Effect of Some Botanicals on Hemocytes and Molting of Papilio demoleus Larvae

Pandey, Suyog; Pandey, Jay Prakash; Tiwari, Rajesh

doi:10.3923/je.2012.23.31

Cited by 17 publications

(16 citation statements)

References 13 publications

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“…Toxic substances, such as pesticides and heavy metals, decrease the number of hemocytes 19,53 . Studies have reported that toxic substances can alter the immunological response 54 , inhibit hematopoietic function 55 , decrease mitotic activity 56 , and induce apoptosis 57 . Kim et al 3 .…”

Section: Discussionmentioning

confidence: 99%

Effects of Selenium and Vitamin E on Enzymatic, Biochemical, and Immunological Biomarkers in Galleria mellonella L

Coşkun

Kayış

Gulsu

et al. 2020

Sci Rep

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To understand the effects of micronutrients have particular biological functions that are involved mainly in the antioxidant system, which has essential implications for the development of diseases, this study investigated how vitamin E, selenium, and their combination affect lipid, protein, carbohydrate, and malondialdehyde (MDA) content; antioxidant enzyme (catalase [CAT], superoxide dismutase [SOD], glutathione-S-transferase [GST]) activity; and the total hemocyte count (THC) in larvae of Galleria mellonella L. fed different diets. Diet 1 (100 µg of selenium) significantly decreased carbohydrate and lipid content. Diets 2 (100 µg of vitamin E), 3 (100 µg of selenium and vitamin E each), and 5 (Tween 80) did not significantly affect protein and carbohydrate content. Diet 2 significantly increased the lipid content compared to diet 4 (control). Diet 1 increased CAT, SOD, and GST activity and MDA content (highest at 27.64 nmol/mg protein). Diet 2 significantly decreased SOD activity and MDA content compared to other diets. Diet 1 significantly decreased the THC compared to other diets. These results suggested that selenium changes oxidative stress parameters, energy reserves, and THC in G. mellonella. These changes could be a physiological adaptation against selenium-induced oxidative stress. Vitamin E could play a protective role in selenium toxicity. Trace elements play a significant role in many biochemical and physiological processes and therefore are essential for living organisms 1. Selenium (symbol Se), a trace element that is a major component of glutathione peroxidase, plays an important role in intracellular defense against reactive oxygen species (ROS), in addition to being essential for growth and development 2. However, high selenium levels can be toxic 3,4. The utility and toxicity of selenium depend on its chemical form and concentration; in its inorganic form (selenates, selenides, and selenite), selenium is more toxic and less useful compared to its organic forms (selenomethionine and selenocysteine). Excessive selenium levels have cytotoxic, genotoxic, and carcinogenetic effects due to ROS accumulation and increased oxidative stress 4,5. Cells use antioxidants to neutralize the harmful effects of ROS. Many of these antioxidants decrease DNA damage, decrease protein denaturation or loss, and prevent lipid peroxidation 6,7. In insects, ascorbate peroxidase, superoxide dismutase (SOD), catalase (CAT), peroxidase, glutathione-S-transferase (GST), ascorbic acid, and vitamin E are the most important components of the antioxidant defense mechanism 6. SOD is involved in the conversion of the superoxide radical (O 2 −) to H 2 O 2 , and the H 2 O 2 is eliminated by CAT and ascorbate peroxidase. GST is involved in eliminating H 2 O 2 from cells and lipid peroxidation agents 7,8 and also decreases the effects of toxins by binding xenobiotics to glutathione (GSH: γ-glutamyl-cysteinyl-glycine). GST is a member of the multifunctional phase II biotransformation multigene enzyme family that catalyzes the conjuga...

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Section: Discussionmentioning

confidence: 99%

Effects of Selenium and Vitamin E on Enzymatic, Biochemical, and Immunological Biomarkers in Galleria mellonella L

Coşkun

Kayış

Gulsu

et al. 2020

Sci Rep

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“…In addition, the reduction of THC may be a result of the inhibition of larval hematopoietic function and cell proliferation (Zhu et al, 2012). As suggested by many authors (Sharma et al, 2003;Sabri and Tariq, 2004;Tiwari et al, 2006;Pandey et al, 2007;Sendi and Salehi, 2010;Zhu et al, 2012;Zibaee et al, 2012), reduction of THC may be attributed to the cytotoxicity of botanicals and the death of pathologically degenerated cells.…”

Section: Decreasing Thcmentioning

confidence: 96%

“…On the other hand, decreasing THC had been reported in many insects by various plant extracts or plant-derived materials, such as the cotton bollworm Helicoverpa armigera by Artemisia annua, A. conyzoides, and Azadirachta indica oils (Padmaja and Rao, 2000); S. litura by Acorus calamus oils (Sharma et al, 2008); the lemon butterfly Papilio demoleus by leaf extracts of Eucalyptus globules, A. conyzoides and Allium sativum (Pandey et al, 2012); the red cotton stainer Dysdercus cingulatus after feeding of 5 th instar larvae on fresh food treated with 50% crude leaf extract of A. conyzoides (Pandey et al, 2007), as well as S. littoralis by certain concentrations of some compounds derived from urea and rice straw (Hassan et al, 2013). After feeding the rice moth Corcyra cephalonica larvae on rice treated with powdered leaves of Lantana camara, Clerodendrum inerme and Citrus limon, there was a remarkable reduction in the THC (Morya et al, 2010).…”

