölge mezbahalarında kesilen hayvanlardan toplanan 115 ovaryum kullanıldı. Ovaryumların üzerlerindeki yüzeysel folliküller çaplarına göre (2-6 mm-Grup i ve 6-10 mm-Grup II) sayıldı. Tüm folliküllerin 18 G'lik iğne ile punksiyonları yapılarak oosider aspire edildi. Aspire edilen kumulus-oosit kompleksIeri morfolojilerine göre sınıflandırıldı. Yanlızca ı. ve II. kalite oosider in vitro maturasyon vasatına ECS+BSA) aktarılarak, 39°C...

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Viability of In‐vivo and In‐vitro Produced Bovine Embryos

Cited by 73 publications

References 55 publications

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Delipidating in vitro-produced bovine zygotes: Effect on further development and consequences for freezability

Survival and viability of fresh and frozen-thawed in vitro bovine blastocysts

The effects of follicle diameter on the in vitro fertilization capacity of bovine oocytes aspirated from the slaugthered ovaries

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