EFFECT OF SUBTILIN AND NISIN ON THE SPORES OF
            <i>BACILLUS COAGULANS</i>
            ,

Campbell, L. Leon; Sniff, Esther E.

doi:10.1128/jb.77.6.766-770.1959

Cited by 25 publications

(8 citation statements)

References 12 publications

(17 reference statements)

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“…Wynne et al (1952) Michener (1955) concluded that subtilin affects B. subtilis spores only in an environment suitable for germination. Campbell and Sniff (1959) demonstrated that neither subtilin nor nisin prevented spore germination or reduced heat resistance of spores of B. coagulans.…”

Section: Resultsmentioning

confidence: 97%

The Action of Tylosin on Spore‐Forming Bacteria

Greenberg

Silliker

1962

Journal of Food Science

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SUMMARY Tylosin at levels up to 100 ppm did not inhibit the germination of spores of Bacillus cereus and Clostridium species PA 3679 in brain heart infusion broth. Death of B. cereus in the presenee of Tylosin was correlated ehronologically with the start of exponential growth in Tylosin‐free control cultures. With PA 3679, the stage most susceptible to Tylosin occurred after germination but prior to exponential growth.

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Section: Resultsmentioning

confidence: 97%

The Action of Tylosin on Spore‐Forming Bacteria

Greenberg

Silliker

1962

Journal of Food Science

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“…Nisin inhibition of outgrowth must occur in the growth media, because nisin inhibits outgrowth rather than germination (Hitchins et al 1963;Could 1964). Outgrowth inhibition of spores activated in the presence of nisin but grown in its absence is caused by adsorbed rather than carried-over nisin (Campbell & Sniff 1959;Thorpe 1960). Therefore, salt must interfere with nisin adsorption, the first step in the lethal process (Hirsch 1954;Ramseier 1960;Thorpe 1960).…”

Section: Discussionmentioning

confidence: 99%

The effect of nisin‐sodium chloride interactions on the outgrowth of Bacillus licheniformis spores

Bell¹,

Lacy²

1985

Journal of Applied Bacteriology

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The effect of salt (NaCl) on the efficacy of nisin in preventing outgrowth of Bacillus licheniformis spores was determined in Plate Count Agar (PCA). An equivalent liquid medium was used for heat activation. Nisin and salt were added to the heat‐activation medium, the PCA, or both. The spores were extremely sensitive to nisin; outgrowth were completely inhibited in salt‐free media when 10 iu/ml of nisin was present in both the heat‐activation and the growth media or when 100 iu/ml nisin was present in either the heat‐activation and the growth medium. In media supplemented with 1% salt, outgrowth occurred from 1% of spores exposed to 100 iu/ml nisin in either the heat‐activation or the growth medium. A 3% salt supplement was necessary before detectable outgrowth occurred when both the heat‐activation and the growth media contained 100 iu/ml nisin. Salt appears to antagonize the sporicidal action of nisin by interfering with nisin adsorption onto the spore.

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“…Outgrowth, being dependent on biosynthesis of new cellular components, represents more metabolically sensitive sites for inhibition than do the rapidly degenerative changes of germination. For example, subtilin, nisin (Campbell & Sniff, 1959), chloramphenicol (Yoshikawa, 1965), actinomycin D, puromycin (Steinberg, Halvorson, Kenya & Weinberg, 1965) and chloroquin (McDonald, 1967) are all active at the outgrowth stage of development. The example examined in the present study, aminacrine hydrochloride, differed from chlorocresol not only in the stage against which it was active but also in retaining its inhibitory effects after treated spores had been repeatedly washed (Fig.…”

Section: Discussionmentioning

confidence: 99%

Some Effects of Preservatives on the Development of Bacterial Spores

Parker

1969

Journal of Applied Bacteriology

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The effects of preservatives on the development of spores of Bucillus subtilis have been examined using 2 indices of development sensitive to preservatives, namely, the swelling of spores prior to vegetative cell emergence an& O.D. changes of spore suspensions during germination and outgrowth. The preservatives used fall into two categories : (1) inhibitors of germination (chlorocresol and mixed esters of p-hydroxybenzoic acid), and (2) inhibitors of postgerminative development (cetrimide, aminacrine and phenylmercuric nitrate). None of the preservatives prevented the initial phase of spore swelling and it is suggested that this is attributable t o a nonmetabolic (inanimate) hydration. The inhibition of germination by a category 1 preservative (chlorocresol) is readily reversible by washing and to prevent germination the preservative must be added early in the process. The inhibition of postgerminative development by a, category 2 preservative (aminacrine) cannot be reversed by washing treated spores.WHEN CHEMICAL AGENTS are used to prevent the development of bacterial spores to vegetative cells they exert their effect at different stages in the transition. The stage in development of the spore at which particular preservatives are effective has been found by microscopic examinat)ion of spore preservative systems (Gould, 1964). Alternative methods of obtaining this information are described here. In these, 2 indices of spore development which are sensitive to preservatives are studied : (1) the swelling during germination and (2) the optical density (o.D.) changes which accompany germination and outgrowth.

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EFFECT OF SUBTILIN AND NISIN ON THE SPORES OF BACILLUS COAGULANS ,

Cited by 25 publications

References 12 publications

The Action of Tylosin on Spore‐Forming Bacteria

The Action of Tylosin on Spore‐Forming Bacteria

The effect of nisin‐sodium chloride interactions on the outgrowth of Bacillus licheniformis spores

Some Effects of Preservatives on the Development of Bacterial Spores

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