Effect of Streptozotocin Diabetes and Insulin
Administration on Some Liver Enzyme Activities in the
Post-Weaning Rat

Storey, Janet M.; Bailey, E.

doi:10.1159/000458606

Cited by 32 publications

(15 citation statements)

References 17 publications

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“…The glycogenetic enzyme that must adapt in the diabetic condition to yield energy was less depended upon when these dietary supple ments were fed to rats (table 7). This meta bolic shift caused by dietary fibre supplements is similar to those reported for streptozotocin-induced diabetic rats later in jected with insulin [16]. Along with this shift is the observation of less dependence on gly cogen by diabetic rats fed the test fibres.…”

Section: Discussionsupporting

confidence: 84%

See 1 more Smart Citation

Digestive and Hepatic Enzymes in Streptozotocin-lnduced Diabetic Rats Fed Supplements of Dikanut <i>(Irvingia gabonensis)</i>and Cellulose

Omoruyi

Adamson²

1993

Ann Nutr Metab

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In a feeding trial involving dikanut (an African viscous fibre) and cellulose (a particulate fibre), diabetic rats were maintained for 4 weeks on each of the test fibres. The levels of digestive and membrane-bound enzymes of the intestine, and hepatic glycolytic enzymes were determined. The effect of the supplements on intestinal morphology was also assessed. The two types of dietary fibre caused a general reduction in the levels of all the intestinal enzymes assayed with the effect of dikanut supplementation resulting in more drastic reductions. Evidence was obtained for a marked alteration in the intestinal morphology. It was concluded that the reduced absorption of glucose resulted in its lowered level in the blood and urine. The disruption of the mucosal membrane may also curtail the absorption of glucose. On the other hand, the activities of hepatic glycolytic enzymes became elevated to efficiently utilize the low substrates reaching the liver. The dietary fibres caused a shift away from the depletion of glycogen by the diabetic rat to synthesis of the storage polysaccharide. The long-term effects of the several adaptive responses resulting in lowered blood glucose from feeding these dietary fibre supplements to the diabetic rat requires further research.

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Section: Discussionsupporting

confidence: 84%

“…The activ ¡ties of phosphofructoki nase. pyruvate kinase and NADP-isocitratc dehydrogenase in the liver were de termined by measuring the change in extinction due to NADP reduction or NADH oxidation [16]. Glucosc- …”

Section: Methodsmentioning

confidence: 99%

Digestive and Hepatic Enzymes in Streptozotocin-lnduced Diabetic Rats Fed Supplements of Dikanut <i>(Irvingia gabonensis)</i>and Cellulose

Omoruyi

Adamson²

1993

Ann Nutr Metab

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“…Two enzymes which reg ulate hepatic glycolysis are 6-phosphofructokinase 1 (PFK1; EC 2.7.1.11) and pyruvate kinase (PK; EC 2.7.1.40) [5]. The activities of these two enzymes can be regulated coordi nate^: for example, the activities of both arc reduced in diabetes and by glucagon, but are stimulated by insulin [6], In adult liver, both enzymes are under allosteric control [7][8][9], In smaller mammals (e.g. rats), fetal liver mitochondria are relatively undeveloped, and there is a high rate of glycolysis, which is accompanied by high activities of PFK1 and PK [10].…”

Section: Introductionmentioning

confidence: 99%

Development of Enzymes of Glycolysis and Gluconeogenesis in Human Fetal Liver

Sadava

Frykman²,

Harris³

et al. 1992

Neonatology

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The activities of two key enzymes of glycolysis and two key enzymes of gluconeogenesis were measured in liver samples from 44 human fetuses ranging in gestational age from 20 weeks to term, from infants to 10 years and from adults from 21 to 58 years. Specific activities of both gluconeogenic enzymes, fructose1,6-biphosphatase and phosphoenolpyruvate carboxykinase, increased throughout the period of fetal development examined, and rose to near adult levels after birth. The activities of both glycolytic enzymes, phosphofructokinase 1 and pyruvate kinase, were lower in fetal than in pediatric and adult samples. For both of these enzymes, there was a significant reduction in activity of livers from fetuses of 34–37 weeks’ gestation. Both enzymes showed hyperbolic kinetics at 24 weeks’ gestation, but this changed to sigmoid kinetics during the 34-37 weeks’ period of low activity. The data indicate that during the last weeks of gestation, inhibition of the activities of these two glycolytic enzymes, coupled with the rise in the two gluconeogenic enzymes, may reflect a change in liver from a primarily glycolytic role in the first two trimesters to a gluconeogenic role shortly before birth.

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“…The intestine was inverted and the mu cosa was removed by scraping with a glass slide. Known weights of the colonic tissue samples and the liver samples were then homogenized in appropriate buffers [15][16][17][18], centrifuged at 3,000 £ and the super natant frozen until needed for the enzyme assays. Phosphofructokinasc, pyruvate kinase, malic en zyme.…”

Section: Methodsmentioning

confidence: 99%

“…Phosphofructokinasc, pyruvate kinase, malic en zyme. isocitrate dehydrogenase, glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and ATP-citrate lyase were estimated by measuring the oxidation or reduction of NAD(P)(H) [15]. Glu cose 6-phosphate dehydrogenase was determined by measuring the amount of inorganic phosphate liberat ed from glucose 6-phosphatc [16][17][18].…”

Section: Methodsmentioning

confidence: 99%

Early Changes in Energy Metabolism in Rats Exposed to an Acute Level of Deoxycholate and Fed a Nigerian-Like Diet

Eriyamremu

Adamson

1994

Ann Nutr Metab

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Early alterations in cellular energy metabolism, reductive biosynthesis and enzymes related to cell proliferation were studied in 40 Wistar albino rats exposed to an acute level of deoxycholate (DOC), and fed different diets. The animals were divided into four equal groups and fed ad libitum either a normal diet (ND), a high-carbohydrate high-fibre (HCF) diet, or a high-protein high-fat (HPF) diet. Three times weekly intrarectal injection of 40 mg/0.2 ml DOC was given to three groups of the rats for 9 weeks. The specific activities of phosphofructokinase, pyruvate kinase, lactate dehydrogenase, glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were all significantly (p < 0.05) increased in the DOC-treated animals compared with the physiological saline-treated control. Reductive biosynthetic enzyme activities (malic enzyme, isocitrate dehydrogenase-NADP⁺-dependent, and ATP-citrate lyase) were reduced in the DOC-treated animals compared with the control. Feeding rats with the HCF diet significantly (p < 0.05) lowered the specific activities of the enzymes of glycolysis, of the pentose phosphate pathway (oxidative) and hyaluronidase and proteinase compared with those of the HPF-fed rats. These results show an altered enzymic profile in rats fed an HCF and an HPF diet compared with rats fed the ND and suggests a protective role of the HCF diet against the development of colon cancer.

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Effect of Streptozotocin Diabetes and Insulin Administration on Some Liver Enzyme Activities in the Post-Weaning Rat

Cited by 32 publications

References 17 publications

Digestive and Hepatic Enzymes in Streptozotocin-lnduced Diabetic Rats Fed Supplements of Dikanut <i>(Irvingia gabonensis)</i>and Cellulose

Digestive and Hepatic Enzymes in Streptozotocin-lnduced Diabetic Rats Fed Supplements of Dikanut <i>(Irvingia gabonensis)</i>and Cellulose

Development of Enzymes of Glycolysis and Gluconeogenesis in Human Fetal Liver

Early Changes in Energy Metabolism in Rats Exposed to an Acute Level of Deoxycholate and Fed a Nigerian-Like Diet

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