Effect of sterilisation by gamma irradiation on the ability of polycaprolactone (PCL) to act as a scaffold material

Cottam, Emily; Hukins, D.W.L.; Lee, Kenneth; Hewitt, Christopher J.; Jenkins, Michael J.

doi:10.1016/j.medengphy.2008.07.005

Cited by 88 publications

(80 citation statements)

References 17 publications

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“…This was in agreement with the previous reports [25]. In addition to this, our study had shown that while increasing the irradiation dose from 15 kGy to 65 kGy, the cell proliferation also increased.…”

Section: Fibroblast Cell Proliferationsupporting

confidence: 94%

Dose-Dependent Effects of Gamma Irradiation on the Materials Properties and Cell Proliferation of Electrospun Polycaprolactone Tissue Engineering Scaffolds

Augustine

Saha

Jayachandran

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

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Electrospun membranes of polycaprolactone are widely used for biomedical applications like wound dressings and tissue engineering scaffolds. It is important to sterilize this material using the most accepted method, the gamma irradiation. In this study, we have evaluated the sterilizability of electrospun polycaprolactone membranes with gamma radiation of varying doses. The irradiated materials were assessed for the changes in morphology, crystallinity, surface degradation, hydrophilicity, mechanical property, sterility and the cell proliferation. Our results demonstrate that electrospun polycaprolactone can be effectively sterilized by gamma irradiation, however a higher dose of radiation affect the 2 materials properties. The irradiated membranes showed improved hydrophilicity and fibroblast cell proliferation.

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Section: Fibroblast Cell Proliferationsupporting

confidence: 94%

Dose-Dependent Effects of Gamma Irradiation on the Materials Properties and Cell Proliferation of Electrospun Polycaprolactone Tissue Engineering Scaffolds

Augustine

Saha

Jayachandran

et al. 2015

International Journal of Polymeric Materials and Polymeric Biom

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show abstract

“…Nanofibrous implants have highly porous structure and contain far less material than materials made of solid poly(lactic acid). There is a family of polyesters – aliphatic polycarbonates that generate only very weak carbonic acid and therefore they have limited impact on the tissue irritation [16]–[18]. Polyesters are created de novo , no animal material has to be used to synthesize them.…”

Section: Introductionmentioning

confidence: 99%

Ureter Regeneration–The Proper Scaffold Has to Be Defined

et al. 2014

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The aim of this study was to compare two different acellular scaffolds: natural and synthetic, for urinary conduit construction and ureter segment reconstruction. Acellular aortic arch (AAM) and poly(L-lactide-co-caprolactone) (PLCL) were used in 24 rats for ureter reconstruction in both tested groups. Follow-up period was 4 weeks. Intravenous pyelography, histological and immunohistochemical analysis were performed. All animals survived surgical procedures. Patent uretero-conduit junction was observed only in one case using PLCL. In case of ureter segment reconstruction ureters were patent in one case using AAM and in four cases using PLCL scaffolds. Regeneration of urothelium layer and focal regeneration of smooth muscle layer was observed on both tested scaffolds. Obtained results indicates that synthetic acellular PLCL scaffolds showed better properties for ureter reconstruction than naturally derived acellular aortic arch.

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“…However, no study on ethanol as sterilize agent has been performed on protein-based biomaterials. Gamma irradiation is a common technique for sterilizing polymeric implants (20). Since scaffolds usually have a porous structure, a sterilization method is required that can penetrate such materials without leaving residues.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Sterilization Methods on the Physical Properties of Silk Sericin Scaffolds

2011

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Abstract. Protein-based biomaterials respond differently to sterilization methods. Since protein is a complex structure, heat, or irradiation may result in the loss of its physical or biological properties. Recent investigations have shown that sericin, a degumming silk protein, can be successfully formed into a 3-D scaffolds after mixing with other polymers which can be applied in skin tissue engineering. The objective of this study was to investigate the effectiveness of ethanol, ethylene oxide (EtO) and gamma irradiation on the sterilization of sericin scaffolds. The influence of these sterilization methods on the physical properties such as pore size, scaffold dimensions, swelling and mechanical properties, as well as the amount of sericin released from sericin/polyvinyl alcohol/glycerin scaffolds, were also investigated. Ethanol treatment was ineffective for sericin scaffold sterilization whereas gamma irradiation was the most effective technique for scaffold sterilization. Moreover, ethanol also caused significant changes in pore size resulting from shrinkage of the scaffold. Gamma-irradiated samples exhibited the highest swelling property, but they also lost the greatest amount of weight after immersion for 24 h compared with scaffolds obtained from other sterilization methods. The results of the maximum stress test and Young's modulus showed that gamma-irradiated and ethanol-treated scaffolds are more flexible than the EtO-treated and untreated scaffolds. The amount of sericin released, which was related to its collagen promoting effect, was highest from the gamma-irradiated scaffold. The results of this study indicate that gamma irradiation should have the greatest potential for sterilizing sericin scaffolds for skin tissue engineering.

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Effect of sterilisation by gamma irradiation on the ability of polycaprolactone (PCL) to act as a scaffold material

Cited by 88 publications

References 17 publications

Dose-Dependent Effects of Gamma Irradiation on the Materials Properties and Cell Proliferation of Electrospun Polycaprolactone Tissue Engineering Scaffolds

Dose-Dependent Effects of Gamma Irradiation on the Materials Properties and Cell Proliferation of Electrospun Polycaprolactone Tissue Engineering Scaffolds

Ureter Regeneration–The Proper Scaffold Has to Be Defined

The Effect of Sterilization Methods on the Physical Properties of Silk Sericin Scaffolds

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