Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathway in vitro

Zhou, Qiang; Zhang, Ge; Long, Haibo; Lei, Fei; Ye, Fei; Jia, Xufeng; Zhou, Yunlong; Kang, Jianping; Feng, Daxiong

doi:10.1016/s1995-7645(14)60094-8

Cited by 14 publications

(16 citation statements)

References 25 publications

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“…The Notch signal pathway renders the differentiation of spinal cord-derived stem cells into nerve cells, and inhibits that of neural stem cells into neurogliocytes, which contributes to the therapy for SCI (22). Notch signaling serves an important role in the development of the central nervous system, and Notch is essential for the directional differentiation and maintenance of the neural precursor cells (23). In a recent study, it was found that Notch and its relevant proteins were persistently expressed in the brain hippocampal area of adult mammals (24).…”

Section: Discussionmentioning

confidence: 99%

“…It has been identified that manual intervention may promote the regeneration of nerve cells, and that moderate trauma can promote the proliferation and differentiation of the neural precursor cells that are in the stationary phase into nerve cells in the hippocampus of the mouse brain (33). In adult mice, the cells that are in the stationary phase (such as somatic stem cells) still have the ability to restart proliferation following years of cell cycle arrest; however, this requires changes in the expression quantity of the Hes1 protein, suggesting that Hes1 protein serves an important role in the development of the somatic cells, particularly the somatic stem cells (23). In addition, microglia activation, astrocyte proliferation and glial scar formation subsequent to SCI are the major factors that hinder the effective regeneration and growth of the injured axons (34).…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA‑381/Hes1 is a potential therapeutic target for spinal cord injury

et al. 2018

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The aim of the present study was to investigate whether microRNA‑381 is a potential therapeutic target for spinal cord injury (SCI) and its possible mechanism. Reverse transcription quantitative polymerase chain reaction (qPCR) for mRNA expression was used to analyze the changes of microRNA-381 expression. Cell viability and cell apoptosis were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and ﬂow cytometry. Caspase‑3 activity was measured using caspase‑3 activity kit, and western blot analysis was used to measure the protein expression of neurogenic locus notch homolog protein 1 (Notch1), notch 1 intracellular domain (NICD) and transcription factor HES-1 (Hes1). The data showed that microRNA‑381 expression of model SCI rats was downregulated compared with that of control rats. Overexpression of microRNA‑381 promoted cell proliferation, and inhibited apoptosis and caspase‑3 and apoptosis regulator BAX (Bax) protein expression in neurocytes. Overexpression of microRNA‑381 also increased Wnt and β‑catenin protein expression, and suppressed the protein expression of Notch1, NICD and Hes1 in neurocytes. Wnt inhibitor, Wnt‑C59 (1 µmol/l), inhibited cell proliferation, promoted apoptosis and caspase‑3 and Bax protein expression, suppressed β‑catenin protein expression and induced Hes1 protein expression in neurocytes following microRNA‑381 overexpression. Notch inhibitor, FLI‑06 (1 µmol/l), promoted cell proliferation, inhibited apoptosis and caspase‑3 and Bax protein expression, and suppressed NICD and Hes1 protein expression in neurocytes following microRNA‑381 overexpression. Thus, this study showed that overexpression of microRNA‑381 promotes cell proliferation of neurocytes in SCI via Hes1 expression, which may be a novel important mechanism for SCI in clinical applications.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MicroRNA‑381/Hes1 is a potential therapeutic target for spinal cord injury

et al. 2018

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“…Increased expression of Pax6 positively promote NSC self-renewal and neurogenesis ( Osumi et al, 2008 ; Sansom et al, 2009 ; Curto et al, 2014 ; Gan et al, 2014 ). The Notch1 signaling pathway has also been demonstrated to control NSC fate ( Kiparissides et al, 2011 ; Zhou et al, 2014 ; Stergiopoulos and Politis, 2016 ). The heatmap results indicated that the expression of several key stemness-related genes was upregulated in the Space group.…”

Section: Resultsmentioning

confidence: 99%

Systematic Analysis of mRNA and miRNA Expression of 3D-Cultured Neural Stem Cells (NSCs) in Spaceflight

Cui

Han

Xiao

et al. 2018

Front. Cell. Neurosci.

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Recently, with the development of the space program there are growing concerns about the influence of spaceflight on tissue engineering. The purpose of this study was thus to determine the variations of neural stem cells (NSCs) during spaceflight. RNA-Sequencing (RNA-Seq) based transcriptomic profiling of NSCs identified many differentially expressed mRNAs and miRNAs between space and earth groups. Subsequently, those genes with differential expression were subjected to bioinformatic evaluation using gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) and miRNA-mRNA network analyses. The results showed that NSCs maintain greater stemness ability during spaceflight although the growth rate of NSCs was slowed down. Furthermore, the results indicated that NSCs tended to differentiate into neuron in outer space conditions. Detailed genomic analyses of NSCs during spaceflight will help us to elucidate the molecular mechanisms behind their differentiation and proliferation when they are in outer space.

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“…The NSCs in the dentate gyrus (DG) differentiated into neurons largely, which improved the spatial learning and memory ability of the mice and further restored the neurological function [ 114 ] (seen in Figure 2 ). The extracts from the injured spinal cord upregulated Notch1 mRNA, and the expression of Hes1 subsequently activated the Notch signaling pathway to promote NSC proliferation [ 115 ] (seen in Figure 2 ). Nevertheless, silencing the expression of Notch1 inhibited the division of NSCs and prevented NSCs from entering the cell cycle and maintaining self-renewal.…”

Section: Cell Signaling Pathwaysmentioning

confidence: 99%

The Effects of Different Factors on the Behavior of Neural Stem Cells

Huang

Wang

2017

Stem Cells International

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The repair of central nervous system (CNS) injury has been a worldwide problem in the biomedical field. How to reduce the damage to the CNS and promote the reconstruction of the damaged nervous system structure and function recovery has always been the concern of nerve tissue engineering. Multiple differentiation potentials of neural stem cell (NSC) determine the application value for the repair of the CNS injury. Thus, how to regulate the behavior of NSCs becomes the key to treating the CNS injury. So far, a large number of researchers have devoted themselves to searching for a better way to regulate the behavior of NSCs. This paper summarizes the effects of different factors on the behavior of NSCs in the past 10 years, especially on the proliferation and differentiation of NSCs. The final purpose of this review is to provide a more detailed theoretical basis for the clinical repair of the CNS injury by nerve tissue engineering.

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Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathway in vitro

Cited by 14 publications

References 25 publications

MicroRNA‑381/Hes1 is a potential therapeutic target for spinal cord injury

MicroRNA‑381/Hes1 is a potential therapeutic target for spinal cord injury

Systematic Analysis of mRNA and miRNA Expression of 3D-Cultured Neural Stem Cells (NSCs) in Spaceflight

The Effects of Different Factors on the Behavior of Neural Stem Cells

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