Section: Decreasing Thcmentioning

confidence: 99%

“…Over the last few decades, the worldwide research on insect circulating haemocytes has received much attention because these cells are responsible for different physiological functions, such as cell development and differentiation; metabolic processes; reproductive potential; endocrine regulation; distribution of nutritive materials and hormones to various sites throughout the insect body; coagulation to prevent loss of blood and wound healing; preservation of insect homeostasis; defense reactions against natural enemies invading the insect body cavity; as well as the detoxification of metabolites and other foreign materials (for some detail, see: Garcia and Rosales, 2002;Ceraul et al, 2003;Zhou et al, 2004;Ling et al, 2005;Ribeiro and Brehelin, 2006;Suhail et al, 2007;Strand, 2008;Pandey et al, 2010Pandey et al, , 2012Shaurub, 2012;Soares et al, 2013;Siddiqui and Al-Khalifa, 2014;Ghoneim et al, 2015aGhoneim et al, ,b,c,d, 2017Chavan et al, 2017;Er et al, 2017). In addition to these functions, insect hemocytes are very important for clearing apoptotic cells during development (Kurtz, 2002), as well as they are used as an excellent model system for the study of cell communication (Manogem et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…From the immunological point of view, hemocytes are very vital components of the insect immune system and are biochemically very sensitive having multiple functions, such as phagocytosis, encapsulation and nodulation, as defence mechanisms (Sorrentino et al, 2002;Ceraul et al, 2003;Ling et al, 2005;Figueiredo et al, 2006;Gandhe et al, 2007;Singh et al, 2008;Pandey et al, 2010Pandey et al, , 2012Vogelweith et al, 2016). In the last few decades, researc‫ا‬ers are devoting a great deal of work to haemocytes and their role in insect immunity (Jones, 1959;Gupta, 1979Gupta, , 1985Charalambidis et al, 1996;Tiwari et al, 2002;Ceraul et al, 2003;Pandey, 2004;Ling et al, 2005;Figueiredo et al, 2006;Gandhe et al, 2007;Merchant et al, 2008;Charroux and Royet, 2009;Pandey et al, 2010;Pandey and Tiwari, 2011;Shaurub, 2012;Ajamhassani et al, 2013;Blanco, 2016;Asiri, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Disturbing Effects of Botanicals on the Haemogram and Immune Parameters of Insects: Recent Progress of The Search for Effective Biopesticides

Ghoneim

Bakr

Hamadah

2021

Egyptian Academic Journal of Biological Sciences. A, Entomology

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List of Initials and Abbreviations………………. 5. Other parameters of haemogram as affected by botanicals…. 6. Immune responses and defense reactions of insects against Botanicals. 6.1. General outline ………………. 6.2. Insect immunocytes …………………… 6.3. Immuno-suppressive potential of botanicals on insects… 6.4. Major mechanisms of cellular innate immune responses in insects……. 6.5. Melanization as a humoral defense in insects … 6.6. Endocrine control of immune defenses ……. 7. Targeting the insect haemogram and immune reactions as a pest control strategy ….. 8. Summary points ………………… 9. Conclusions and future research needs ……………………… References ………………………………. List of Initials and Abbreviations: Abscisic acid (ABA), adiphohaemocytes (ADs), azadirachtin (Azt), blood volume (BV), coagulocytes (CGs), Differential haemocyte count (DHC), gibberellic acid (GA3), granulocytes (GRs), haemolymph volume (HV), 20-hydroxyecdysone (20E), indole-3acetic acid (IAA), juvenile hormone (JH), Mitotic index (MI), oenocytoids (OEs), phenoloxidase (PO), plant growth regulator (PGR), plasmatocytes (PLs), prohaemocytes (PRs), prophenoloxidase (proPO), scanning electron microscope (SEM), spherulocytes (SPs), Total haemocyte count (THC).

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Changes in immune responses of Helicoverpa armigera Hübner followed by feeding on Knotgrass, Polygonum persicaria agglutinin

Rahimi

Hajizadeh

Zibaee

et al. 2019

Arch Insect Biochem Physiol

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There is no study implying the effect of plant lectins on insect immune elements in both challenged and nonchallenged conditions with entomopathogenic agents. Lectins may bind to immune receptors on the surface of insect hemocytes, thus inducing or even disabling common immune functions including hemocyte counts, nodulation/encapsulation, phenoloxidase activity, and synthesis of antimicrobial peptides. In the present study, effect of Polygonum persicaria L. agglutinin (PPA) on immune responses of Helicoverpa armigera Hübner was investigated by feeding artificial diet treated to the larvae. Subsequently hemocyte count and expression of some immune-related genes were considered for analyses. The two groups of larvae including control and PPA-treated (1%) were divided into four subgroups of intact, Tween-80 injected, latex-bead injected and Beauveria bassiana-injected. Except for intact larvae, the highest numbers of total and differential hemocyte counts were recorded 12 hr postinjection, however, the PPA-fed larvae showed a significantly lower hemocyte counts compared to control.The number of nodules in PPA-fed larvae was significantly lower than control, but the injected larvae of both control and PPA showed the highest nodulation 24 hr postinjection.Although the highest activity of phenoloxidase was observed 12 and 24 hr postinjection but its activity significantly decreased in PPA-fed larvae compared to control. Gene expression of antimicrobial peptides including attacin, Arch. Insect Biochem. Physiol. 2019;101:e21543.wileyonlinelibrary.com/journal/arch

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Effect of Some Botanicals on Hemocytes and Molting of Papilio demoleus Larvae

Cited by 17 publications

References 13 publications

Effects of Selenium and Vitamin E on Enzymatic, Biochemical, and Immunological Biomarkers in Galleria mellonella L

Effects of Selenium and Vitamin E on Enzymatic, Biochemical, and Immunological Biomarkers in Galleria mellonella L

Disturbing Effects of Botanicals on the Haemogram and Immune Parameters of Insects: Recent Progress of The Search for Effective Biopesticides

Changes in immune responses of Helicoverpa armigera Hübner followed by feeding on Knotgrass, Polygonum persicaria agglutinin

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