Effect of sub-lethal doses of Bacillus thuringiensis subsp. Aizawai and deltamethrin with regard to fertility and organ toxicity in pregnant albino rats

Lemos-Jordão, Ana Janaina Jeanine Martins de; Siqueira, Herbert A.A.; Wanderley‐Teixeira, Valéria; Maia, Frederico Celso Lyra; Teixeira, Álvaro Aguiar Coelho; Silva, Edson J.; Oliveira, José Vargas de

doi:10.1016/j.etp.2012.02.004

Cited by 16 publications

(17 citation statements)

References 39 publications

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“…However, no clinical signs of intoxication were reported, and neonates did not exhibit signs of malformations of the head, limbs, thorax or abdomen (Lemos et al, 2013). Additionally, studies that are more complete are required to indicate precisely whether the abnormalities detected were presented in all rats or what percentage of animals were affected because the above-mentioned studies only presented representative tissue samples and because the data presented suggested that sublethal doses administered can provide chronic toxicity in humans (Lemos et al, 2013). Indeed, pesticide formulations have been demonstrated to contain toxic adjuvants that are sometimes more toxic than their active principles and that could be responsible for secondary toxic effects (Mesnage et al, 2013a).…”

Section: Action Mechanism Of Cry Toxins In Insectsmentioning

confidence: 99%

“…For example, Lemos et al (2013) tested three sub-lethal doses of the biological insecticide XenTari W WG (B. thuringiensis subsp. However, no clinical signs of intoxication were reported, and neonates did not exhibit signs of malformations of the head, limbs, thorax or abdomen (Lemos et al, 2013). However, no clinical signs of intoxication were reported, and neonates did not exhibit signs of malformations of the head, limbs, thorax or abdomen (Lemos et al, 2013).…”

Section: Action Mechanism Of Cry Toxins In Insectsmentioning

confidence: 99%

“…(Lemos et al, 2013) B. thuringiensis kurstaki and B. thuringiensis israelensis Seven human cell lines were exposed Bti/Btk products generated nonspecific cytotoxicities involving loss of bioreduction, and rounding, blebbing and detachment of cells. (Lemos et al, 2013) B. thuringiensis kurstaki and B. thuringiensis israelensis Seven human cell lines were exposed Bti/Btk products generated nonspecific cytotoxicities involving loss of bioreduction, and rounding, blebbing and detachment of cells.…”

Section: B Thuringiensis Serovar Konkukianmentioning

confidence: 99%

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An overview of the safety and biological effects of Bacillus thuringiensis Cry toxins in mammals

Rubio-Infante

Moreno‐Fierros

2015

J of Applied Toxicology

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Crystal proteins (Cry) produced during the growth and sporulation phases of Bacillus thuringiensis (Bt) bacterium are known as delta endotoxins. These toxins are being used worldwide as bioinsecticides to control pests in agriculture, and some Cry toxins are used against mosquitoes to control vector transmission. This review summarizes the relevant information currently available regarding the biosafety and biological effects that Bt and its insecticidal Cry proteins elicit in mammals. This work was performed because of concerns regarding the possible health impact of Cry toxins on vertebrates, particularly because Bt toxins might be associated with immune-activating or allergic responses. The controversial data published to date are discussed in this review considering earlier toxicological studies of B. thuringiensis, spores, toxins and Bt crops. We discussed the experimental studies performed in humans, mice, rats and sheep as well as in diverse mammalian cell lines. Although the term 'toxic' is not appropriate for defining the effects these toxins have on mammals, they cannot be considered innocuous, as they have some physiological effects that may become pathological; thus, trials that are more comprehensive are necessary to determine their effects on mammals because knowledge in this field remains limited.

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Section: Action Mechanism Of Cry Toxins In Insectsmentioning

confidence: 99%

Section: Action Mechanism Of Cry Toxins In Insectsmentioning

confidence: 99%

Section: B Thuringiensis Serovar Konkukianmentioning

confidence: 99%

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An overview of the safety and biological effects of Bacillus thuringiensis Cry toxins in mammals

Rubio-Infante

Moreno‐Fierros

2015

J of Applied Toxicology

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“…subsp. aizawai reduced fertility and caused lesions in the kidneys, liver and lungs in rats (Lemos et al, 2013).…”

Section: Minireview 3 Evaluating Genetic Stability Of Microorganismsmentioning

confidence: 99%

“…Several reports were on infections caused by Aureobasidium pullulans, which includes PPP strains, (e.g. Joshi et al, 2009;Mise et al, 2008;Oliveira et al, 2013), in addition to one study on Aureobasidium proteae (Kutleša et al, 2012). Other case reports were on infections associated with Beauveria bassiana (Gürcan et al, 2006;Oh et al, 2009;Tucker et al, 2004), Metarhizium anisopliae (Chung et al, 2005;Motley et al, 2011;Osorio et al, 2007), Paraconiothyrium cyclothyrioides (Gordon et al, 2012), Pseudozyma antarctica (Sugita et al, 2003), and Pseudozyma aphidis (e.g.…”

Section: Pathogenicity Traits Of Selected Microorganisms Within the Smentioning

confidence: 99%

Literature search and data collection on RA for human health for microorganisms used as plant protection products

Hackl

Pacher‐Zavisin

Sedman

et al. 2015

EFSA Supporting Publications

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A knowledge base of scientific and regulatory data relevant in the context of risk assessment of microbial plant protection products (PPPs) for human health was established, taking into consideration conditions 1 to 4 in Regulation (EU) 546/2011 that must be met before authorisation can be granted. We screened scientific publications and documents from governmental and institutional sources and extracted relevant information into a database, performed comparative genome analyses to explore possibilities and limitations of applying read-across among PPP strains, and discussed the collective findings among a group of experts in relevant fields. Besides information on factors relevant for the evaluation of microbial PPP strains regarding pathogenicity, infectivity, and toxicity, information was collected on methods used to analyse the PPP species' ability to persist and grow in test animals, for detecting microbial DNA transfer, for testing microbial compounds for potential toxicity, and for detecting toxin production from PPP strains. Questions arising from the reviewed scientific and grey literature were how to address the specific biological properties of PPP strains and how to take into consideration realistic exposure scenarios and a natural background of environmental microorganisms in the risk assessment. Results from the present work imply that pathogenicity traits have to be considered as strain specific and that, hence, read-across comparison cannot be used. From a scientific point of view, it was highly recommended to provide whole genome sequencing data of PPP strains to allow their unambiguous identification, while other technological developments such as the use of metabolomics data were seen as an option for future implementation. It is envisaged to further develop the key topics of the present work at a joint stakeholder workshop as the next step towards the preparation of a guidance document by the PPR panel on how to conduct the regulatory risk assessment for microbial pesticides. © Efsa 2015Key words: microorganism, pesticide, risk assessment, human health, toxicity, read-across, DNA sequencing Disclaimer: The present document has been produced and adopted by the bodies identified above as authors. This task has been carried out exclusively by the authors in the context of a contract between the European Food Safety Authority and the authors, awarded following a tender procedure. The present document is published complying with the transparency principle to which the Authority is subject. It may not be considered as an output adopted by the Authority. The European Food Safety Authority reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors.Suggested citation: Evelyn Hackl1), Margit Pacher-Zavisin2), Laura Sedman1), Stefan Arthaber1), Ulla Bernkopf1), Günter Brader1), Markus Gorfer1), Birgit Mitter1), Aspasia Mitropoulou1), Monika Schmoll1), Willem van Hoesel1), Elisabeth Wis...

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Deltamethrin‐induced oxidative stress and mitochondrial caspase‐dependent signaling pathways in murine splenocytes

Kumar

Sasmal

Bhaskar

et al. 2014

Environmental Toxicology

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Deltamethrin (DLM) is a well-known pyrethroid insecticide used extensively in pest control. Exposure to DLM has been demonstrated to cause apoptosis in various cells. However, the immunotoxic effects of DLM on mammalian system and its mechanism is still an open question to be explored. To explore these effects, this study has been designed to first observe the interactions of DLM to immune cell receptors and its effects on the immune system. The docking score revealed that DLM has strong binding affinity toward the CD45 and CD28 receptors. In vitro study revealed that DLM induces apoptosis in murine splenocytes in a concentration-dependent manner. The earliest markers of apoptosis such as enhanced reactive oxygen species and caspase 3 activation are evident as early as 1 h by 25 and 50 µM DLM. Western blot analysis demonstrated that p38 MAP kinase and Bax expression is increased in a concentration-dependent manner, whereas Bcl 2 expression is significantly reduced after 3 h of DLM treatment. Glutathione depletion has been also observed at 3 and 6 h by 25 and 50 µM concentration of DLM. Flow cytometry results imply that the fraction of hypodiploid cells has gradually increased with all the concentrations of DLM at 18 h. N-acetyl cysteine effectively reduces the percentage of apoptotic cells, which is increased by DLM. In contrast, buthionine sulfoxamine causes an elevation in the percentage of apoptotic cells. Phenotyping data imply the effect of DLM toxicity in murine splenocytes. In brief, the study demonstrates that DLM causes apoptosis through its interaction with CD45 and CD28 receptors, leading to oxidative stress and activation of the mitochondrial caspase-dependent pathways which ultimately affects the immune functions. This study provides mechanistic information by which DLM causes toxicity in murine splenocytes. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 808-819, 2016.

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Effect of sub-lethal doses of Bacillus thuringiensis subsp. Aizawai and deltamethrin with regard to fertility and organ toxicity in pregnant albino rats

Cited by 16 publications

References 39 publications

An overview of the safety and biological effects of Bacillus thuringiensis Cry toxins in mammals

An overview of the safety and biological effects of Bacillus thuringiensis Cry toxins in mammals

Literature search and data collection on RA for human health for microorganisms used as plant protection products

Deltamethrin‐induced oxidative stress and mitochondrial caspase‐dependent signaling pathways in murine splenocytes

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